A Highly Sensitive Electrochemical Glucose Sensor By Nickel-Epoxy Electrode With Non-Enzymatic Sensor

Riyanto Riyanto

Abstract

The preparation of new sensor for glucose was based on the fact that glucose can be determined by non-enzymatic glucose oxidase. The Ni metals (99.98% purity, 0.5 mm thick, Aldrich Chemical Company) was used to prepare Ni-Epoxy electrode. The Ni-epoxy electrodes were prepared in square cut of 1 cm and 1 mm by length and wide respectively. The Ni metal electrodes were connected to silver wire with silver conducting paint prior covered with epoxy gum. The prepared of nickel-epoxy modified electrode showed outstanding electro catalytic activity toward the oxidation of glucose in alkaline solution. The result from this research are correlation of determination using Nickel-Epoxy
electrode for electroanalysis of glucose in NaOH was R2 = 0.9984. LOQ, LOD and recovery of the Nickel-Epoxy electrode towards glucose were found to be 4.4 μM, 1.48 μM and 98.19%, respectively. The Nickel-Epoxy wire based electrochemical glucose sensor demonstrates good sensitivity, wide linear range, outstanding detection limit, attractive selectivity, good reproducibility, high stability as well as prominent feasibility use of non-enzymatic sensor for monitoring glucose in human urine owing to its advantages of low cost, simple preparation and excellent properties for glucose detection.

Keywords

glucose; sensor; nickel-epoxy; non-enzymatic

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References

Chung, T.D. Park, S. and Boo, H., 2006, Electrochemical non-enzymatic glucose sensors, Anal. Chim. Acta, 556, 46–57.

Ding, Y. Wang, Y. Su, L. Zhang, H. and Lei, Y., 2010, Preparation and characterization of NiO–Ag Nanofibers, NiO nanofibers, and porous Ag: towards the developmentof a highly sensitive and selective non-enzymatic glucose sensor, J. Mater. Chem. 20, 9918–9926.

Newman, J.D. and Turner, A.P.F., 2005, Home blood glucose biosensors: a commercial per-spective, Biosens. Bioelectron. 20, 2435–2453.

Nie, H.G. Yao, Z. Zhou, X.M. Yang, Z. and Huang, S.M., 2011, Nonenzymatic electrochemical detection of glucose using well-distributed nickel nanoparticles on straight multi-walled carbon nanotubes, Biosens. Bioelectron. 30, 28–34.

Peng, X. Yu, S.J. Cao, G.Z. Zhou, M. Qiao, L. Yao, J.Y. and He, H.C., 2012, Ni nanoparticles decorated titania nanotube arrays as efficient nonenzymatic glucose sensor, Electrochim. Acta, 76, 512–517.

Pisoshi, A.M., Danet, A.F., and Negulscu, G.P., 2007, Influense of the buffer capacity on glucose potentiometric determination in synthetic solutions and in real sampels with different acidities, Pro. Rom, Acad., Series B, 75-81.

Shervedani, R.K. Motahareh. K. Akbar. A and Prickly, 2014, Nickel nanowires grown on Cu substrate as a supersensitive enzyme-free electrochemical glucose sensor, Sensor and actuator B: Chemical, 204, 783-790.

Shervedani, R.K. Mehrjardi, A.H., 2007, Electrochemical characterization of directly immobilized glucose oxidase on gold mercaptosuccinic anhydride self-assembled monolayer, Sens. Actuators B 126, 415–423.

Sun, A. Zheng, J. and Sheng, Q., 2012, A highly sensitive non-enzymatic glucose sensor based on nickel and multi-walled carbon nanotubes nanohybrid filmsfabricated by one-step co-electrodeposition in ionic liquids, Electrochim. Acta, 65, 64–69.

Tian, H. Jia, M. Zhang, M. and Hu, J., 2013, Nonenzymatic glucose sensor based on nickel ion implanted-modified indium tin oxide electrode, Electrochim. Acta. 96, 285–290.

Turner, A.P.F. and Wilson, R. 1992, Glucose oxidase: an ideal enzyme, Biosens. Bioelectron. 7, 165–185.

Veeramani,V., Madhua,R., Chena,S.M., ,Veerakumar,P., Hungb, C.T, and Liub, S.B., 2015, Heteroatom-enriched porous carbon/nickel oxide nanocomposites as enzyme-free highly sensitive sensors for detection of glucose, Sensor and Actuators: B, 1384-1390

Wang, J., 2008, Electrochemical glucose biosensors, Chem. Rev. 108, 814–825.

Wang, L. Lu, X. Ye, Y. Sun, L. and Song Y., 2013, Nickel-cobalt nanostructures coated reduced graphene oxide nanocomposite electrode for nonenzymatic glucose biosensing, Electrochim. Acta 114, 484–493.

Yang. H., Chung. T.D. Kim, Y.T. Choi, C.A. Jun, C.H. and Kim, H.C. 2002, Glucose sensor using a microfabricated electrode and electropolymerized bilayer films, Biosens, Bioelectron,17, 251-259.



Eksakta: Jurnal Ilmu-Ilmu MIPA
ISSN 1411-1047 (print), ISSN 2503-2364 (online)
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Faculty of Mathematics and Natural Science
Universitas Islam Indonesia, Yogyakarta

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