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Abstract
Thiamine is a crucial cofactor in energy metabolism. Deficiency of this vitamin can cause disorders in the nervous and cardiovascular systems. Conventional methods for examining thiamine levels in the body are expensive. One solution is using a technique similar to ELISA, namely ELPLA, which uses thiamine-binding protein as an antibody substitute. This study aims to investigate the presence of thiamine-binding protein in black glutinous rice bran (Oryza Sativa var. Glutinosa). The methods used include salting out, dialysis, and equilibrium dialysis. The results show the presence of thiamine-binding protein in black glutinous rice bran, evidenced by equilibrium dialysis.
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Copyright (c) 2024 Dwirini Gunarti, Josua Kristinao Hilmanto
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References
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- Sullivan, K. (2002). Vitamins and minerals: A practical approach to a health diet and safe supplementation.
- Food and Nutrition Board US Institute of Medicine (The National Academies Press. Nutrition - Dietary Reference Intakes: DRIs): Recommended Dietary Allowances and Adequate Intakes, Vitamins. Available at
- Román GC, Román GC. Ariza Prison: Cienfuegos, Cuba, August 5, 1992. Cuba Blind. 2016 Jan 1;11–8.
- Gunarti DR, Rahmi H, Sadikin M. Isolation and Purification of Thiamine Binding Protein from Mung Bean. HAYATI J Biosci. 2013 Mar 1;20(1):1–6.
- Anyakora C, Afolami I, Ehianeta T, Onwumere F. HPLC analysis of nicotinamide, pyridoxine, riboflavin and thiamin in some selected food products in Nigeria. Afr J Pharm Pharmacol; 2008:2(2):29–36.
- Chen Y, Tian F. Enzymatic Catalytic Spectrophotometric Determination of Thiamine in Food. Food Anal Methods. 2010 Mar 28;3(1):7–11.
- Wingfield P. Protein precipitation using ammonium sulfate. Curr Protoc protein Sci. 2001 May;Appendix 3:Appendix 3F.
- Rosenberg IM. Protein analysis and purification. 2nd ed. Boston: Birkhäuser; 1996. Chapter 5, Getting started with protein purification; p. 118–52.
- Simonian MH. Spectrophotometric Determination of Protein Concentration. Curr Protoc Cell Biol. 2002 Jul;15(1):A.3B.1-A.3B.7.
- Barwick V. Preparation of calibration curves: a guide to best practice. LGC/VAM/2003/032. Available from: https://www.lgcgroup.com/media/1735/prepration-of-calibration-curves_a-guide-to-best-practice.pdf.
- Analytical chemistry: calibration curves. Cambridge, MA: JoVE Science Education Database; 2019 [cited 2091 Oct 28]. Available from: https://www.jove.com/science-education/10188/calibration-curves.
- N K. Methods in enzymology. Acad Press. 1968;I:76.
- Shimizu M, Yoshida T, Toda T, Iwashima A, Mitsunaga T. Isolation of a Thiamine-binding Protein from Rice Germ and Distribution of Similar Proteins. Biosci Biotechnol Biochem. 1996 Jan 12;60(3):453–7.
- Adachi T, Watanabe K, Mitsunaga T. Characterization of thiamin-binding protein from maize seeds. Plant Physiol Biochem. 2001 Feb 1;39(2):99–105.
- Watanabe K, Konishi A, Mitsunaga T. Molecular characteristics of thiamin-binding protein from sunflower seeds. Plant Physiol Biochem. 2002 May 1;40(5):417–21
- Rosenberg IM. 2005. Protein Analysis and Purification. Boston: BirkhÓuser.
- Mohammad F. Hossain, Mamoon Rashid, Rajjit Sidhu, Randy Mullins, and Susan L. A Simplified, Specific HPLC Method of Assaying Thiamine and Riboflavin in Mushrooms., Int J Food Sci. 2019; 2019: 8716986. Published online 2019 Feb 3. doi: 10.1155/2019/8716986, PMCID: PMC6378034, PMID: 30854396
- Mitsunaga T, Shimizu M, Iwashima A. Occurrence of thiamine-binding proteins in plant seeds. J Plant Physiol. 1986 Jun 1;124(1–2):177–80.
