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Submitted
April 5, 2021
Accepted
August 24, 2021
Published
August 31, 2021
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Abstract

Background: Genus Trichoderma of fungal kingdom are largely used as biological control agents due to broad-spectrum activity against plant pathogens.
Objective: This study aimed to investigate Trichoderma harzianum, an endophytic fungus obtained from ginger (Zingiber officinale) leaves.
Methods: The chemical structures of the isolated compounds were deduced on the basis of UV, 1H NMR and MS data analyses, as well as comparison with literature.
Results: Two known tetramic acid derivatives were isolated from this fungus, including harzianic acid (A) and isoharzianic acid (B). Compound B inhibited the growth of a corn pathogenic fungus, Ustilago maydis, with inhibition zone diameter (39 ± 0.33 mm) larger than nystatin (29 mm). Additionally, iso-HA (B) revealed antibacterial effect towards Staphylococcus aureus with MIC value of 25 µM. However, both compounds showed no cytotoxicity against human cervical and ovarian cancer cell lines.
Conclusion: T. harzianum produced antimicrobial compound like iso-HA which has potential application either in agricultural or health.

Keywords

Trichoderma harzianum endophytic fungus antimicrobial isoharzianic acid

Article Details

How to Cite
Harwoko, H., Lee, J., Daletos, G., Feldbrügge, M., Kalscheuer, R., & Proksch, P. (2021). Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale). JKKI : Jurnal Kedokteran Dan Kesehatan Indonesia, 12(2), 151–157. https://doi.org/10.20885/JKKI.Vol12.Iss2.art8
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