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Abstract
Background: UV-A radiation induct disorder to the skin called photoaging. It requires treatment with antioxidant agent. Superoxide dismutase (SOD) is the first line antioxidant in the treatment of free radicals.
Objective: This study aimed to find out anti-photoaging activities of SOD extract of corn (Zea mays L.) kernels on radiated UV-A rays 3T3 fibroblast cells.
Method: SOD extraction of corn kernels was done by centrifugation, precipitation and dialysis method. The activity of SOD extract in corn kernels was measured by WST-1 method. Anti-photoaging activity test of SOD extract in corn kernels was done on radiated UV-A rays 3T3 fibroblast cells with parameters of cell viability. Anti-photoaging activity test using cell control, negative, sample and positive control.
Results: SOD activity showed that the highest percent inhibition value was SOD extract of corn kernels in precipitation of ammonium sulphate concentration was 60-90% with inhibition percentage was 65.50%. The result of anti-photoaging activity SOD extract of corn kernels showed significant difference in the value of percent cell viability in fibroblast cells between the extract group and the negative control group with the highest percent cell viability in SOD extract corn kernels with the concentration was 100 mg/mL, namely 142.31%.
Conclusion: SOD extract from corn kernels has proven had antioxidant activity that could be used as anti-photoaging through fibroblast cell reparation mechanism.
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References
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- Baharvand, H. (2015). Stem-Cell Nanoengineering New Jersey: Wiley Blackwell
- Baran, S. J., & K, D. D. (2010). Teori Komunikasi Massa: Dasar, Pergolakan dan Masa Depan Jakarta: Salemba Humanika
- Baum, J. A., Chandlee, J. M., & Scandalios, J. G. (1983). Purification and Partial Characterization of a Genetically-Defined Superoxide Dismutase (SOD-1) Associated with Maize Chloroplasts. Plant Physiol, 73(1), 31-35. https://doi.org/10.1104/pp.73.1.31
- CCRC. (2009). Prosedur tetap Uji Sitotoksik Metode MTT Yogyakarta: Cancer Chemoprevention Research Center Farmasi UGM Yogyakarta.6–9
- Cheong, Y., Kim, C., Kim, M. B., & Hwang, J. K. (2018). The anti-photoaging and moisturizing effects of Bouea macrophylla extract in UVB-irradiated hairless mice. Food Sci Biotechnol, 27(1), 147-157. https://doi.org/10.1007/s10068-017-0276-y
- Choi, S. H., Choi, S. I., Jung, T. D., Cho, B. Y., Lee, J. H., Kim, S. H., Yoon, S. A., Ham, Y. M., Yoon, W. J., Cho, J. H., & Lee, O. H. (2017). Anti-Photoaging Effect of Jeju Putgyul (Unripe Citrus) Extracts on Human Dermal Fibroblasts and Ultraviolet B-induced Hairless Mouse Skin. Int J Mol Sci, 18(10). https://doi.org/10.3390/ijms18102052
- Damayanti, N. M., & Ratnayanti, I. D. (2013). Therapeutic of Skin Aging with Carbon Dioxide Laser Skin Resurfacing And Combination With Air Cooling. E-Jurnal Medika Udayana, 2054-2061. https://ojs.unud.ac.id/index.php/eum/article/view/7340
- Debacq-Chainiaux, F., Leduc, C., Verbeke, A., & Toussaint, O. (2012). UV, stress and aging. Dermatoendocrinol, 4(3), 236-240. https://doi.org/10.4161/derm.23652
- Ebadi, P., & Fazeli, M. (2017). Anti-Photoaging Potential of Propolis Extract in UVB-Irradiated Human Dermal Fibroblast Through Increasing The Expression of FOXO3A and NGF Genes, Biomed Pharmacother. Biomedicine & Pharmacotherapy, 95, 47-54. https://doi.org/https://doi.org/10.1016/j.biopha.2017.08.019
- Indrayati, A., Asyarie, S., Suciati, T., & Retnoningrum, D. S. (2016). Pengaruh Superoksida Dismutase Rekombinan Staphylococcus equorum terhadap Viabilitas Sel dan Deposisi Kolagen Pada Sel Fibroblas 3T3 Yang Dipaparkan UVA. Jurnal Farmasi Indonesia, 13 (1), 34-40.
