The cytotoxic effect of pinostrobin fingerroot (boesenbergia pandurata)on the culture of hela cells
Objective : To assess cytotoxicity effect of pinostrobin temu kunci on HeLa cell culture
Methods : This study used simple experimental design. Pinostrobin were isolated from temu kunci and proved by TLC densitometry compared to standard pinostrobin. HeLa cell culture were treated with pinostrobin with concentrations 5, 25, 50, 75, 100, and 250 ug/mL. Cytotoxicity test were performed by MTT assay. Data were analyzed using one-way ANOVA.
Results : There was significant difference (p=0.000) of means of cell
viability percentage, respectively: 92.58 Â± 9.84 (5Î¼g/mL), 91.78 Â± 4.4 (25Î¼g/mL), 80.09 Â± 4.51 (50Î¼g/mL), 76.89 Â± 7.75 (75Î¼g/mL), 67.85 Â± 11.31 (100Î¼g/mL), dan 48.82 Â± 16.61 (250Î¼g/mL). The IC50 was 250Î¼g/mL.
Conclusion : Pinostrobin showed no active cytotoxicity effect on HeLa cell culture.