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Abstract
Empirically, Inggu leaves (Ruta angustifolia L.) has potential to be used as raw material of traditional medicine because it contains phytochemical substances. The main organ most widely used as a traditional medicine is its leaves. Phytochemical compounds contained in the leaves of inggu include quercetin, tannin and saponins. Quercetin is a class of flavonol compounds (part of flavonoids). Quercetin has the ability to prevent the oxidation process from low density lipoprotein (LDL) by capturing free radicals and inhibiting transition metals, so that quercetin is believed to protect the body from various degerative diseases. While flavonoids are compounds consisting of 15 carbon atoms that
act as plant pigments. The function of flavonoids is to protect the cell structure, increase the effectiveness of vitamin C, antiinflammatory and as an antibiotic. While saponin and tannin are a group of active compounds of plants that have a sense of bitter and have antibacterial activity. This study was aim to determine the number of quercetin, tannins and saponins contained in the inggu’s leaves. Preparation of inggu leaf sample was done by maceration extraction technique used 96% ethanol solvent. Analysis of tannin and quercetin levels was determined by UV-Visible Spectrophotometry at 725 nm wavelength (λ).While the analysis of saponin content using TLC Scanner at 301 nm wavelength (λ). The results showed that content of quercetin was 1.67%; saponins 2.13% and tannins 7.04%.
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References
Akiyama, H. F., K. Iwatsuki, T. 2001. Antibacterial Action Of Several Tennis Agains Staphylococcus aureus. Journal of Antimicrobial Chemoterapy. Vol. 48: 487-91.
Ajizah, A. 2004. Sensitivitas Salmonella Typhimurium Terhadap Ekstrak Daun Psidium Guajava L. Bioscientie. Vol. 1 NO.1: 31-8.
Arief Hariana. 2013. 262 Tumbuhan Obat dan Khasiatnya. Jakarta: Penebar Swadaya.
Bernasconi, G.1995. Teknologi kimia I. Penerjemah; Handojo. L. Jakarta: PT. Prandya Paramitha.
Budisma. 2015. Jenis-Jenis Steroid dan Fungsinya. Diakses tanggal 2 Februari. http://budisma.net/2015/02/jenis-jenis-steroid-dan-fungsinya.html.
De padua. 1999. Senyawa Kimia. Diakses tanggal 2 Februari. http://www.tempo.co.id/medica/arsip/122002/art-3.htm.
Fania Putri Luhurningtyas. 2013. Aktivitas Larvasida Fraksi Non Polar Ekstrak Etanol Daun Inggu Terhadap Larva Nyamuk Anopheles aconitus dan Anopheles maculatus Beserta Profil Kromatografinya. Surakarta: UMS press. Diakses tanggal 20 Mei 2017. http://repository.ums.ac.id.
Franswort, N. R. 1996. Biological and Phytochemical Screenings of Plant. Journal of Pharmacy Science. Vol: 55 (3): 225-265.
Harborne, J.B.1996. Metode Fitokimia. Bandung: ITB press.
Kristiana, Maryani, dan Herti. 2008. Khasiat dan Manfaat Rosela. Jakarta: PT Agro Media Pustaka.
Markham, K.R.1998. Cara Mengidentifikasi Flavanoid. Bandung: ITB press.
Nurachman, Z. 2002. Artoindonesianin Untuk Antitumor.http.www.chem-istrri.
Mirna Lumbessy, dkk. 2015. Uji Total Flavonoid Pada Beberapa Tanaman Obat Tradisonal Di Desa Waitina Kecamatan Mangoli Timur Kabupaten Kepulauan Sula Provinsi Maluku Utara. JURNAL MIPA UNSRAT ONLINE 2 (1) 50-55. Diakses tanggal 20 Mei 2017.
Robinson, T. 1991.Kandungan Organik Tumbuhan Tingkat Tinggi, diterjemahkan oleh Prof. Dr. Kosasih Padmawinata. Bandung: ITB Press.
Sinaga, E. 2009. Mengenal dan Memanfaatkan Tumbuhan Obat untuk Pemeliharaan Kesehatan Sehari-Hari. Pusat Penelitian dan Pengembangan Tumbuhan Obat Universitas Nasional.Jakarta
Windy. 2013. The Antibacterial Activities Of The Extraxts Isolated From The Aruda Leaf (Ruta Angustifolia) Digested In Polar Semipolar Dan Nonpolar Solvent. Journal of applied chemistry science vol 2. No. 1. Deptartment of chemistry faculty of science and enginerring Nusa Cendana University.