- Kartawiguna, E. (2011). Dermatoheliosis. Retrieved September 2019 from https://univmed.org/wp-content/uploads/2011/02/vol.18%20no.1
- Niyomploy, P., Boonsombat, R., Karnchanatat, A., & Sangvanich, P. (2014). A Superoxide Dismutase Purified From The Roots From Stemona tuberosa. Preparative Biochemistry & Biotechnology, 44(7), 663-679. https://doi.org/10.1080/10826068.2013.868356
- Park, M. A., Sim, M. J., & Kim, Y. C. (2017). Anti-Photoaging Effects of Angelica acutiloba Root Ethanol Extract in Human Dermal Fibroblasts. Toxicol Res, 33(2), 125-134. https://doi.org/10.5487/tr.2017.33.2.125
- Pratiwi, S., & Husni, P. (2017). Potensi Penggunaan Fitokonstituen Tanaman Indonesia sebagai Bahan Aktif Tabir Surya: Review.
- Rizqi, J., & Amestiasih, T. (2020). Pengaruh Kombinasi Madu dan Lidah Buaya (Aloe Vera) Terhadap Proliferrasi pada Sel Line Fibroblast NIH3T3: Studi In Vitro. Prosiding Seminar Nasional Multi Disiplin Ilmu UNRIYO, 2(1), 535-541.
- Sharma, S., Bahuguna, S., Nameet, K., & Chaudhary, N. (2014). Biochemical aspects of superoxide dismutase isolated from Amaranthus spinosus: A Therapeutically Important Plant. International Journal of Genetic Engineering and Biotechnology, 0974-3073]. 5, 35-42.
- Svobodova, A., Walterova, D., & Vostalova, J. (2006). Ultraviolet Light Induced Alteration to The Skin. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 150(1), 25-38. https://doi.org/10.5507/bp.2006.003
- Wahyu, W., Ratu, S., Rymond, R., & Marlinda, S. (2005). Penapisan Aktivitas Superoksida Dismutase Pada Berbagai Tanaman. Maranatha Journal of Medicine and Health, 5(1).
- Yong, H. J., & Ahn, J. J. (2018). Antioxidant and Skin Protection Effect of Morin Upon UV-A Exposure. Biomedical Dermatology. Biomedical Dermatology, 2(1), 12. https://doi.org/10.1186/s41702-018-0026-7
References
Amaro-Ortiz, A., Yan, B., & D'Orazio, J. A. (2014). Ultraviolet Radiation, Aging and the Skin: Prevention of Damage by Topical cAMP Manipulation. Molecules, 19(5), 6202-6219. https://doi.org/10.3390/molecules19056202
Baharvand, H. (2015). Stem-Cell Nanoengineering New Jersey: Wiley Blackwell
Baran, S. J., & K, D. D. (2010). Teori Komunikasi Massa: Dasar, Pergolakan dan Masa Depan Jakarta: Salemba Humanika
Baum, J. A., Chandlee, J. M., & Scandalios, J. G. (1983). Purification and Partial Characterization of a Genetically-Defined Superoxide Dismutase (SOD-1) Associated with Maize Chloroplasts. Plant Physiol, 73(1), 31-35. https://doi.org/10.1104/pp.73.1.31
CCRC. (2009). Prosedur tetap Uji Sitotoksik Metode MTT Yogyakarta: Cancer Chemoprevention Research Center Farmasi UGM Yogyakarta.6–9
Cheong, Y., Kim, C., Kim, M. B., & Hwang, J. K. (2018). The anti-photoaging and moisturizing effects of Bouea macrophylla extract in UVB-irradiated hairless mice. Food Sci Biotechnol, 27(1), 147-157. https://doi.org/10.1007/s10068-017-0276-y
