Hepatoprotective Effect of Corn Silk Infusion in Male Wistar Rats

Arba Pramundita Ramadani, Hady Anshory Tamhid, Tika Luthfi Sadrina, Jasno Jasno, Mabrurotul Mustafidah


The use of medicinal plants in treating various disease has been reported since long time ago, including for hepatic disease. Corn silk contains phytochemicals of medical benefit such as flavonoids compounds which act as antioxidant agents and has been widely reported possess hepatoprotective effect. Using a model of carbon tetrachloride (CCl4)-induced hepatotoxicity in 36 male Wistar rats, this study investigated the effect of corn silk infusion and assessed using enzymes produced by the liver in plasma [alkaline phosphatase (ALP) and liver glutathione (GSH)]. The corn silk infusion (in 50, 100, and 200 mg/kg BW doses) were administered 24 hours after CCl4-induction liver damage with 3ml/kg BW CCl4 in olive oil (1:1, v/v), intraperitoneally for seven days. Along with corn silk groups, distilled water (0.2 mL/kg BW) and Curcumin (100 mg/kg BW) were given for induction and drug control, respectively. In the end of the study (8th day), the level of both ALP dan GSH were measured. The differences among groups for GSH and ALP level were statistically calculated using ANOVA method.  The result showed that the corn silk infusion is active at 200 mg/kg BW based on both ALP (18.74% decreased) and GSH (5-7% increased) level. Moreover, the flavonoid compound was detected on the infusion that may contribute on its hepatoprotective activity. In conclusion, corn silk infusion owned hepatoprotective effect in male Wistar rats.


corn silk, hepatoprotective, ALP, GSH

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G. M. Cragg, D.J. Newman and K. M. Snader, Natural products in drug discovery and development. J Nat Prod, 60 (1) (1997) 52-60

B. G. Schuster, Demonstrating the validity of natural products as anti-infective drugs. J Altern Complement Med, 7 (1) (2001) 73-82.

H. P, Zhao, et al., Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk. Biomed Pharmacother, 90 (2017) 686-693.

Lee, J., et al., Immunostimulating activity of maysin isolated from corn silk in murine RAW 264.7 macrophages. BMB Rep, 47(7) (2014) 382-7.

S. Morshed and S.M.S. Islam, Antimicrobial activity and phytochemical properties of corn (Zea mays L.) silk. SKUAST J of Res, 17(1) (2015) 8-14.

S. Zilic, et al., Antioxidant activity, phenolic profile, chlorophyll and mineral matter content of corn silk (Zea mays L.): Comparison with medicinal herbs. J Cereal Sci, 69 (2016) 363-370.

E. Sarepoua, R. Tangwongchai, B. Suriharn and K. Lertrat, Influence of variety and harvest maturity on phytochemical content in corn silk. Food Chem, 15 (169) (2015) 424-429.

M. Karami, S. Saeidnia, and A. Nosrati, Study of the Hepatoprotective Activity of Methanolic Extract of Feijoa sellowiana Fruits Against MDMA using the Isolated Rat Liver Perfusion System. Iran J Pharm Res, 12(1) (2013) 85-91.

E. G. Giannini, R. Testa, and V. Savarino, Liver enzyme alteration: a guide for clinicians. CMAJ, 172(3) (2005) 367-79.

P. Di Mascio, M.E. Murphy, and H. Sies, Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols. Am J Clin Nutr, 53(1) (1991) 194-200.

M. Hasan, N. Tamanna, and A. Haque, Biochemical and histopathological profiling of Wistar rat treated with Brassica napus as a supplementary feed. Food Science and Human Wellness, 7(1) (2018) 77-82.

A. M. Mohamadin, et al., Attenuation of oxidative stress in plasma and tissues of rats with experimentally induced hyperthyroidism by caffeic acid phenylethyl ester. Basic Clin Pharmacol Toxicol, 100 (2) (2007) 84-90.

C.C Chang, et al., The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity. BMC Complement Altern Med,16

(432) (2016) 1-11.

Y. Zhang, L. Wu, Z. Ma, J. Cheng and J. Liu, Anti-Diabetic, Anti-Oxidant and Anti-Hyperlipidemic Activities of Flavonoids from Corn Silk on STZ-Induced Diabetic Mice. Molecules, 21 (7) (2016) 1-11.

M. A. Ebrahimzadeh, et al., Correlation between Sun Protection Factor and Antioxidant Activity, Phenol and Flavonoid Contents of some Medicinal Plants. Iran J Pharm Res, 13(3) (2014) 1041-1047.

J. Tian, et al., Comparative studies on the constituents, antioxidant and anticancer activities of extracts from different varieties of corn silk. Food Funct, 4 (10) (2013) 1526-1534.

K. Hasanudin, P. Hashim, and S. Mustafa, Corn silk (Stigma maydis) in healthcare: a phytochemical and pharmacological review. Molecules, 17 (8) (2012) 9697-9715.

N. A. Rahman, and W.I.W. Rosli, Nutritional compositions and antioxidative capacity of the silk obtained from immature and mature corn. Journal of King Saud University, 26(2) (2014) 119-127.

B. Halliwell, Antioxidant characterization. Methodology and mechanism. Biochem Pharmacol, 49 (10) (1995) 1341-1348.

J. L. Millan, Alkaline Phosphatases : Structure, substrate specificity and functional relatedness to other members of a large superfamily of enzymes. Purinergic Signal, 2(2) (2006) 335-341.

U. Sharma, D. Pal, and R. Prasad, Alkaline phosphatase: an overview. Indian J Clin Biochem, 29 (3) (2014) 269-278.

N. Kaplowitz, The importance and regulation of hepatic glutathione. Yale J Biol Med, 54 (6) (1981) 497-502.

L. W. Weber, M. Boll, and A. Stampfl, Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Crit Rev Toxicol, 33 (2) (2003) 105-136.

Bai, H., et al., Protective effect of maize silks (Maydis stigma) ethanol extract on radiation-induced oxidative stress in mice. Plant Foods Hum Nutr, 65(3) (2010) 271-276.

O. Katikova, V. Kostin Ia, and V.S. Tishkin, [Hepatoprotective effect of plant preparations]. Eksp Klin Farmakol, 65(1) (2002) 41-43.

K. Hasanudin, K., P. Hashim, and S. Mustafa, Corn silk (Stigma maydis) in healthcare: a phytochemical and pharmacological review. Molecules (Basel, Switzerland), 17 (8) (2012) 9697-9715.

L. A. Pham-Huy, L.A., H. He, and C. Pham-Huy, Free radicals, antioxidants in disease and health. Int J Biomed Sci, 4 (2) (2008) 89-96.

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