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Abstract
Stevia, a plant commonly used as a natural sweetener, has the potential to be used as a sugar substitute because the stevioside content provides high sweetness and has a low caloric value. On the other hand, stevia contains tannins that can give it a bitter taste. To reduce the tannin content in stevia extract, tannin is coagulated with calcium metal ions from lime and then passed into a ion exchange resin column. The purpose of this study was to determine the effect of lime concentration on the reduction of tannin levels and the efficiency of resin columns in reducing tannin levels. However, it is expected that the extract still has a high level of sweetness. Extraction was carried out on dried stevia leaves using water solvent in a ratio of 1:30 (w/v). After coagulation with lime, the extract was filtered and then passed to the resin column to bind the remaining calcium ions. The concentration of lime was varied: 0,5; 1; 1,5; 2; 2,5; 3; 4; 5; 6 %. The tannin content in the extract, before and after the process was tested by spectrophotometric method using Folin Ciocalteu reagent. The sweetness level of the extract was tested by measuring the % brix value using a brix refractometer. The results showed that the high efficiency of significant tannin content reduction was 99.98% with a minimum sweetness level reduction of 47.1% at 2% (w/v) lime addition.
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Copyright (c) 2025 Riniati Polban, Sudrajat Harris Abdulloh, Endang Widiastuti, Ari Marlina
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References
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References
R. R. Sheeja And B. Lawrence, “Phytochemical Screening Of The Leaves Of Stevia Rebaudiana, Bertoni,” Int.J.Curr.Microbiol.App.Sci, Vol. 4, No. 3, Pp. 344–347, 2015.
C. Setiawan And N. J. Asrilya, “Preparasi Dan Karakterisasi Senyawa Tanin Dari Daun Stevia (Stevia Rebaudiana) Menggunakan Instrumen Hplc Sebagai Gula Pereduksi Dalam Pembuatan Sukrosa,” Walisongo J. Chem., Vol. 3, No. 2, P. 86, 2020, Doi: 10.21580/Wjc.V3i2.6591.
I. D. Ghazi, “Penghilangan Warna Coklat Gula Stevia Menggunakan Karbon Aktif,” Vol. 2, No. 4, Pp. 198–204, 2013.
Sinta Nur Laili Fatmawati, “Pengaruh Penggunaan Adsorbens Karbon Aktif Batang Kelapa Dan Bambu Terhadap Kandungan Tanin Seduhan Daun Stevia Dengan Metode Spektrofotometer Uv-Vis,” J. Ilm. Pharm., Vol. 10, 2023.
W. Amrinola, S. Widowati, And P. Hariyadi, “Metode Pembuatan Sorgum Sosoh Rendah Tanin Pada Pembuatan Nasi Sorgum (Sorghum Bicolor L) Instan,” Comtech Comput. Math. Eng. Appl., Vol. 6, No. 1, P. 9, 2015, Doi: 10.21512/Comtech.V6i1.2280.
S. Widarti, “Pengaruh Laju Alir Terhadap Efisiensi Kolom Resin Penukar Kation Komersil Dan Adsorpsi Ion Logam Berbeda Muatan,” Sigma-Mu, Vol. 7, No. 1, Pp. 1–6, 1970, Doi: 10.35313/Sigmamu.V7i1.833.
Efsa Panel On Food Additives And Nutrient Sources Added To Food (Ans), “Scientific Opinion On The Safety Of Steviol Glycosides For The Proposed Uses As A Food Additive,” Eur. Food Saf. Auth. (Efsa), Parma, Italy, Vol. 8, No. 4, P. 1537, 2010, Doi: 10.2903/J.Efsa.2010.1537.Available.
L. G. C. P. Rhl Vidal, “Study Of Adsorption Isotherms And Equilibrium For Removal Of Tannins From Cashew Apple Juice With Ion Exchange Resin,” J Chem Eng Catal, Vol. 1, Pp. 1–16, 2021.
H. Wuryantoro And W. Hadi Susanto, “Penyusunan Standard Operating Procedures Industri Rumah Tangga Pangan Pemanis Alami Instan Sari Stevia (Stevia Rebaudiana),” J. Pangan Dan Agroindustri, Vol. 2, No. 3, Pp. 76–87, 2014.
A. Widyasanti, K. Agnes, And N. Sarifah, “Pengaruh Rasio Bahan-Pelarut Dan Lama Terhadap Karakteristik Fisikokimia Ekstrak Cair Daun Stevia (Stevia Rebaudiana Bertoni),” J. Agroindustri, Vol. 12, No. 2, Pp. 86–95, 2022, Doi: 10.31186/J.Agroind.12.2.86-95.
K. C. Chandran And C. C. Kavitha Chandran, “Quantitative Estimation Of Total Phenolic, Flavonoids, Tannin And Chlorophyll Content Of Leaves Of Strobilanthes Kunthiana (Neelakurinji),” J. Med. Plants Stud. Med. Plants Stud., Vol. 4, No. 4, Pp. 282–286, 2016.
L. S. Magwaza And U. L. Opara, “Analytical Methods For Determination Of Sugars And Sweetness Of Horticultural Products-A Review,” Sci. Hortic. (Amsterdam)., Vol. 184, Pp. 179–192, 2015, Doi: 10.1016/J.Scienta.2015.01.001.
Fadila Kamilia Azzahra, I. Hidayati1, And H. Faizah1, “Pengaruh Penggunaan Media Tanam Terhadap Brix Dan Uji Organoleptik Tanaman Mentimun Jepang (Cucumis Sativus Var. Japonese) Dengan Hidroponik Sistem Drip,” Vol. 13, No. 2, Pp. 104–112, 2024.
D. I. A. Raharjo Hyacinta Trixie, “Efektivitas Ekstrak Etanol Tempe Koro Benguk (Mucuna Pruriens L.) Sebagai Chelating Agent Logam Berat Kadmium,” J. Ilm. Cendekia Eksakta, no. 2008, pp. 59–64, 2010.