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Submitted
March 9, 2020
Accepted
August 31, 2020
Published
August 31, 2020
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Abstract

Background: Obesity can lead to insulin resistance contributing to diabetes mellitus (DM). Empirically, insulin leaves of Tithonia diversifolia (Hems.) A. Gray (Td) can be used as a traditional DM medicine.
Objective: This study aims to determine effects of Td methanol extracts on insulin resistance and secretion in DM rats induced by a high-fat diet and alloxan.
Methods: This study was a randomized pre and post-test control group. Its research subjects were divided into five groups: a normal group, an obese group with diabetes without therapy (KN), an obese group with DM treated by the Td extracts with a dose 50 mg/kg, 250 mg/kg, and 500 mg/kg. The DM obesity was induced by a high-fat diet followed by administration of alloxan with a dose of 135 mg/kg intraperitoneally. The Td extracts was administered after 7 days of DM induction by using gastric swabs. Insulin resistance in the rats was measured based on HOMA-IR index values, while their insulin secretion was based on HOMA-B values. A statistical analysis was conducted by a 95% confidence level.
Results: Blood glucose levels decreased to normal levels in all the groups administered by the Td extracts, except in the KN group. The HOMA-IR index values in all groups treated by the Td extracts did not increase except in the KN group. The HOMA-B index values in groups with doses of 50 and 250 mg/kgBW experienced a higher increase and significantly differed with the KN group.
Conclusion: The administration of the Td extracts with doses of 50 and 250 mg/ kgBW in the DM rats could decrease blood glucose levels, could prevent insulin resistance and could increase insulin secretion.

 

Keywords

Obesity diabetes mellitus Tithonia diversifolia (Hemsl.) A. Gray Wistar rats HOMA-IR index HOMA-B index insulin resistance insulin secretion

Article Details

How to Cite
Fitriyanto, R. E., Sugiarto, S., & Ardiyanto, D. T. (2020). Effects of methanol extracts of insulin leaves (Tithonia diversifolia (hemsl.) A. Gray) on insulin resistance and secretion of alloxan induced-obese diabetic rats. JKKI : Jurnal Kedokteran Dan Kesehatan Indonesia, 11(2), 180–190. https://doi.org/10.20885/JKKI.Vol11.Iss2.art11
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References

