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Submitted
March 9, 2020
Accepted
August 31, 2020
Published
August 31, 2020
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Abstract

Background: Obesity can lead to insulin resistance contributing to diabetes mellitus (DM). Empirically, insulin leaves of Tithonia diversifolia (Hems.) A. Gray (Td) can be used as a traditional DM medicine.
Objective: This study aims to determine effects of Td methanol extracts on insulin resistance and secretion in DM rats induced by a high-fat diet and alloxan.
Methods: This study was a randomized pre and post-test control group. Its research subjects were divided into five groups: a normal group, an obese group with diabetes without therapy (KN), an obese group with DM treated by the Td extracts with a dose 50 mg/kg, 250 mg/kg, and 500 mg/kg. The DM obesity was induced by a high-fat diet followed by administration of alloxan with a dose of 135 mg/kg intraperitoneally. The Td extracts was administered after 7 days of DM induction by using gastric swabs. Insulin resistance in the rats was measured based on HOMA-IR index values, while their insulin secretion was based on HOMA-B values. A statistical analysis was conducted by a 95% confidence level.
Results: Blood glucose levels decreased to normal levels in all the groups administered by the Td extracts, except in the KN group. The HOMA-IR index values in all groups treated by the Td extracts did not increase except in the KN group. The HOMA-B index values in groups with doses of 50 and 250 mg/kgBW experienced a higher increase and significantly differed with the KN group.
Conclusion: The administration of the Td extracts with doses of 50 and 250 mg/ kgBW in the DM rats could decrease blood glucose levels, could prevent insulin resistance and could increase insulin secretion.

 

Keywords

Obesity diabetes mellitus Tithonia diversifolia (Hemsl.) A. Gray Wistar rats HOMA-IR index HOMA-B index insulin resistance insulin secretion

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How to Cite
Fitriyanto, R. E., Sugiarto, S., & Ardiyanto, D. T. (2020). Effects of methanol extracts of insulin leaves (Tithonia diversifolia (hemsl.) A. Gray) on insulin resistance and secretion of alloxan induced-obese diabetic rats. JKKI : Jurnal Kedokteran Dan Kesehatan Indonesia, 11(2), 180–190. https://doi.org/10.20885/JKKI.Vol11.Iss2.art11
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