Main Article Content
Abstract
Curcuma mangga Val. is one of Indonesian herbs from Zingiberaceae family that is under explored and could contain potentially active substances to serve as an antimalarial. This research intends not only to examine the antimalarial activity by means of heme polymerization inhibitor mechanism by using the ethanolic extract and fraction of Curcuma mangga Val. but also to identify its compound classification. The extract of temu manga was obtained by Soxhlet extraction method using ethanol solvent followed by fractionation using Vacuum Liquid Chromatography with solvent sequence n-hexane, n-hexane: ethyl acetate (2:1), ethyl acetate and ethanol. The extract and fraction were analyzed by using LC-MS and GC-MS. Activities of hem polymerization inhibition showed by IC50 values which were obtained from analysis of relationship between concentration sample and the percentage of inhibition using the PROBIT on statistical software. The result of HPIA assay shows that the IC50 value of ethanolic extract and ethanolic fraction of Curcuma mangga Val. rhizome are 2.273 and 1.479 mg/mL, respectively. It clearly shows that the heme polymerization inhibition activity of ethanolic fraction relatively better than that of ethanolic extract. Phytochemical screening determines the ethanolic extract contains saponin, terpenoid, and phenol while the ethanolic fraction contains terpenoid. Thus, terpenoid compound is presumed to be the inhibitor of heme polymerization. The results of analysis with LC-MS and GC-MS showed that the active compounds suspected to inhibit heme polymerization in ethanolic extract and fraction were (E) -labda-8 (17), 12-dien-15,16-dial and di-n-octyl phthalate, respectively.
Keywords: antimalarial, Curcuma mangga Val., heme polymerization
Keywords
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References
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References
WHO, World health statistics 2017: monitoring health for the SDGs, Sustainable Development Goals,
Geneva, 2017.
M. Figtree, R. Lee, L. Bain, T. Kennedy, S. Mackertich, M. Urban, Q. Cheng, B.J. Hudson,
Plasmodium knowlesi in human, Indonesian Borneo, Emerging Infectious Diseases, 16 (2010) 672–674
G. Geleta, T. Ketema, Severe Malaria Associated with Plasmodium falciparum and P. vivax among
Children in Pawe Hospital, Northwest Ethiopia, Malaria Research and Treatment. 2016 (2016) 1–7.
WHO, World Health Statitics-Monitoring health for The SDGs, Geneva, 2016
Y Yuandani, A. Dalimunthe, P, P. A. Z. Hsb, A. W. Septama, Uji Aktivitas Antikanker (Preventif dan Kuratif) Ekstrak Etanol temu Mangga (Curcuma Mangga Val) pada Mencit yang Diinduksi Siklofosfamid, Majalah Kesehatan Pharmamedika. 3 (2011) 255-259
S. N. A. Malek, G.S. Lee, S. L. Hong, H. Yaacob, N.A. Wahab, J. F. F. Weber, S. A. A. Shah, Phytochemical and cytoxic investigations of curcuma mangga rhizomes, Molecules. 16 (2011) 4539-4548
D. Fitriastuti, A. W. N. Iman, D. A. Lutfiani, D. Yuliyanti, In Vitro antiplasmodial activity of extract and fraction of temu mangga (Curcuma mangga) against plasmodium falciparum 3D7, AIP Conference Proceeding. 2026 91) (2018) 020105-1-020105-7
N. basilico, E. Pagani, D. Monti, P. Olliaro, D. Taramelli, A microtitre-based methos for measing the haem polymerization inhibitory activity (HPIA) of antimalarial drugs, Journal of Antimicrobial Chemotheraphy
K. Kusmiyati, N. Aznam, S. Handayani, Isolasi dan Identifikasi Zat Aktif Ekstrak Metanol Rimpang Kunyit Putih (Curcuma mangga Val) fraksi Etil Asetat, Pharmaciana. 1 (2011) 1-10
F. Abas, N. H. Lajis, K. Shaari, D. A. Israf, J. Stanslas, U. K. Yusuf, S.M. Raof, A labdane diterpene glucoside from the rhizomes of curcuma mangga, Journal of Natural Products. 68 (2005) 1090-1093
S. R. Hawley, P. G. Bray, M. Mungthin, J.D. Atkinson, P. M. O'neill, S.A. Ward, Relationship between antimalarial drug activity, accumulation and inhibitation of heme polymerization in plasmodium falciparum in vitro, Antimicrobial Agents and Chemitherapy. 42 91998) 682-686
S. Parapini, N. Basilico, E. Pasini, T.J. Egan, P. Olliaro, D. taramelli, D. Monti, Stardardization of the physicochemical parameters to assess in vitri the B-hematin in hibitory activity of antimalarial drugs, Experimental Parasitology, 96 (2000) 249-256
Y. Kurosawa, A. Dorn, M. Kitsuji-Shirane, H. Shimada,, T. Satoh, H. matile, W. Hofheinz, R. Masciadri, M. kansy, R.G. Ridley, Hematin polymerization assay as high-throughput screen for identification of new antimalarial pharmacophores, Antimicrobial Agents and Chemotherapy. 44 (2000) 2638-2644
S. Kumar, M. Guha,V. Choubey, P. Maity, U. Bandyopadhyay, Antimalarial drugs inhibiting hemozoin (B-hematin) formation: A mechanistic update, Life Sciences. *0 (2007) 813-828
R. Baelmans, E. Deharo, V. Muoz, M. Sauvain, H. Ginsburg, Experimental parasitology. 96 (2000) 243-248
R. Muti'ah, penyakit Malaria dan Mekanisme Kerja Obat-obat Antimalaria, Alchemy. 2 (2013) 80-91
A. A. Imam, M.D. Ezema, I.U. Muhammad, M. k. Atiku, A. J. Alhassan, A. Idi, H. Abdullahi, A. Mohammed, In vivo antimalarial activity of solvents extract of alstonia boonei stem bark and partial characterization of most active extracts, Annual Research & Review in Biologu. 17 (2017) 1-11
P. M. O'Neill, V. E. Barton, S. A. Ward, the molecular mechanism of action of artemisinin The debate continues, Molecules. 15 (2010) 1705-1721
P. Sonnet, C. Mullie, In vitro antimalarial activity of ICL670: A further proof of the correlation between inhibition of B-hemation formation and peroxidative degradation of hemin, Experimental Parasitology. 128 (2011) 26-31