- Mitsunaga T, Matsuda M, Shimizu M, Iwashima A. Isolation and properties of a thiamine-binding protein from buckwheat seed. Cereal Chem. 1986;63:332–5.
References
Page GLJ, Laight D, Cummings MH. Thiamine deficiency in diabetes mellitus and the impact of thiamine replacement on glucose metabolism and vascular disease. Int J Clin Pract. 2011 Jun;65(6):684–90.
Sullivan, K. (2002). Vitamins and minerals: A practical approach to a health diet and safe supplementation.
Food and Nutrition Board US Institute of Medicine (The National Academies Press. Nutrition - Dietary Reference Intakes: DRIs): Recommended Dietary Allowances and Adequate Intakes, Vitamins. Available at
Román GC, Román GC. Ariza Prison: Cienfuegos, Cuba, August 5, 1992. Cuba Blind. 2016 Jan 1;11–8.
Gunarti DR, Rahmi H, Sadikin M. Isolation and Purification of Thiamine Binding Protein from Mung Bean. HAYATI J Biosci. 2013 Mar 1;20(1):1–6.
Anyakora C, Afolami I, Ehianeta T, Onwumere F. HPLC analysis of nicotinamide, pyridoxine, riboflavin and thiamin in some selected food products in Nigeria. Afr J Pharm Pharmacol; 2008:2(2):29–36.
Chen Y, Tian F. Enzymatic Catalytic Spectrophotometric Determination of Thiamine in Food. Food Anal Methods. 2010 Mar 28;3(1):7–11.
Wingfield P. Protein precipitation using ammonium sulfate. Curr Protoc protein Sci. 2001 May;Appendix 3:Appendix 3F.
Rosenberg IM. Protein analysis and purification. 2nd ed. Boston: Birkhäuser; 1996. Chapter 5, Getting started with protein purification; p. 118–52.
Simonian MH. Spectrophotometric Determination of Protein Concentration. Curr Protoc Cell Biol. 2002 Jul;15(1):A.3B.1-A.3B.7.
Barwick V. Preparation of calibration curves: a guide to best practice. LGC/VAM/2003/032. Available from: https://www.lgcgroup.com/media/1735/prepration-of-calibration-curves_a-guide-to-best-practice.pdf.
Analytical chemistry: calibration curves. Cambridge, MA: JoVE Science Education Database; 2019 [cited 2091 Oct 28]. Available from: https://www.jove.com/science-education/10188/calibration-curves.
N K. Methods in enzymology. Acad Press. 1968;I:76.
Shimizu M, Yoshida T, Toda T, Iwashima A, Mitsunaga T. Isolation of a Thiamine-binding Protein from Rice Germ and Distribution of Similar Proteins. Biosci Biotechnol Biochem. 1996 Jan 12;60(3):453–7.
Adachi T, Watanabe K, Mitsunaga T. Characterization of thiamin-binding protein from maize seeds. Plant Physiol Biochem. 2001 Feb 1;39(2):99–105.
Watanabe K, Konishi A, Mitsunaga T. Molecular characteristics of thiamin-binding protein from sunflower seeds. Plant Physiol Biochem. 2002 May 1;40(5):417–21
Rosenberg IM. 2005. Protein Analysis and Purification. Boston: BirkhÓuser.
Mohammad F. Hossain, Mamoon Rashid, Rajjit Sidhu, Randy Mullins, and Susan L. A Simplified, Specific HPLC Method of Assaying Thiamine and Riboflavin in Mushrooms., Int J Food Sci. 2019; 2019: 8716986. Published online 2019 Feb 3. doi: 10.1155/2019/8716986, PMCID: PMC6378034, PMID: 30854396
Mitsunaga T, Shimizu M, Iwashima A. Occurrence of thiamine-binding proteins in plant seeds. J Plant Physiol. 1986 Jun 1;124(1–2):177–80.
Mitsunaga T, Matsuda M, Shimizu M, Iwashima A. Isolation and properties of a thiamine-binding protein from buckwheat seed. Cereal Chem. 1986;63:332–5.