Article Sidebar

Submitted
June 26, 2020
Accepted
August 26, 2020
Published
September 7, 2020
Download
PDF
Statistic
Read Counter : 504 Download : 441

Main Article Content

Abstract

This study aimed to detection of protein profile, expression of metalothionein (MT) protein, and analyze difference of MT density in liver and gill in one of Carp fish that is Barbonymus balleroides in the upstream and downstream of Brantas river. The method used observasional analytic, Two individual of Barbonymus balleroides samples taken three times (with difference of month) from two station there are upstream and downstream of Brantas river. Analysis protein profile used electrophoresis SDS-PAGE (15%), expression and density of MT used western blot method and imageJ software. Difference between MT density in liver and gill of Barbonymus balleroides in the upstream and downstream of Brantas river analyzed with Two-way ANOVA. The results showed, protein profile in liver and gill Barbonymus balleroides in the upstream and downstream Brantas river have molecular weight about 8-93 kDa, expression of MT showed band of MT with molecular weight 24 kDa, results of MT density in liver and gill on upstream and downstream, continuously 231.29 MT/µm2 and 229.87MT/µm2, 232.41 MT/µm2 and  231.56 MT/µm2 but there is not significant.

Keywords

Brantas river metallothionein liver gills Carp fish Barbonymus balleroides.

Article Details

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
Setiyowati, P. A. I., Solekha, R., Sahara, S. B., & Hapsari, F. D. (2020). Analysis Metallothionein of Carp fish in The Brantas River, Indonesia. EKSAKTA: Journal of Sciences and Data Analysis, 20(2), 139–146. https://doi.org/10.20885/EKSAKTA.vol1.iss2.art8
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

References

  1. M. Radhakrishnan and M. Jithesh, Seasonal variation in accumulation of metals in selected tissuesof the Ribbon fish, Trichiurus lepturus collected from Chaliyar River, Kerala, India. Journal ofEntomology and Zoology Studies, 5(51)(2017), 51.
  2. R. W. Sayekti et al., “Studi Evaluasi Kualitas Dan Status Trofik Air Waduk Selorejo Akibat ErupsiGunung Kelud Untuk Budidaya Perikanan,” Tek. Pengair., 6 (1)(2015), 1.
  3. J. F. P. Oaten, M. D. Hudson, A. C. Jensen, and I. D. Williams, “Seasonal effects to metallothioneinresponses to metal exposure in a naturalised population of Ruditapes philippinarum in a semi-enclosed estuarine environment,” Sci. Total Environ., 575(2017).
  4. A. A. Bawuro, R. B. Voegborlo, and A. A. Adimado, “Bioaccumulation of Heavy Metals in Some Tissues of Fish in Lake Geriyo, Adamawa State, Nigeria,” J. Environ. Public Health, (2018).
  5. E. M’Kandawire et al., “Metallothionein from wild populations of the African catfish Clariasgariepinus: From sequence, protein expression and metal binding properties to transcription albiomarker of metal pollution,” Int. J. Mol. Sci., 18 (7)(2017), 7.
  6. C. S. Carvalho, H. S. M. Utsunomiya, T. Pasquoto, R. Lima, M. J. Costa, and M. N. Fernandes,“Blood cell responses and metallothionein in the liver, kidney and muscles of bullfrog tadpoles, Lithobates catesbeianus, following exposure to different metals,” Environ. Pollut., 221(2017).
  7. A. Hayati et al., “Metallothionein analysis and cell damage levels on the liver and gill Of Barbonymusgonionotus In Brantas River, Indonesia,” J. Biol. Res.,23 (1)(2017), 1.
  8. I. Rosadi, M. Syaifudin, and D. Elfidasari, “Protein Profile and Hematological Parameters of Micepost Injected with Irradiated Plasmodium berghei,” Biosaintifika J. Biol. Biol. Educ., 8(2)(2016), 2.
  9. M. Niederwanger et al., “Challenging the Metallothionein (MT) gene of Biomphalaria glabrata:Unexpected response patterns due to cadmium exposure and temperature stress,” Int. J. Mol. Sci., 18(8)(2017), 8.
  10. A. G. M. Osman, S. Wuertz, and K. Mohammed-Geba, “Lead-induced heat shock protein(HSP70)and metallothionein (MT)gene expression in the embryos of African catfish Clariasgariepinus (Burchell, 1822),” Sci. African,3(2019).
  11. M. V. Clausen, F. Hilbers, and H. Poulsen, “The structure and function of the Na,K-ATPase isoformsin health and disease,” Front. Physiol., 8(6)(2017), 6.
  12. A. A. Bawuro, R. B. Voegborlo, and A. A. Adimado, “Bioaccumulation of Heavy Metals in SomeTissues of Fish in Lake Geriyo, Adamawa State, Nigeria,” J. Environ. Public Health, (2018).
  13. G. Azeh Engwa, P. Udoka Ferdinand, F. Nweke Nwalo, and M. N. Unachukwu, “Mechanism andHealth Effects of Heavy Metal Toxicity in Humans,” Poisoning Mod. World -New Tricks an Old Dog?,(6)(2019).[14] N. I. Rahayu et al., “Pengaruh paparan Timbal (Pb) terhadap laju pertumbuhan ikan nila(Oreochromis nilloticus),” Jimvet, 1(4)(2017), 4.
  14. C. Thanomsit, “Monitoring of heavy metal contamination in aquatic organism by applyingMetallothionein biomarker and its situation in Thailand,” Naresuan Univ. J. Sci. Technol.,24(1)(2016),1
  15. K. Duncan, “Metallothioneins and Related Chelators. Metal Ions in Life Sciences Vol. 5. Edited byAstrid Sigel, Helmut Sigel and Roland K. O. Sigel.,” Angew. Chemie Int. Ed., 48(43)(2009), 43.
  16. M. A. Mehmood et al., “Heavy metal contamination in two commercial fish species of a trans-Himalayan freshwater ecosystem,” Environ. Monit. Assess., 191(2)(2019), 2.
  17. M. S. Bhuyan, M. A. Bakar, M. Rashed-Un-Nabi, V. Senapathi, S. Y. Chung, and M. S. Islam,“Monitoring and assessment of heavy metal contamination in surface water and sediment of the OldBrahmaputra River, Bangladesh,” Appl. Water Sci., 9(5)(2019), 5.
  18. E. Antai, A. Joseph, A. Bassey, and F. Okoro, “The influence of size and seasons on the bio-accumulation of heavy metals in tissues of Clarias gariepinus from QUA IBOE River, Southeastern Nigeria,” Int. J. Zool. Stud., 2(1)(2017),1.