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Submitted
November 4, 2019
Accepted
December 30, 2020
Published
December 31, 2020
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Abstract

Background: Methicillin-Resistant Staphylococcus aureus (MRSA) has become a major pathogene and its incidence continues to increase in various countries. The resistance to methicillin is caused by the mecA gene that encodes the expression of Penicillin Binding Protein 2a (PBP2a) so it has a low affinity for beta-lactam antibiotics. One of the factors that can increase the incidence of MRSA is inappropriate antibiotics use.
Objective: This study is to identify presence of the mecA gene in MRSA and to compare histories of inappropriate antibiotic uses of healthcare workers and communities in Banyumas.
Methods: This was a cross-sectional study conducting 120 samples from nasal swabs. Isolates of MRSA tested by bacteriological examinations and PCR of the mecA genes. Histories of antibiotic uses were assessed by using questionnaires and then analysed descriptively by using Fisher Exact test (SPPS, version 20).
Result: One sample of S. aureus from the microbiology examination (0.83%) was an MRSA although there was no a mecA gene identified by using the PCR method. 66.1% of the healthcare workers and 88,3% of the communities used antibiotics inappropriately. The results showed that there was a statistically significant difference in the histories of irrational uses of antibiotics (p?0.05).
Conclusion: A number of the communities who used inappropriate antibiotics was higher than the health workers. Furthermore, one MRSA strain with a negative mecA gene was found in the health worker group.

Keywords

antibiotics MRSA healthcare workers community

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How to Cite
Susanti, M. A., Mahardhika, G. S., Rujito, L., Darmawan, A. B., & Anjarwati, D. U. (2020). The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas. JKKI : Jurnal Kedokteran Dan Kesehatan Indonesia, 11(3), 257–265. https://doi.org/10.20885/JKKI.Vol11.Iss3.art7
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References

