Jurnal Logika, Vol 3, No 1 (2006)

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Efek Lama Pemberian Vitamin C Dosis Tinggi terhadap Aktivitas Hipoglikemia Glibenklamida pada Tikus

Endang Darmawan


An investigation has been done to study the effect of long-term oral doses vitamin c 100 mg/kgBW pretreatment on hypoglycemic effect of glibenclamide in sprague-dawley rat. The research was purely one-way completely random experimental design. The subject were 30 rat divided to 5 groups, 1st group administered using CMC Na 0,5 %, 2sd group using glibenclamide 0,45 mg/Kg BW, 3rd using vitamin C 100 mg/kgBW once time in day for 1 day and glibenclamide 100 mg/kgBW, 4th group using vitamin C 100 mg/kgBW once time in day for 3 days and glibenclamide 100 mg/kgBW, and 5th group administered vitamin C 100 mg/kgBW once time in day for 7 days and glibenclamide 100 mg/kgBW. Before administered by glibenclamide the subject was fasted during 18 hours. The hypoglycemic effect of glibenclamide after concomitant vitamin C 100 mg/kgBW was measured following an oral glucose tolerance test (GOTT). All of subject was get glucose 1,75 g/Kg BW 20 minutes after administered by glibenclamide. The concentration of blood glucose was established in 0 minute before GOTT and 15, 30, 60, 90, 120, 180, and 240 minutes of blood glucose concentration using enzymatic method used GOD-POD reagent. The data were analyzed statistically with one-way ANOVA, following Tukey test (p