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Submitted
April 5, 2021
Accepted
August 24, 2021
Published
August 31, 2021
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Abstract

Background: Genus Trichoderma of fungal kingdom are largely used as biological control agents due to broad-spectrum activity against plant pathogens.
Objective: This study aimed to investigate Trichoderma harzianum, an endophytic fungus obtained from ginger (Zingiber officinale) leaves.
Methods: The chemical structures of the isolated compounds were deduced on the basis of UV, 1H NMR and MS data analyses, as well as comparison with literature.
Results: Two known tetramic acid derivatives were isolated from this fungus, including harzianic acid (A) and isoharzianic acid (B). Compound B inhibited the growth of a corn pathogenic fungus, Ustilago maydis, with inhibition zone diameter (39 ± 0.33 mm) larger than nystatin (29 mm). Additionally, iso-HA (B) revealed antibacterial effect towards Staphylococcus aureus with MIC value of 25 µM. However, both compounds showed no cytotoxicity against human cervical and ovarian cancer cell lines.
Conclusion: T. harzianum produced antimicrobial compound like iso-HA which has potential application either in agricultural or health.

Keywords

Trichoderma harzianum endophytic fungus antimicrobial isoharzianic acid

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How to Cite
Harwoko, H., Lee, J., Daletos, G., Feldbrügge, M., Kalscheuer, R., & Proksch, P. (2021). Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale). JKKI : Jurnal Kedokteran Dan Kesehatan Indonesia, 12(2), 151–157. https://doi.org/10.20885/JKKI.Vol12.Iss2.art8
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