  1. Skyler JS, Bakris GL, Bonifacio E, Darsow T, Eckel RH, Groop L, et al. Differentiation of diabetes by pathophysiology, natural history, and prognosis. Diabetes. 2017;66:241–55.
  2. World Health Organization. Appropriate body-mass index for Asian populations and its implications for policy and intervention strategies. The Lancet. 2004;363.
  3. Organization WH. Global health observatory (GHO) data. 2015.
  4. Abel ED, O’Shea KM, Ramasamy R. Insulin resistance: Metabolic mechanisms and consequences in the heart. Arteriosclerosis, Thrombosis, and Vascular Biology. 2012;32(9):2068–2076.
  5. Malik VS, Willett WC, Hu FB. Global obesity: trends, risk factors and policy implications. Nature Reviews Endocrinology. 2012;9:13–27.
  6. Gadde KM, Martin CK, Berthoud H-R, Heymsfield SB. Obesity, pathophysiology and management. Journal of The American College of Cardiology [Internet]. 2018;71. Available from: https://doi.org/10.1016/j.jacc.2017.11.011
  7. Czech MP. Insulin action and resistance in obesity and type 2 diabetes. Nature Medicine. 2017;23:804–14.
  8. Hectors TLM, Vanparys C, Ven K van der, Martens GA, Jorens PG, Gaal LF Van, et al. Environmental pollutants and type 2 diabetes: a review of mechanisms that can disrupt beta cell function. Diabetologia. 2011;54:1273–129.
  9. International Diabetes Federation (IDF). Diabetes Atlas. 2014.
  10. BPP Kementerian Kesehatan. Riset kesehatan dasar tahun. 2013.
  11. Kementerian Kesehatan BP dan P. Riset kesehatan dasar tahun 2018. 2018.
  12. Preethi PJ. Herbal medicine for diabetes mellitus: A review. International Journal of Phytopharmacy. 2013;3.
  13. Mohamed Eddouks, Amina Bidi, Bachir El Bouhali, Lhoussain Hajji NAZ. Antidiabetic plants improving insulin sensitivity. .Journal of Pharmacy and Pharmacology. 2014;66:1297-1214.
  14. Song I, Patel O, Himpe E, Muller CJF, Bouwens L. Beta cell mass restoration in alloxan-diabetic mice treated with EGF and gastrin. PLoS ONE. 2015;10(10).
  15. N.I. El-Desouki, G.A. Tabl, K.K. Abdel-Aziz EISN. Improvement in beta-islets of Langerhansin alloxan-induced diabetic rats by erythropoietinand spirulina. The Journal of Basic & Applied Zoology. 2015;71:20–31.
  16. FuenteValerón P, Pablos-Velasco PL d. Limitations of insulin-dependent drugs in the treatment of type 2 diabetes mellitus. Medicina Clínica. 2013;141:20–5.
  17. Hiransai P, Tangpong J, Kumbuar C, Hoonheang N, Rodpech O, Sangsuk P, et al. Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia. Asian Pacific Journal of Tropical Biomedicine. 2016;6(11):950–6.
  18. Suryo A, Wahyuningsih S, Syarif R, Suharmi S, Murini T, Saputra F. Selektivitas ekstrak terpurifikasi daun Tithonia diversifolia (Hemsley) A.Gray) Terhadap sel hela. Tradistional Medicine Journal. 2013;18:22–8.
  19. Miura T, Nosaka K, Ishii H, Ishida T. The herb Tithonia diversifolia, in KK-Ay diabetic mice. Biological and Pharmaceutical Bulletin Journal. 2003;28:2152–4.
  20. Lin H. Sesquiterpene lactones from Tithonia diversifolia act as peroxisome proliferator-activated receptor agonists. Bioorganic & Medical Chemistry Letters. 2012;22:2954–8.
  21. Murti B. Desain dan ukuran sampel untuk penelitian kuantitatif dan kualitatif di bidang kesehatan. Yogyakarta: Gadjah Mada University Press; 2013.
  22. Supriyadi. Statistik kesehatan. Jakarta: Salemba Medika; 2014.
  23. Ballesteros LF, Teixeira JA, Mussatto SI. Selection of the solvent and extraction conditions for maximum recovery of antioxidant phenolic compounds from coffee silverskin. Food and Bioprocess Technology. 2014;7:1322–1332.
  24. Gama, Da RM, Guimarães, Abreu LC de, Marcelo, Armando-Juniora, et al. Phytochemical screening and antioxidant activity of ethanol extract of Tithonia diversifolia (Hemsl) A. Gray dry flowers. Asian Pacific Journal of Tropical Biomedicine. 2014;4(9):740–2.
  25. Gajda A. High fat diets for diet-induced obesity models: A brief review of the scientific literature. www. ResearchDiets.com. 2008.
