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Latar belakang: Tingginya paparan radikal bebas saat ini berdampak negatif pada kesehatan dan estetika kulit, terutama kulit wajah, karena langsung terpapar dengan lingkungan tanpa adanya pelindung seperti bagian tubuh lainnya. Tubuh manusia membutuhkan antioksidan dalam jumlah yang cukup agar dapat meredam dampak negatif dari radikal bebas. Eucheuma cottoniidan Citrus lemon L memiliki senyawa bioaktif yang berpotensi sebagai antioksidan.
Tujuan: Penelitian ini dilakukan untuk mengetahui aktivitas antioksidan kombinasi ekstrak metanol Eucheuma cottoniiSumbawa (EMECS) dan ekstrak metanol Citrus lemon.L impor (EMCLI).
Metode: Pengujian aktivitas antioksidan menggunakan metode DPPH dengan parameter peredaman warna DPPH dan IC50.
Hasil: Ekstrak metanol tunggal ECS, CLI, dan kombinasi EMECS : EMCLI (1:1; 2:1; 1:2) memiliki aktivitas antioksidan yang ditunjukkan dengan memudarnya warna larutan DPPH, dan IC50 ekstrak tunggal ECS dan CLI sebesar 942,438 ppm dan 472,335 ppm, sedangkan untuk kombinasi EMECS : EMCLI (1:1; 2:1; 1:2) memiliki nilai 436,607 ppm; 500,094 ppm; 345,514 ppm. Combination Index EMECS : EMCLI (1:1; 2:1; 1:2) berturut-turut sebesar 0,59630; 1,43246; 0,46942 yang menunjukkan efek sinergis; antagonis menengah; dan sinergis untuk kombinasi kedua ekstrak tersebut
Kesimpulan: Dari penelitian ini, kombinasi ektrak Eucheuma cottonii Sumbawa dan ekstrak metanol Citrus lemon. L impor memiliki aktivitas antioksidan, dengan 2 perbandingan memiliki efek sinergis (1:1, 1:2) dan 1 perbandingan dengan efek antagonis menengah (2:1).
Kata kunci : aktivitas antioksidan, Eucheuma cottonii,Citrus limon L, DPPH, IC50
Antioxidant activity of Sumbawa red seaweed (Eucheuma cottonii) extract and lemon peel (Citrus limon L) extract combination
AbstractBackground: High exposure of free radicals have negative impact on the health and aesthetics of the skin, especially facial skin, especially when directly exposed without any protection. The human body needs antioxidants in sufficient quantities to reduce the negative effects of free radicals. Eucheuma cottonii and Citrus limon L have potential bioactive compounds as antioxidants.
Objective: This study was conducted to determine the antioxidant activity of combination Eucheuma cottonii Sumbawa methanol extract and Citrus limon peel methanol extract.
Method: Red seaweed and lemon peel were extracted using maceration method with methanol as solvent. Antioxidant activity test used DPPH method with DPPH color reduction and IC50 parameters.
Results: Methanol extract of EC, CLI, and their combination have antioxidant activity as indicated by the fading color of DPPH solution. The highest IC50 was obtained by methanol extract of lemon peel, and the lowest was achieved by combination 1:2. Combination Index of combination both extract have synergistic effect for 1:1, and 1:2, and intermediate antagonist for 2:1.
Conclusion:The combination of Sumbawa Eucheuma cottonii extract and Citrus limon L peel extract has antioxidant activity, with a synergistic effect (1:1, 1:2) and intermediate antagonistic effects (2: 1).
Keywords : antioxidant activity, Eucheuma cottonii L, Citrus limon L, DPPH, IC50
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