Article Sidebar

Submitted
November 12, 2024
Accepted
May 30, 2026
Published
May 31, 2026
Download
Statistic
Read Counter : 6

Downloads

Download data is not yet available.

Main Article Content

Abstract

Background: Kebiul seeds are an Indonesian medicinal plant with potential antioxidant and chemopreventive properties against cancer. The development, growth, and invasion of malignant cancer closely link to angiogenesis, which antioxidant effects can influence.
Objective: This study aims to evaluate the antioxidant activity of kebiul seeds based on the IC50 and their anti-angiogenesis effects.
Method: Extraction was performed using the maceration method using ethyl acetate, n-hexane, and ethanol. The anti-angiogenesis test was conducted using the chorioallantoic membrane (CAM) method, while the antioxidant activity was assessed using the diphenylpyrrolhydrazyl (DPPH) method.
Results: The finding revealed the antioxidant activity values, as indicated by the IC50, were 45.225 ppm for the ethanol extract (strong antioxidant), 86.190 ppm for the n-hexane extract (strong antioxidant), and 146 ppm for the ethyl acetate extract (medium antioxidant). Anti-angiogenesis tests were conducted across various solvents and concentrations. Significant anti-angiogenesis effects were observed at concentrations of 10, 50, and 1000 ppm. The n-hexane extract yielded average scores of 0.33, 0.66, and 1.3, indicating a good effect. The ethyl acetate extract had average scores of 0.33, 0.5, and 0.66, suggesting a weak effect, while the ethanol extract showed mean scores of 1.33, 1.66, and 2, indicating a strong effect.
Conclusion: Ethanol extract demonstrates the most potential as a chemopreventive agent.

Keywords

Antioxidant anti-angiogenesis CAM kebiul seeds

Article Details

License

Copyright (c) 2026 Septi Wulandari, Risky Hadi Wibowo, Ria Nurwidiyani, Risda Hayati , Nadia, Amey Putri

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors who publish in the Jurnal Ilmiah Farmasi agree to the following terms:

  1. Authors retain copyright and grant Jurnal Ilmiah Farmasi right of first publication with the work simultaneously licensed under a Creative Commons Attribution Licence that allows others to adapt (remix, transform, and build) upon the work non-commercially with an acknowledgement of the work's authorship and initial publication in Jurnal Ilmiah Farmasi.
  2. Authors are permitted to share (copy and redistribute) the journal's published version of the work non-commercially (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in Jurnal Ilmiah Farmasi.
Crossref
Scopus
Google Scholar
Europe PMC

References

  1. Aftrid, Z. H. V. I., & Cahyana, A. H. (2022). Synthesis of Carbazole Derivative Compound with the Mannich Reaction and Antioxidant Activity. AIP Conference Proceedings, 2563(1), 1–10. https://doi.org/10.1063/5.0104131
  2. Hafsah, L. (2022). GAMBARAN TINGKAT KECEMASAN PADA PASIEN KANKER YANG MENJALANI KEMOTERAPI DI RSUD Dr. M. YUNUS BENGKULU. Jurnal Vokasi Keperawatan (JVK), 5(1), 21–28. https://doi.org/10.33369/jvk.v5i1.22338
  3. Hashemzaei, M., Far, A. D., Yari, A., Heravi, R. E., Tabrizian, K., Taghdisi, S. M., Sadegh, S. E., Tsarouhas, K., Kouretas, D., Tzanakakis, G., Nikitovic, D., Anisimov, N. Y., Spandidos, D. A., Tsatsakis, A. M., & Rezaee, R. (2017). Anticancer and Apoptosis-Inducing Effects of Quercetin in Vitro and in Vivo. Oncology Reports, 38(2), 819–828. https://doi.org/10.3892/or.2017.5766
  4. Hasmila, I., Natsir, H., & Soekamto, N. H. (2019). Phytochemical Analysis and Antioxidant Activity of Soursop Leaf Extract (Annona muricata Linn.). Journal of Physics: Conference Series, 1341(3), 1–6. https://doi.org/10.1088/1742-6596/1341/3/032027
  5. Hassan, A., Akmal, Z., Khan, N., & Alparslan, Y. (2020). The Phytochemical Screening and Antioxidants Potential of Schoenoplectus triqueter L. Palla. Journal of Chemistry, 2020(1), 1–8. https://doi.org/10.1155/2020/3865139
  6. Jalil Sarghaleh, S., Alizadeh Behbahani, B., Hojjati, M., Vasiee, A., & Noshad, M. (2023). Evaluation of the Constituent Compounds, Antioxidant, Anticancer, and Antimicrobial Potential of Prangos ferulacea Plant Extract and its Effect on Listeria monocytogenes Virulence Gene Expression. Frontiers in Microbiology, 14(1), 1–14. https://doi.org/10.3389/fmicb.2023.1202228
  7. Jiamjariyatam, R., Phucharoenrak, P., Samosorn, S., Dolsophon, K., Lorliam, W., Krajangsang, S., & Tantayotai, P. (2023). Influence of Different Extraction Methods on the Changes in Bioactive Compound Composition and Antioxidant Properties of Solid-State Fermented Coffee Husk Extracts. Scientific World Journal, 2023(1), 1–8. https://doi.org/10.1155/2023/6698056
  8. Khater, M., Greco, F., & Osborn, H. M. I. (2020). Antiangiogenic Activity of Flavonoids: A Systematic Review and Meta-Analysis. Molecules, 25(20), 1–31. https://doi.org/10.3390/molecules25204712
  9. Li, B., Shao, H., Gao, L., Li, H., Sheng, H., & Zhu, L. (2022). Nano-Drug Co-Delivery System of Natural Active Ingredients and Chemotherapy Drugs for Cancer Treatment: A Review. Drug Delivery, 29(1), 2130–2161. https://doi.org/10.1080/10717544.2022.2094498
  10. Malviya, N., & Malviya, S. (2017). Bioassay guided fractionation-an emerging technique influence the isolation, identification and characterization of lead phytomolecules. International Journal of Hospital Pharmacy, January 2017. https://doi.org/10.28933/ijhp-2017-07-0901
  11. Mondal, A., Gandhi, A., Fimognari, C., Atanasov, A. G., & Bishayee, A. (2019). Alkaloids for Cancer Prevention and Therapy: Current Progress and Future Perspectives. European Journal of Pharmacology, 858(June), 172472. https://doi.org/10.1016/j.ejphar.2019.172472
  12. Natsir, H., Wahab, A. W., Budi, P., Dali, S., & Arif, A. R. (2019). Amino Acid and Mineral Composition of Moringa Oleivera Leaves Extract and its Bioactivity As Antioxidant. Journal of Physics: Conference Series, 1317(1), 1–9. https://doi.org/10.1088/1742-6596/1317/1/012030
  13. Vilkickyte, G., Raudone, L., & Petrikaite, V. (2020). Phenolic Fractions from V accinium vitis‐idaea L. nd Their Antioxidant and Anticancer Activities Assessment. Antioxidants, 9(12), 1–20. https://doi.org/10.3390/antiox9121261
  14. Warsi, & Sholichah, A. R. (2017). Phytochemical Screening and Antioxidant Activity Ethanolic Extract and Ethyl Acetate Fraction from Basil Leaf (Ocimum basilicum L.) by DPPH Radical Scavenging Method. IOP Conference Series: Materials Science and Engineering, 259(1), 1–11. https://doi.org/10.1088/1757-899X/259/1/012008