  26. Hariri N, Thibault L. High-fat diet-induced obesity in animal models. Nutrition Research Reviews. 2010;23:270–299.
  27. Novelli E, Diniz Y, Galhardi C, Ebaid G, Rodrigues H, Mani F, et al. Anthropometrical parameters and markers of obesity in rats. Laboratory Animals Journal. 2007;41:111–119.
  28. Kusumawati D. Bersahabat dengan Hewan Coba. Yogyakarta: Gabjah Mada University Press; 2004.
  29. Ighodaro OM, Adeosun AM, Akinloye OA. Alloxan-induced diabetes, a common model for evaluatingthe glycemic-control potential of therapeutic compoundsand plants extracts in experimental studies. Medicina. 2017;53:365–74.
  30. Nugroho A. Hewan Percobaan Diabetes Melitus: Patologi dan Mekanisme Aksi Diabetogenik. Biodiversitas. 2006;7:378–82.
  31. Ki-Chul Sung, Gerald M. Reaven SHK. Utility of homeostasis model assessment of beta-cell function in predicting diabetes in 12,925 healthy Korean. Diabetic Care Journal. 2013;33:2000–2.
  32. Yun Li, Lihua Zhang, Yong Gu, Chuanming Hao TZ. Insulin resistance as a predictor of cardiovascular disease in patients on peritoneal dialysis. Sage [Internet]. 2013;33(4). Available from: https://doi.org/10.3747%2Fpdi.2012.00037
  33. Petersen MC, Shulman GI. Mechanisms of Insulin Action and Insulin Resistance. Physiological Reviews [Internet]. 2018;98:2133–2223. Available from: https://doi.org/10.1152/physrev.00063.2017
  34. Musi N, Guardado-Mendoza R. Adipose Tissue as an Endocrine Organ. In: Cellular Endocrinology in Health and Disease. 2014. p. 229–37.
  35. Shoelson SE, Herrero L NA. Obesity, inflammation, and insulin resistance. Gastroenterology. 2007;132:2169–2180.
  36. Ye J. Mechanisms of insulin resistance in obesity. Frontiers of Medicine [Internet]. 2013;7(1):14–24. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936017/pdf/nihms556133.pdf
  37. Thongsom M, Chunglok W, Kuanchuea R, Tangpong J. Antioxidant and hypoglycemic effect of Tithonia diversifolia aqueous leaves extract in alloxan-induced diabetic mice. Advance in Environmental Biology. 2013;7:2116–25.
  38. Claudia Di Giacomo, Luca Vanella, Valeria Sorrenti, Rosa Santangelo, Ignazio Barbagallo, Giovanna Calabrese, Carlo Genovese, Silvana Mastrojeni, Salvatore Ragusa RA. Effects of Tithonia diversifolia (Hemsl.) A. Gray extract on edipocyte differentiation of human mesenchymal stem cells. Plos One. 2015;
  39. Olukunle JO, Okediran BS, Sogebi EA, Jacobs EB. Hypoglycaemic and hypolipidaemic effects of the aqueous leaf ekstract of tithonia diversifolia. Annual Research and Review in Biology. 2016;4:2656–62.
  40. Savage DB, Petersen KF, Shulman GI. Mechanisms of insulin resistance in humans and possible links with inflammation. Vol. 45, Hypertension. 2005. p. 828–33.
  41. Samuel VT, Shulman. GI. Integrating mechanisms for insulin resistance: Common threads and missing links. Cell. 2012;148:852–871.
  42. Hotamisligil G. Inflammatory pathways and insulin action. International Journal of Obesity Relation to Metabolic Disorder. 2003;27:S53–S55.
  43. Samuel Treviñoa, Michael P.Waalkes, José Angel Flores Hernández, Bertha Alicia León-Chavez, Patricia Aguilar-Alonso EB. Chronic cadmium exposure in rats produces pancreatic impairment and insulin resistance in multiple peripheral tissues. https://www.sciencedirect.com/science/journal/00039861 [Internet]. 2015;27–35. Available from: Author links open overlay panelSamuelTreviñoaMichael P.WaalkesbJosé AngelFlores HernándezcBertha AliciaLeón-ChavezaPatriciaAguilar-AlonsoaEduardoBrambilaa
  44. Owoyele VB, Wuraola CO, Soladoye AO, Olaleye SB. Studies on the antiinflammatory and analgesic properties of tithonia diversifolia leaf exstract. Journal of Ethnopharmacology. 2004;90:314–21.
  45. Chi-Long Juang, Fei Shish Yang, Ming Shuang Hsieh, Hu-Yi Tseng, Su-Chiu Chen H-CW. Investigation of anti-oxidative stress in vitro and water apparent diffusion coefficient in MRI on rat after spinal cord injury in vivo with Tithonia diversifolia ethanolic extracts (TDE) Treatment. BMC Complement Alternative Medicine. 2014;14:447.