Article Sidebar

Submitted
August 4, 2019
Accepted
November 24, 2020
Published
December 31, 2020
Download
PDF Untitled (Bahasa Indonesia) Untitled (Bahasa Indonesia)
Statistic
Read Counter : 652 Download : 657

Main Article Content

Abstract

Background: A stress, sleep deprivation, can cause imbalance of reactive oxygen species (ROS) that can damage DNA, lipids and proteins of spermatozoa, consequently leading to infertility.
Objective: The aim of this study is to investigate differences of morphology and DNA fragmentation with some models of sleep deprivation in spermatozoa of white male Wistar rat strain (Rattus novergicus).
Methods: This study was an experimental study with post-test-only with control group design. There were 30 male Wistar rats which were randomly divided into 5 groups: control group, paradoxical sleep deprivation (PSD) group, total sleep deprivation (TSD) group, sleep recovery (SR) group, PSD group + SR group, and TSD group + SR group. Stress induction was conducted for 5 days. Morphological abnormality of spermatozoa was observed by creating sperm removal tinged by eosin and nigrosine. DNA fragmentation was performed by determining DNA fragmentation index (DFI). Morphological data of spermatozoa were analysed by One-Way Annova and Post-Hoc Turkey test, and DNA fragmentation of spermatozoa was analysed by Kruskal Wallis and Mann Whitney test to determine differences among the groups.
Results: The highest mean of the abnormal morphology was in PSD Group (89.5 %, p=0.004). The normal mean of DNA fragmentation was found in control group, PSD+SR group and TSD+SR group, PSD and TSD groups. Bad DNA fragmentation index was found in PSD and TSD groups (30.16% and 31.4%, p=0.023).
Conclusion: There were differences of morphology and DNA fragmentation of the spermatozoa in the male Wistar rat strain induced by various models of sleep deprivation.

Article Details

License

Authors who publish in the Jurnal Kedokteran dan Kesehatan Indonesia agree to the following terms:

