Article Sidebar

Submitted
January 29, 2019
Accepted
December 29, 2019
Published
December 30, 2019
Download
PDF EC (Bahasa Indonesia) Formulir Copyright Transfer (Bahasa Indonesia) (Bahasa Indonesia) (Bahasa Indonesia) (Bahasa Indonesia) (Bahasa Indonesia) (Bahasa Indonesia) (Bahasa Indonesia) (Bahasa Indonesia)
Statistic
Read Counter : 1076 Download : 1636

Main Article Content

Abstract

Background: Meniran (Phyllathus niruri) and pegagan (Centella asiatica) are well-known medicinal plants in Indonesia. Uses of meniran and pegagan as herbal medicines need to be examined for their subchronic activity to assure their safety.
Objective: This study aimed to evaluate subchronic effects of a combination of extracted meniran and pegagan on Wistar rats through haematological parameters (erythrocytes, haemoglobin, haematocrit, MCV, MCH, and MCHC).
Methods: Animal models used were 56 male and female Wistar rats and were divided randomly into 4 groups. Group 1 received CMC-Na 1% (control group). Group 2 received extracts of meniran and pegagan (50:50 mg/kgBW). Group 3 received extracts of meniran and pegagan (250:250 mg/kgBW). Group 4 received extracts of meniran and pegagan (1250:1250 mg/kgBW). Subchronic test of meniran and pegagan was conducted by providing treatments for the Wistar rats for 28 days. Their haematological substances were analysed statistically by using one way ANOVA and Kruskal-Wallis method with confidence interval of 95% and post-hoc.
Results: This study found that the haematological substances in male Wistar rats were normal and did not significantly change (p>0,05). It also showed that haemoglobin and haematocrit substances in female rats were normal and did not significantly change (p>0,05). Erythrocytes, MCV, MCH, and MCHC in female rats indicated significant change (p<0,05), but they were still in normal ranges.
Conclusion: It could be concluded that administration of the combination of extracted meniran and pegagan was not toxic to haematology of the rats in all doses.

Keywords

meniran (Phyllanthus niruri) pegagan (Centella asiatica) subchronic test haematology

Article Details

License

Authors who publish in the Jurnal Kedokteran dan Kesehatan Indonesia agree to the following terms:

  1. Authors retain copyright and grant Jurnal Kedokteran dan Kesehatan Indonesia right of first publication with the work simultaneously licensed under a Creative Commons Attribution Licence that allows others to adapt (remix, transform, and build) upon the work non-commercially with an acknowledgement of the work's authorship and initial publication in Jurnal Kedokteran dan Kesehatan Indonesia.
  2. Authors are permitted to share (copy and redistribute) the journal's published version of the work non-commercially (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in Jurnal Kedokteran dan Kesehatan Indonesia.
How to Cite
Fatimah, S. F., Darmawan, E., Narwanti, I., Dzulhaifa, D., Wulandari, I. A., & Salma, R. P. (2019). Subchronic toxicity test on combination of extracted Phyllanthus niruri and Centella asiatica on haematology in rats. JKKI : Jurnal Kedokteran Dan Kesehatan Indonesia, 10(3), 255–264. https://doi.org/10.20885/JKKI.Vol10.Iss3.art8
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Alegantina S, Setyorini HA, Triwahyuni. Pengujian mutu dan penetapan kadar filantin dada ekstrak etanol herba meniran (Phyllanthus niruri L.). Buletin Penelitian Kesehatan. 2015; 43 (1): 11-16.
  2. Rivai H, Septika R, Boestari A. Karakteristik ekstrak herba meniran (Phyllantus niruri Linn) dengan analisa fluoresensi. Jurnal Farmasi Higea. 2013; 5(2): 15-21.
  3. Chong NJ dan Aziz Z. A systematic review on the chemical constituents of Centella asiatica. Research Journal of Pharmaceutical, Biological, and Chemical Science. 2011; 2(3): 445-59.
  4. Aldi Y, Artika D, Aria M, Pengaruh pemberian ekstrak etanol meniran (Phyllantus niruri L.) terhadap jumlah eritrosit, retikulosit, kadar hemoglobin dan nilai hematokrit pada mencit putih jantan. Prosiding Seminar Nasional dan Workshop Perkembangan Terkini Sains Farmasi dan Klinik IV. Padang. 2014.
  5. Ningsih S dan Wibowo. Efek imunostimulan ekstrak meniran (Phyllanthus niruri L.) secara in vivo pada tikus. Jurnal Bahan Alam Indonesia. 2011; 7(1): 15-8.
  6. Adedapo AA, Adegdayibi AY, Emikpe BO. Some clinico-phatological changes asociated with the aqueous extract of the leaves of Phyllanthus Amarus in rats. Phytotherapy research. 2005; 19: 971-6
  7. Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia. Pedoman uji toksisitas nonklinik secara in vivo. BPOM RI. 2014.
  8. Sulastry F. Uji toksisitas akut yang diukur dengan penentuan LD50 ekstrak daun pegagan (Centella asiatica (L.) urban) terhadap mencit Balb/c (Skripsi). Semarang: Universitas Dipenogoro. 2009.
  9. Kusumawardani KC. Uji toksisitas subkronis produk kombinasi eksrtrak rimpang kunyit dan herba meniran pada tikus betina galur wistar kajian terhadap parameter fungsi ginjal (Skripsi). Yogyakarta. Universitas Gadjah Mada. 2016.
  10. Hidayati N. Aktifitas kombinasi seledri, meniran, pegagan dan kunyit terhadap kadar eritrosit, hemoglobin, hematokrit dan trombosit pada tikus yang diinjeksi gentamisin (Skripsi). Yogyakarta. Fakultas Farmasi Universitas Ahmad Dahlan. 2018.
  11. Salma RP. Efek kombinasi serbuk ekstrak meniran (Phyllanthus niruri L.) dan pegagan (Centella asiatica L.) terhadap SGOT dan SGPT pada tikus galur wistar (Skripsi). Yogyakarta. Fakultas Farmasi Universitas Ahmad Dahlan. 2019.
  12. Wulandari IA. Uji toksisitas subkronik kombinasi ekstrak terstandarisasi meniran dan pegagan terhadap parameter fungsi ginjal pada tikus galur wistar (skripsi). Yogyakarta. Fakultas Farmasi Universitas Ahmad Dahlan. 2019.
  13. Ihedioha JI, Ugwuja JI, Noeluneke OA, Udeani IJ, Danieligwe G. Reference values for the haematology profile of conventional grade outbred albino mice (Mus musculus) in Nsukka. Eastern Nigeria: Animal Research International. 2012; 9(2):1601-12.
  14. Provan D. ABC of Clinical Haematology 2nd. London. BMJ. 2003.
  15. Gad SC. Animal models in toxicology 3rd Ed. Press Boca raton. CRC. 2016.
  16. Fitria L dan Sarto M. Profil Hematologi Tikus (Rattus novergicus Berkenhout, 1769) Galur Wistar Jantan dan Betina Umur 4,6 dan 8 Minggu. Jurnal Ilmiah Biologi Biogenesis. 2014; 2(2): 94-100.