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cover JIF 18(1) 2022
Submitted
April 22, 2021
Accepted
February 26, 2022
Published
July 31, 2022
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Abstract

Background: Cancer is one of the main causes of death in the world for which no medicine has been found to date. One of the potential plants is Blumea balsamiferia.
Objective: This study aimed to determine the cytotoxic effect of B. balsamifera leaf extract and fractions.
Methods: The test was carried out using the Brine Shrimp Lethality Test (BSLT) on 48-hour-old shrimp (Artemia salina Leach) larvae. The concentrations of the test sample used were 10, 100, and 1000 ppm. Meanwhile, the negative control group was DMSO. Observations were made for 24 hours on larva deaths. The LC50 value was obtained using the probit analysis.
Results: The ethyl acetate fraction and ethanol extract were in the highly toxic category with LC50 values of 11.03 ppm and 20.28 ppm, while the n-hexane fraction and water fraction were in the toxic range with LC50 values of 67.82 ppm and 448.56 ppm, respectively.
Conclusion: The extract and fractions of B. balsamifera leaves have a cytotoxic effect.
Keywords: Brine shrimp lethality test, cytotoxic, Blumea balsamifera leaf


Intisari


Latar belakang: Kanker merupakan salah satu penyebab utama kematian di dunia yang belum ditemukan obatnya hingga sekarang. Salah satu tanaman yang berpotensi yaitu tumbuhan sembung (Blumea balsamiferia).
Tujuan: Penelitian ini untuk mengetahui efek sitotoksik ekstrak dan fraksi daun sembung.
Metode: Pengujian dilakukan dengan metode Brine Shrimp Lethality Test (BSLT) menggunakan larva udang (Artemia salina Leach) yang berumur 48 jam. Konsentrasi sampel uji yang yang digunakan 10, 100, dan 1000 ppm. Kontrol negatif menggunakan DMSO. Pengamatan dilakukan selama 24 jam pada kematian larva. Nilai LC50 diperoleh menggunakan analisis probit.
Hasil: Fraksi etil asetat dan ekstrak etanol berada dalam kategori sangat toksik dengan nilai LC50 11,03 ppm dan 20,28 ppm sedangkan fraksi n-heksan dan fraksi sisa air berada pada rentang toksik dengan nilai LC50 67,82 ppm dan 448, 56 ppm.
Kesimpulan: Ekstrak daun sembung memiliki efek sitotoksik.
Kata kunci: Brine shrimp lethality test, daun sembung (Blumea balsamifera), sitotoksik