  1. Authors retain copyright and grant Jurnal Kedokteran dan Kesehatan Indonesia right of first publication with the work simultaneously licensed under a Creative Commons Attribution Licence that allows others to adapt (remix, transform, and build) upon the work non-commercially with an acknowledgement of the work's authorship and initial publication in Jurnal Kedokteran dan Kesehatan Indonesia.
  2. Authors are permitted to share (copy and redistribute) the journal's published version of the work non-commercially (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in Jurnal Kedokteran dan Kesehatan Indonesia.
How to Cite
Agatri, N., Arjadi, F., & Rujito, L. (2020). Morphology and DNA fragmentation spermatozoa in animal models with sleep deprivation-induced stress. JKKI : Jurnal Kedokteran Dan Kesehatan Indonesia, 11(3), 232–240. https://doi.org/10.20885/JKKI.Vol11.Iss3.art4
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Orzel-Gryglewska J. Consequences of sleep deprivation. International Journal of Occupation Medical Environment Health. 2010;23(1):95–114.
  2. Eacker SM, Agrawal N, Qian K, Dichek HL, Gong E-Y, Lee K, et al. Hormonal regulation of testicular steroid and cholesterol homeostasis. Molecular Endocrinology 2008;22(3):623–35
  3. Radstaak M, Geurts SAE, Beckers DGJ, Brosschot JF, Kompier MAJ. Work Stressors, Perseverative Cognition and Objective Sleep Quality: A Longitudinal Study among Dutch Helicopter Emergency Medical Service (HEMS) Pilots. Journal of Occupational Health. 2014;56(6):469–77.
  4. Purwaningsing W. Fragmentasi dna spermatozoa pada tikus jantan (rattus norvegicus) diabetes mellitus yang diinduksi streptozotocin dapat diturunkan dengan pemberian suspensi bubuk kedelai kuning. In: Prosiding Seminar Ilmiah Nasional Kesehatan. 2013. p. 171–8.
  5. Lestari SW, Sari T. Fragmentasi DNA Spermatozoa: Penyebab, Deteksi, dan Implikasinya pada Infertilitas Laki-Laki. eJournal Kedokteran Indonesia. 2015;3(2):152–60.
  6. Alvarenga TA, Hirotsu C, Mazaro-Costa R, Tufik S, Andersen ML. Impairment of male reproductive function after sleep deprivation. Fertility Sterility. 2015;103(5):1355-1362.
  7. Vidyawati V, Moeloek N. Effectivity of Testosterone Contraception that caused Azoospermia and Oligospermia in Fertile Men. Majalah Kedokteran Indonesia. 2000;50(8):386–8.
  8. Akashi T, Watanabe A, Komiya A, Fuse H.. Evaluation of the sperm motility analyzer system (SMAS) for the assessment of sperm quality in infertile men. Systems Biology in Reproductive Medicine. 2010;56(6):473-7
  9. Agarwal A, Bragais FM, Sabanegh E. Assessing Sperm Function. Urologic Clinics of North America. 2008;35(2):157–71.
  10. Arjadi F, Partadireja G, Setyawan LM, M P. Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats. Universa Medicina. 2015;34(2):87–95.
  11. Everson CA, Bergmann BM, Rechtschaffen A. Sleep deprivation in the rat: III. Total sleep deprivation. Sleep. 1989;12(1):13–21.
  12. Zager A, Andersen ML, Ruiz FS, Antunes IB, Tufik S. Effects of acute and chronic sleep loss on immune modulation of rats. American Journal of Physiology Integrative Comparative. 2007;293(1):R504–9.
  13. Oh MM, Kim JW, Jin MH, Kim JJ, Moon DG. Influence of paradoxical sleep deprivation and sleep recovery on testosterone level in rats of different ages. Asian Journal of Andrology.. 2012;14(2):330–4.
  14. Dwijayanti F, Sukmaningsih A, Suarni N, Sudirga S, Parwanayoni N. Pemberian ekstrak buah juwet (syzygium cumini l.) Terhadap jumlah dan morfologi spermatozoa tikus putih (rattus sp.) Jantan yang terpapar asap rokok. Jurnal Simbiosis V. 2017;5(1):20–4.
  15. Riyadhi M, Arifiantini R, Purwantara B. Kajian morfologi spermatozoa sapi simmetal di beberapa Balai Inseminasi buatan Indonesia. Jurnal Hemera Zoa. 2010;1(2):1–6.
  16. Wahyuni L, Adnil E, Eliza A. Pengaruh Gangguan Tidur Terhadap Kadar Hormon Testosteron dan Jumlah Spermatozoa pada Tikus Jantan Wistar. Jurnal Kesehatan Andalas. 2015;4(3):834–40.
  17. Venâncio DP, Andersen ML, Vilamaior PSL, Santos FC, Zager A, Tufik S, et al. Sleep deprivation alters rat ventral prostate morphology, leading to glandular atrophy: a microscopic study contrasted with the hormonal assays. Journal of Biomedicine and Biotechnology. 2012:285938.
  18. Nurdin A. Psikoneuroimunologi Dasar. 5th ed. Jakarta: Penerbit EGC; 2013.
  19. Hipólide DC, Suchecki D, Pimentel de Carvalho Pinto A, Chiconelli Faria E, Tufik S, Luz J. Paradoxical sleep deprivation and sleep recovery: effects on the hypothalamic-pituitary-adrenal axis activity, energy balance and body composition of rats. Journal Neuroendocrinology. 2006;18(4):231–8.
  20. Lewis SEM, Aitken RJ. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell and Tissue Reseach. 2005;322(1):33–41.
  21. Villafuerte G, Miguel-Puga A, Rodríguez EM, Machado S, Manjarrez E, Arias-Carrión O. Sleep deprivation and oxidative stress in animal models: A systematic review. Oxidative Medicine and Cellular Longevity . 2015;234952
  22. Cheung V, Yuen VM, Wong GTC, Choi SW. The effect of sleep deprivation and disruption on DNA damage and health of doctors. Anaesthesia. 2019;74(4):434–40.
  23. Jay SM, Lamond N, Ferguson SA, Dorrian J, Jones CB, Dawson D. The Characteristics Of Recovery Sleep When Recovery Opportunity Is Restricted. Sleep. 2007;30(3):353–60.