Main Article Content
Abstract
Background: Binahong plants are reportedly containing polyphenols, flavonoids, and steroid compounds. The content of polyphenols and flavonoids plays an important role as antioxidants. Research using the method of FRAP (Ferric Reducing Ability of Power) indicates that the antioxidant content of the binahongleaves extract is 3.68 mmol/100 g in dried leaves. This study offers new proof of antioxidant activity of all the aerial part of binahong.
Objective: This research aims to determine the total phenolic content from binahong extract and its ability as free radical scavenger using DPPH method.
Methods: The plant used in this research is every part of plants on the ground. The binahong extract is obtained by a maceration method with various solvents (hexane, chloroform, and methanol). Total phenolic content in the extract is spectrophotometrically determined with the Folin Ciocalteu reagent. Antioxidant activity assay is performed in vitro by the method of DPPH, with Gallic acid as a comparator. ES50 parameter is determined from the linear regression equation between the concentration of extracts and % scavenging.
Results: The results showed that the total phenolic content of hexane, chloroform and methanol extract of Binahong was 8.54 ± 0.49 GAE mg/g, 17.30 ± 0.47 GAE mg/g and 32.5 ± 1.11 GAE mg/g. Hexane extract, chloroform extract and methanol extract of Binahong have a free radical scavenger activity. The value of ES50 extracts of hexane, chloroform, methanol, and successive acid errors is 58.601 ± 2.533 µg/ml, 446.219 ± 2.268 µg/ml, 237.683 ± 13.373 µg/ml and 2.058 ± 0.002 µg/ml.
Conclusion: The capability of the three of binahong extract as a free radicals DPPH scavenger were weaker than gallic acid.Background: Binahong plants are reportedly containing polyphenols, flavonoids, and steroid compounds. The content of polyphenols and flavonoids plays an important role as antioxidants. Research using the method of FRAP (Ferric Reducing Ability of Power) indicates that the antioxidant content of the binahongleaves extract is 3.68 mmol/100 g in dried leaves. This study offers new proof of antioxidant activity of all the aerial part of binahong.
Objective: This research aims to determine the total phenolic content from binahong extract and its ability as free radical scavenger using DPPH method.
Methods: The plant used in this research is every part of plants on the ground. The binahong extract is obtained by a maceration method with various solvents (hexane, chloroform, and methanol). Total phenolic content in the extract is spectrophotometrically determined with the Folin Ciocalteu reagent. Antioxidant activity assay is performed in vitro by the method of DPPH, with Gallic acid as a comparator. ES50 parameter is determined from the linear regression equation between the concentration of extracts and % scavenging.
Results: The results showed that the total phenolic content of hexane, chloroform and methanol extract of Binahong was 8.54 ± 0.49 GAE mg/g, 17.30 ± 0.47 GAE mg/g and 32.5 ± 1.11 GAE mg/g. Hexane extract, chloroform extract and methanol extract of Binahong have a free radical scavenger activity. The value of ES50 extracts of hexane, chloroform, methanol, and successive acid errors is 58.601 ± 2.533 µg/ml, 446.219 ± 2.268 µg/ml, 237.683 ± 13.373 µg/ml and 2.058 ± 0.002 µg/ml.
Conclusion: The capability of the three of binahong extract as a free radicals DPPH scavenger were weaker than gallic acid.
Keywords
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References
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- Kabel AM. Free radicals and antioxidants: Role of enzymes and nutrition. World Journal Nutrition and Health. 2014;2(3):35–8.
- Hernani, Raharjo M. Tanaman obat berkhasiat. Jakarta Indonesia: Penebar Swadaya; 2005. 3, 56 p.
- Lobo V, Pathil A, Pathak A, Chandra N. Free radicals, antioxidants and functional Foods?: Impacton human health. Pharmacognosy Reviews. 2010;4(8):118–26.
- Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology. 2007 Jan 1 [cited 2019 Jul 16];39(1):44–84.
- Soong YY, Barlow PJ. Antioxidant activity and phenolic content of selected fruit seeds. Food Chemistry. 2004;88(3):411–7.
- Rahmawati F, Bintari SH. Studi aktivitas antibakteri sari daun binahong (Anredera cordifolia) terhadap pertumbuhan Bacillus cereus dan Salmonella enteritidis. Unnes Journal of Life Science. 2014;3(2):103–11.
- Astuti SM, Sakinah A.M M, Andayani B.M R, Risch A. Determination of saponin compound from Anredera cordifolia (Ten) Steenis plant (Binahong) to potential treatment for several diseases. Journal of Agricultural Science. 2011;3(4):224–32.
- Fidrianny I, Ruslan Wirasutisna K, Amanda P. Senyawa antioksidan dari ekstrak etil asetat daun Binahong (Anredera cordifolia (Ten.) Steenis) dari Babakan Ciparay, Bandung Selatan, Indonesia. Acta Pharmaceutica Indonesia. 2013;26(1):26–30.
- Parwati N, Napitupulu M, Diah A. Uji aktivitas antioksidan ekstrak daun binahong (Anredera Cordifolia (Tenore) Steenis) dengan 1,1-Difenil-2-Pikrilhidrazil (DPPH) menggunakan spektrofotometer UV-Vis. Jurnal Akademika Kimia. 2014;3(4):206–13.