Keywords

Brine shrimp lethality test cytotoxic Blumea balsamifera leaf

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Author Biography

Azimatur Rahmi, Universitas Mohammad Natsir Bukittinggi

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References

  1. Arel, A., Wardi, E. S., & Oktaviani, Y. (2018). Profil Metabolit Sekunder Ekstrak Daun Berenuk (Crescentia cujete L.) dan Uji Sitotoksik dengan Metode Brine Shrimp Lethality Test. Jurnal Katalisator, 3(2), 82-88. doi:http://doi.org/10.22216/jk.v3i2.3165
  2. Dona, R., Frimayanti, N., Ikhtiarudin, I., Iskandar, B., Maulana, F., & Silalahi, N. T. (2019). Studi In Silico, Sintesis, dan Uji Sitotoksik Senyawa P-Metoksi Kalkon Terhadap Sel Kanker Payudara MCF-7. Jurnal Farmasi Sains & Klinis, 6(3), 243-249. doi:https://doi.org/10.25077/jsfk.6.3.243-249.2019
  3. Eriadi, A., Uthia, R., & Novita, R. (2017). Pengaruh Pemberian Ekstrak Etanol Daun Sembung (Blumea balsamifera (L.) DC.) Terhadap Kadar Glukosa Darah dan Histopatologi Pankreas Mencit Putih Jantan yang Diinduksi Aloksan. Jurnal Farmasi Higea, 9(2), 127-139. doi:http://dx.doi.org/10.52689/higea.v9i2.168
  4. Fadhli, H., & Hasanah, S. b. U. (2019). Uji Sitotoksik Ekstrak Kulit Batang Tumbuhan Kangkang Katup (Bauhinia Semibifida Roxb) Dengan Metode Brine Shrimp Lethality Test (BSLT). Scientia: Jurnal Farmasi dan Kesehatan, 9(2), 141-145. doi:10.36434/SCIENTIA.V9I2.211
  5. Fadhli, H., Nurdin, A. N., & Octaviani, M. (2019). Potensi Antioksidan dari Ekstrak Kulit Batang Bauhinia semibifida Roxb. Jurnal Ilmiah Ibnu Sina, 4(1), 77-87. doi:https://doi.org/10.36387/jiis.v4i1.257
  6. Haryanti, S., Widayanti, E., & Widiyastuti, Y. (2017). Aktivitas Sitotoksik Ekstrak Air dan Etanol Kulit Manggis (Garcinia mangostana Linn.) pada beberapa Model Sel Kanker. Jurnal Tumbuhan Obat Indonesia, 10(1), 1-9. doi:10.22435/toi.v10i1.6742.1-9
  7. Juniarti, J., Osmeli, D., & Yuhernita, Y. (2009). Kandungan Senyawa Kimia, Uji Toksisitas (Brine Shrimp Lethality Test) Dan Antioksidan (1, 1-diphenyl-2-pikrilhydrazyl) Dari Ekstrak Daun Saga (Abrus precatorius L.). Makara, Sains, 13(1), 50-54. doi:https://doi/org/10.7454/mss.v13i1.378
  8. Marwati, M., Anggriani, A., Burhan, A., Awaluddin, A., Nur, S., Dharmayanti, R., Tiboyong, M. D. (2021). Antioxidant Activity and Cytotoxicity Against WiDR Cell and Vero Cell of The Karamunting (Rhonomyrtus tomentosa L.) Leaves Ethanol Extract. Indonesian Journal of Pharmaceutical Science and Technology, 8(3), 111-117. doi:https://doi.org/10.24198/ijpst.v8i3.26769
  9. Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E., & McLaughlin, J. L. (1982). Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. Planta Medica, 45(5), 31-34. doi:10.1055/s-2007-971236
  10. Norikura, T., Kojima-Yuasa, A., Shimizu, M., Huang, X., Xu, S., Kametani, S., Matsui-Yuasa, I. (2008). Mechanism of Growth Inhibitory Effect of Blumea balsamifera Extract in Hepatocellular Carcinoma. Bioscience, Biotechnology, and Biochemistry, 72(5), 1183-1189. doi:https://doi.org/10.1271/bbb.70586
  11. Ntungwe N, E., Dominguez-Martin, E. M., Roberto, A., Tavares, J., Isca, V., Pereira, P., Rijo, P. (2020). Artemia Species: An Important Tool to Screen General Toxicity Samples. Current Pharmaceutical Design, 26(24), 2892-2908. doi:10.2174/1381612826666200406083035
  12. Pang, Y., Wang, D., Fan, Z., Chen, X., Yu, F., Hu, X., Yuan, L. (2014). Blumea balsamifera—A Phytochemical and Pharmacological Review. Molecules, 19(7), 9453-9477. doi:10.3390/molecules19079453
  13. Rahmi, A., Afriani, T., Hevira, L., & Widiawati, W. (2021b). Uji Aktivitas Antioksidan dan Fenolik Total Fraksi Etil Asetat Daun Sembung (Blumea balsamifera (L.) DC). Jurnal Riset Kimia, 12(2). doi:https://doi.org/10.25077/jrk.v12i2.383
  14. Rahmi, A., Afriani, T., & Sari, L. P. (2021a). Uji Aktivitas Antipiretik Ekstrak Etanol Daun Sembung (Blumea balsamifera) Secara In Vivo terhadap Mencit Putih Jantan (Mus musculus). Majalah Farmasi dan Farmakologi, 25(1), 7-10. doi:https://doi.org/10.20956/mff.v25i1.11961
  15. Rohmah, R. N., Ratnaningtyas, N. I., & Asnani, A. (2014). Kajian Toksisitas dari Tubuh Buah Ganoderma lucidum dengan Metode Brine Shrimp Lethality Test (BSLT). Scripta Biologica, 1(1), 30-32. doi:10.20884/1.sb.2014.1.1.22
  16. Sumihe, G., Runtuwene, M. R. J., & Rorong, J. A. (2014). Analisis Fitokimia dan Penentuan Nilai LC50 Ekstrak Metanol Daun Liwas. Jurnal Ilmiah Sains, 14(2), 125-128. doi:https://doi.org/10.35799/jis.14.2.2014.6070
  17. Susanty, A., Dachriyanus, D., Yanwirasti, Y., Wahyuni, F. S., Fadhli, H., & Aswan, P. A. (2018). Aktivitas Sitotoksik Ekstrak Etil Asetat Daun Tampa Badak (Voacanga foetida (Bl.) K. Schum) pada Kanker Kolon HTB-38. Jurnal Sains Farmasi & Klinis, 5(2), 142-146. doi:https://doi.org/10.25077/jsfk.5.2.142-146.2018
  18. Widhiantara, I. G., Permatasari, A. A. A. P., Siswanto, F. M., & Dewi, N. P. E. S. (2018). Ekstrak Daun Sembung (Blumea balsamifera) Memperbaiki Histologi Testis Tikus Wistar yang diinduksi Pakan Tinggi Lemak. Jurnal Bioteknologi & Biosains Indonesia, 5(2), 111-118. doi:https://doi.org/10.29122/jbbi.v5i2.2868
  19. Widiyastuti, Y., Sholikhah, I. Y. M., & Haryanti, S. (2019). Efek Sitotoksik Formula Jamu Daun Sirsak, Buah Takokak, dan Umbi Bidara Upas Terhadap Sel Kanker Payudara Mcf-7. Jurnal Kefarmasian Indonesia, 9(2), 140-149. doi:https://doi.org/10.22435/jki.v9i2.1049
  20. Zuraida, Z. (2018). Analisis Toksisitas Beberapa Tumbuhan Hutan dengan Metode Brine Shrimp Lethality Test (BSLT). Jurnal Penelitian Hasil Hutan, 36(3), 239-246. doi:https://doi.org/10.20886/jphh.2018.36.3.239-246