Choi, S. H., Choi, S. I., Jung, T. D., Cho, B. Y., Lee, J. H., Kim, S. H., Yoon, S. A., Ham, Y. M., Yoon, W. J., Cho, J. H., & Lee, O. H. (2017). Anti-Photoaging Effect of Jeju Putgyul (Unripe Citrus) Extracts on Human Dermal Fibroblasts and Ultraviolet B-induced Hairless Mouse Skin. Int J Mol Sci, 18(10). https://doi.org/10.3390/ijms18102052
Damayanti, N. M., & Ratnayanti, I. D. (2013). Therapeutic of Skin Aging with Carbon Dioxide Laser Skin Resurfacing And Combination With Air Cooling. E-Jurnal Medika Udayana, 2054-2061. https://ojs.unud.ac.id/index.php/eum/article/view/7340
Debacq-Chainiaux, F., Leduc, C., Verbeke, A., & Toussaint, O. (2012). UV, stress and aging. Dermatoendocrinol, 4(3), 236-240. https://doi.org/10.4161/derm.23652
Ebadi, P., & Fazeli, M. (2017). Anti-Photoaging Potential of Propolis Extract in UVB-Irradiated Human Dermal Fibroblast Through Increasing The Expression of FOXO3A and NGF Genes, Biomed Pharmacother. Biomedicine & Pharmacotherapy, 95, 47-54. https://doi.org/https://doi.org/10.1016/j.biopha.2017.08.019
Indrayati, A., Asyarie, S., Suciati, T., & Retnoningrum, D. S. (2016). Pengaruh Superoksida Dismutase Rekombinan Staphylococcus equorum terhadap Viabilitas Sel dan Deposisi Kolagen Pada Sel Fibroblas 3T3 Yang Dipaparkan UVA. Jurnal Farmasi Indonesia, 13 (1), 34-40.
Kartawiguna, E. (2011). Dermatoheliosis. Retrieved September 2019 from https://univmed.org/wp-content/uploads/2011/02/vol.18%20no.1
Niyomploy, P., Boonsombat, R., Karnchanatat, A., & Sangvanich, P. (2014). A Superoxide Dismutase Purified From The Roots From Stemona tuberosa. Preparative Biochemistry & Biotechnology, 44(7), 663-679. https://doi.org/10.1080/10826068.2013.868356
Park, M. A., Sim, M. J., & Kim, Y. C. (2017). Anti-Photoaging Effects of Angelica acutiloba Root Ethanol Extract in Human Dermal Fibroblasts. Toxicol Res, 33(2), 125-134. https://doi.org/10.5487/tr.2017.33.2.125
Pratiwi, S., & Husni, P. (2017). Potensi Penggunaan Fitokonstituen Tanaman Indonesia sebagai Bahan Aktif Tabir Surya: Review.
Rizqi, J., & Amestiasih, T. (2020). Pengaruh Kombinasi Madu dan Lidah Buaya (Aloe Vera) Terhadap Proliferrasi pada Sel Line Fibroblast NIH3T3: Studi In Vitro. Prosiding Seminar Nasional Multi Disiplin Ilmu UNRIYO, 2(1), 535-541.
Sharma, S., Bahuguna, S., Nameet, K., & Chaudhary, N. (2014). Biochemical aspects of superoxide dismutase isolated from Amaranthus spinosus: A Therapeutically Important Plant. International Journal of Genetic Engineering and Biotechnology, 0974-3073]. 5, 35-42.
Svobodova, A., Walterova, D., & Vostalova, J. (2006). Ultraviolet Light Induced Alteration to The Skin. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 150(1), 25-38. https://doi.org/10.5507/bp.2006.003
Wahyu, W., Ratu, S., Rymond, R., & Marlinda, S. (2005). Penapisan Aktivitas Superoksida Dismutase Pada Berbagai Tanaman. Maranatha Journal of Medicine and Health, 5(1).
Yong, H. J., & Ahn, J. J. (2018). Antioxidant and Skin Protection Effect of Morin Upon UV-A Exposure. Biomedical Dermatology. Biomedical Dermatology, 2(1), 12. https://doi.org/10.1186/s41702-018-0026-7