- Philip M. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science of Technology. 2004;26(2):211–9.
- Alfian R, Susanti H. Penetapan kadar fenolik total ekstrak metanol kelopak bunga rosela merah (Hibbiscus sabdariffa Linn) dengan variasi tempat tumbuh. Pharmaciana. 2012;2(1):73–80.
- Djamil R, Wahyudi PS, Wahono S, Hanafi M. Antioxidant activity of flavonoid from Anredera Cordifolia (Ten) Steenis Leaves. International Research of Journal of Pharmacy. 2012;3(9):241–3.
- Puspita Sari E, Ningsih IY. Kapasitas antioksidan ekstrak buah salak (Salacca zalacca (Gaertn) Voss) varian gula pasir menggunakan metode penangkapan radikal DPPH. Pharmacy. 2016;13(01):116–26.
- Selawa W, Revolta M, Runtuwene J, Citraningtyas G, Studi P, Fmipa F, et al. Kandungan flavonoid dan kapasitas antioksidan total ekstrak etanol daun binahong, Pharmacon, 2013;2(01):18-23.
- Susanti H. In vitro antioxidant activity of lampes ( ocimum sanctum ) leaves and seeds ethanol extract using DPPH method. In: International Conference on Drug Development from Natural Resources. 2012. p. 269–74.
- Büyüktuncel E, Porgali E, Çolak C. Comparison of total phenolic content and total antioxidant activity in local red wines determined by spectrophotometric methods. Food and Nutrition Science. 2014;05(17):1660–7.
References
Surveswaran S, Cai Y-Z, Corke H, Sun M. Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chemistry.. 2007 Jan 1 [cited 2019 Jul 16];102(3):938–53.
Kabel AM. Free radicals and antioxidants: Role of enzymes and nutrition. World Journal Nutrition and Health. 2014;2(3):35–8.
Hernani, Raharjo M. Tanaman obat berkhasiat. Jakarta Indonesia: Penebar Swadaya; 2005. 3, 56 p.
Lobo V, Pathil A, Pathak A, Chandra N. Free radicals, antioxidants and functional Foods?: Impacton human health. Pharmacognosy Reviews. 2010;4(8):118–26.
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology. 2007 Jan 1 [cited 2019 Jul 16];39(1):44–84.
Soong YY, Barlow PJ. Antioxidant activity and phenolic content of selected fruit seeds. Food Chemistry. 2004;88(3):411–7.
Rahmawati F, Bintari SH. Studi aktivitas antibakteri sari daun binahong (Anredera cordifolia) terhadap pertumbuhan Bacillus cereus dan Salmonella enteritidis. Unnes Journal of Life Science. 2014;3(2):103–11.
Astuti SM, Sakinah A.M M, Andayani B.M R, Risch A. Determination of saponin compound from Anredera cordifolia (Ten) Steenis plant (Binahong) to potential treatment for several diseases. Journal of Agricultural Science. 2011;3(4):224–32.
Fidrianny I, Ruslan Wirasutisna K, Amanda P. Senyawa antioksidan dari ekstrak etil asetat daun Binahong (Anredera cordifolia (Ten.) Steenis) dari Babakan Ciparay, Bandung Selatan, Indonesia. Acta Pharmaceutica Indonesia. 2013;26(1):26–30.
Parwati N, Napitupulu M, Diah A. Uji aktivitas antioksidan ekstrak daun binahong (Anredera Cordifolia (Tenore) Steenis) dengan 1,1-Difenil-2-Pikrilhidrazil (DPPH) menggunakan spektrofotometer UV-Vis. Jurnal Akademika Kimia. 2014;3(4):206–13.
Philip M. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science of Technology. 2004;26(2):211–9.
Alfian R, Susanti H. Penetapan kadar fenolik total ekstrak metanol kelopak bunga rosela merah (Hibbiscus sabdariffa Linn) dengan variasi tempat tumbuh. Pharmaciana. 2012;2(1):73–80.
Djamil R, Wahyudi PS, Wahono S, Hanafi M. Antioxidant activity of flavonoid from Anredera Cordifolia (Ten) Steenis Leaves. International Research of Journal of Pharmacy. 2012;3(9):241–3.
Puspita Sari E, Ningsih IY. Kapasitas antioksidan ekstrak buah salak (Salacca zalacca (Gaertn) Voss) varian gula pasir menggunakan metode penangkapan radikal DPPH. Pharmacy. 2016;13(01):116–26.
Selawa W, Revolta M, Runtuwene J, Citraningtyas G, Studi P, Fmipa F, et al. Kandungan flavonoid dan kapasitas antioksidan total ekstrak etanol daun binahong, Pharmacon, 2013;2(01):18-23.
Susanti H. In vitro antioxidant activity of lampes ( ocimum sanctum ) leaves and seeds ethanol extract using DPPH method. In: International Conference on Drug Development from Natural Resources. 2012. p. 269–74.
Büyüktuncel E, Porgali E, Çolak C. Comparison of total phenolic content and total antioxidant activity in local red wines determined by spectrophotometric methods. Food and Nutrition Science. 2014;05(17):1660–7.