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Submitted
June 29, 2024
Accepted
September 17, 2024
Published
October 30, 2024
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Abstract

Thiamine is a crucial cofactor in energy metabolism. Deficiency of this vitamin can cause disorders in the nervous and cardiovascular systems. Conventional methods for examining thiamine levels in the body are expensive. One solution is using a technique similar to ELISA, namely ELPLA, which uses thiamine-binding protein as an antibody substitute. This study aims to investigate the presence of thiamine-binding protein in black glutinous rice bran (Oryza Sativa var. Glutinosa). The methods used include salting out, dialysis, and equilibrium dialysis. The results show the presence of thiamine-binding protein in black glutinous rice bran, evidenced by equilibrium dialysis.

Keywords

Thiamine-Binding Protein Isolation Equilibrium Dialysis Black Glutinous Rice Bran

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Copyright (c) 2024 Dwirini Gunarti, Josua Kristinao Hilmanto

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How to Cite
Gunarti, D., & Josua Kristinao Hilmanto. (2024). The Isolation and Identification of Thiamine-Binding Protein from Black Glutinous Rice Bran (Oryza Sativa var. Glutinosa). EKSAKTA: Journal of Sciences and Data Analysis, 5(2), 162–170. https://doi.org/10.20885/EKSAKTA.vol5.iss2.art6
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