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Submitted
August 10, 2022
Accepted
July 6, 2023
Published
July 31, 2023
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Abstract

Abstract
Background: The COVID-19 pandemic had caused the consumption of vitamins in the community to increase with the aim of maintaining immunity in the body.
Objective: This study aims to develop an analysis method with a UV-Vis spectrophotometer, which is considered an alternative analysis method that is easier, more practical, and cheaper.
Method: Methods for analyzing alpha-tocopherol acetate are validated through specificity tests, linearity measurements, precision tests, accuracy tests, determination of detection limits, determination of quantitation limits, and determination of range using ethanol p.a solvent and UV-Vis Spectrophotometer instruments. Then, examine the alpha tocopherol acetate content of vitamin E dietary supplements.
Results: The specificity test showed that matrix had no significant effect on the analysis, the linearity test met the requirements with a concentration range of 20–60 ug/mL (r = 0.9991), the accuracy test was 99.897-100.315%, intraday and interday precision tests were obtained with %RSD less than 2%, the LOD value was 1.859 ug/mL and the LOQ value was 5.635 ug/mL. The determination of levels of vitamin E in dietary supplements showed levels for brand A of 100.508% and brand B of 100.089%.
Conclusion: The analytical method developed had met the validation requirements, and the levels of vitamin E food supplements had met the USP requirements.
Keywords: method validation, UV-Vis spectrophotometer, alpha tocopherol acetate


Intisari
Latar belakang: Pandemi COVID-19 menyebabkan penggunaan vitamin di masyarakat meningkat dengan tujuan agar imunitas pada tubuh tetap terjaga.
Tujuan: Mengembangkan dan memvalidasi metode analisis vitamin E dengan tujuan uji kualitatif dan kuantitatif menggunakan spektrofotometer UV-Vis yang dinilai sebagai metode analisis alternatif yang lebih mudah, praktis, dan murah.
Metode: Validasi metode yang dilakukan untuk analisis senyawa alfa tokoferol asetat meliputi uji spesifisitas, pengukuran linieritas, uji presisi, uji akurasi, penentuan limit deteksi, penentuan limit kuantifikasi, dan penentuan rentang menggunakan pelarut etanol p.a. dan alat spektrofotometer UV-Vis. Metode yang telah memenuhi kriteria validasi kemudian digunakan untuk pengecekan kadar kandungan alfa tokoferol asetat dalam suplemen vitamin E.
Hasil: Penelitian ini memberikan hasil yaitu matriks tidak teranalisis pada uji spesifisitas, uji linieritas telah memenuhi syarat dengan rentang konsentrasi 20–60 ug/mL (r2= 0,9991), uji akurasi 99,897-100,315%, uji presisi interday dan intraday didapatkan hasil %RSD kurang dari 2%, nilai LOD 1,859 ug/mL dan nilai LOQ 5,635 ug/mL. Penetapan kadar sediaan vitamin E telah memenuhi ketentuan yaitu 100,508% dan 100,089%.
Kesimpulan: Metode yang dikembangkan telah memenuhi ketentuan validitas dan kadar vitamin E pada sampel sesuai dengan ketentuan USP.
Kata kunci: validasi metode, spektrofotometer UV-Vis, alfa tokoferol asetat

Keywords

method validation UV-Vis spectrophotometer alpha tocopherol acetate

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Author Biographies

Arif Al Iman, Program Studi Farmasi, Institut Teknologi Sumatera, Lampung, Indonesia

 

 

Winni Auli, Program Studi Farmasi, Institut Teknologi Sumatera, Lampung, Indonesia

 

 

Sukrasno, Sekolah Farmasi, Institut Teknologi Bandung, Jawa Barat, Indonesia

 

 

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