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Abstract
ABSTRACT
Zeorin or 6,22-Dihydroxyhopane is a compound isolated from Aegle marmelos Correa
collected in Yogyakarta Indonesia. The molecular structure was confirmed in Universiti Putra
Malaysia. This compound was obtained from petroleum ether extract of the leaves of Aegle
marmelos Correa. In present study, we investigated the effects of zeorin on the -hexoaminidase
enzyme release from mast cell culture. The experiment was performed by using rat basophilic
leukemia (RBL-2H3) cell line, a tumor analog of mast cells. DNP24-BSA and thapsigargin were used
as immunologic and non-immunologic inducers for -hexoaminidase enzyme release from mast
cells, respectively. The release of -hexoaminidase enzyme was determined by using colorimetric
methods with an enzyme substrate, p-nitrofenil-2-Acetamido-2-deoksi--D-gluko-piranosida, and a
microplate reader at 405 nm. In this study, treatment of 20 ng/mL DNP24-BSA and 0.5 M
thapsigargin could stimulate the release of -hexoaminidase enzyme from RBL-2H3 cells by
25.421.62 % and 33.163.72 %, respectively. Zeorin showed potent inhibitory effects on the -
hexoaminidase enzyme release, when the release induced by DNP24-BSA. In contrast, zeorin show
weak inhibitory effects, when the -hexoaminidase enzyme release from RBL-2H3 cells induced by
a Ca
2+
stimulant, thapsigargin. The IC50 values of zeorin’s effects on DNP24-BSA and thapsigargin
experiments were 33,71 M and >100 M, respectively. Based on the results, the inhibitory effect of
zeorin on the -hexoaminidase enzyme release from RBL-2H3 cells involving mechanisms related
to the interaction of IgE on the mast cell surface or intracellular signal transductions involved in
mast cell degranulation.
Key words : Aegle marmelos Correa, zeorin, sel mast, -hexoaminidase enzyme