  1. Otsuka T, Yoshida K, Komiyama K, Ishikawa Y, Zaraket H, Okazaki M. Prevalence of methicillin-resistant Staphylococcus aureus among children in a region with controlled antimicrobial use. Japan Journal Infection Disease. 2011; 64(5):436-8.
  2. Deurenberg RH, Stobberingh EE. The evolution of Staphylococcus aureus. Infection, Genetics and Evolution. 2008; 8(6): 747-763.
  3. Gordon RJ, Lowy FD. Pathogenesis of Methicillin-Resistant Staphylococcus aureus Infection. Clinical Infection Disease. 2008; 46(2) : S350–S359.
  4. Flanders SA. Collard HR, Saint S. Nosocomial pneumonia: state of the science. American Journal Infection Control. 2016;34:84–93.
  5. Girard. Prevention of Hospital-acquired Infections. Departement Community Disease Surveillance Response. 2017;2:72.
  6. Chen CJ, Huang YC. New epidemiology of Staphylococcus aureus infection in Asia. (Themed Section: New epidemiology of Staphylococcus aureus infections.). Clinical Microbiology Infection. 2014; 20(7):605-23
  7. Anjarwati DU, Rujito L. Perawat Kesehatan sebagai Karier Methicilin Resistant Staphylococcus aureus ( MRSA ) Di Rumah Sakit Pemerintah dan Rumah Sakit Swasta Kabupaten Banyumas. Mandala Health. 2011; 5(1) : 241-247
  8. Hadi U, Duerink DO, Lestari ES, Nagelkerke NJ, Werter S, Keuter M, et al. Survey of antibiotic use of individuals visiting public healthcare facilities in Indonesia. International Journal Infection Disease. 2008; 12(6):622-9
  9. Pulungan S. Hubungan Tingkat Pengetahuan tentang Antibiotika dan Penggunaannya Dikalangan Mahasiswa Non Medis (Thesis). Medan: Universitas Sumatera Utara, 2011.
  10. Kementerian Kesehatan Republik Indonesia. PERMENKES RI NO 2406/MENKES/PER/XII/2011 Pedoman Umum Penggunaan Antibiotik. Permenkes RI. 2011; Jakarta, Republik Indonesia.
  11. Santosaningsih D, Santoso S, Budayanti NS, Kuntaman K, Lestari ES, Farida H, et al. Epidemiology of Staphylococcus aureus harboring the mecA or Panton-Valentine leukocidin genes in hospitals in Java and Bali, Indonesia. American Journal Tropical Medicine Hygiene. 2014; 90(4):728–34.
  12. Anggraini AD, Koendhori EB, Pramono H, Wahyono DD. Polymorphism Analysis of the Coagulase Gene in Isolates of Methicillin-Resistant Staphylococcus aureus with AluI Restriction Sites. Health Science Journal Indonesesia. 2017; 8(1):1–6.
  13. Clinical Laboratory Standard Institute, Performance Standards for Antimicrobial Susceptibility Testing, 26th edition, Wayne, US: 2018.
  14. Mahardhika GS, Susanti MA , Rujito L, Anjarwati DU. Detection Of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained From HealthCare Workers And Healthy Communities In Banyumas, Indonesia. Journal of Biomedicine and Translational Research. 2020; 6(1): 15-18.
  15. Kemalaputri DW, Jannah SN, Budiharjo A. Deteksi MRSA (Methicillin Resistant Staphylococcus aureus) Pada Pasien Rumah Sakit Dengan Metode Maldi-Tof MS dan Multiplex PCR, Jurnal Biologi. 2017; 6(4):51-61
  16. Nismawati. Deteksi Methicillin Resistant Staphylococcus aureus (MRSA) Pada Pasien Rumah Sakit Universitas Hasanuddin Dengan Metode Kultur. Prosiding Seminar Biologi, Fakultas Science dan Technology, Universitas Islam Negeri Alauddin Makassar. 2018; 4(7).
  17. Cikman A, Aydin M, Gulhan B, Karakecili F, Kurtoglu MG, Yuksekkaya S, et al. Absence of the mecC gene in methicillin-resistant Staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkey. Journal Infection Public Health [Internet]. 2019; 12(4):528–33.
  18. Green BN, Johnson CD, Egan JT, Rosenthal M, Griffith EA, Evans MW. Methicillin-resistant Staphylococcus aureus: an overview for manual therapists. Journal of Chiropractic Medicine.2013; 11(1): 64–76.
  19. Putra MIH, Suwarto S, Loho T, Abdullah M. Faktor Risiko Methicillin Resistant Staphylococcus aureus pada Pasien Infeksi Kulit dan Jaringan Lunak di Ruang Rawat Inap. Jurnal Penyakit Dalam Indonesia. 2017;1(1):3.
  20. Creech CB, Saye E, McKenna BD, Johnson BG, Jimenez N, Talbot TR. One-year surveillance of methicillin-resistant Staphylococcus aureus nasal colonization and skin and soft tissue infections in collegiate athletes. Arch Pediatr Adolesc Med. 2010;164(7):615–620.
  21. Cesur S, Yildiz E, Irmak H, Aygün Z, Karakoç E, Kinikli S DA. Evaluation of oxacillin resistance screening agar and chromogenic MRSA agar media for the detection of methicillin resistance in Staphylococcus aureus clinical isolates. Mokrobiyology Buletine. 2014; 44(2):279–84.
  22. Goyal A. Prevalence and Antimicrobial Susceptibility Pattern of Methicillin-resistant Staphylococcus aureus [MRSA] isolates at a Tertiary Care Hospital in Agra, North India – A systemic annual review. Journal Dental Medicine Science. 2017;11(6):80–4.
  23. Elhassan MM, Ozbak HA, Hemeg HA, Elmekki MA, Ahmed LM. Absence of the mecA gene in methicillin resistant Staphylococcus aureus isolated from different clinical specimens in Shendi City, Sudan. Biomedical Resistance International. 2015; 2015(895860).
  24. García-Garrote. Methicillin-resistant Staphylococcus aureus carrying the mecC gene: emergencein Spain and report of a fatal case of bacteraemia. Journal Antimicrobiology Chemotherapy. 2014;47:137–46.
  25. Ba X, Harrison EM, Edwards GF, Holden MTG, Larsen AR, Petersen A, et al. Novel mutations in penicillin-binding protein genes in clinical Staphylococcus aureus isolates that are methicillin resistant on susceptibility testing, but lack the mec gene. Journal Antimicrobiology Chemotherapy. 2014; 69(3):594-7
  26. Becker K, van Alen S, Idelevich EA, Schleimer N, Seggewiß J, Mellmann A, et al. Plasmid-encoded transferable mecb-mediated methicillin resistance in staphylococcus aureus. Emergency Infection Disease. 2018;24(2):242–8.
  27. Voidazan, S, Moldovan G, Voidazan L, Zazgyva A, Moldovan H. Knowledge, Attitudes And Practices Regarding The Use Of Antibiotics. Study On The General Population Of Mures County, Romania. Infection and Drug Resistance. 2019;12: 3385–3396.