Pengaruh Suhu dan Interval Waktu Penyimpanan Sampel Serum pada Pengukuran Kadar Glukosa Darah
The number of samples should be checked often can not be done immediately examination due to theÂ limited number of laboratory personnel. This phenomenon is common in clinical laboratories and privateÂ land. This needs attention given the many factors that can affect test results. Pre-analytical factors thatÂ need to be considered is the examination time interval and sample storage temperature prior toÂ examination. Examination of blood glucose is one of the frequent checks at the hospital. The methodÂ often used is glucose oxidase method with the serum as a sample. The purpose of this research is to
determine how far the impact of delays in the inquest and the influence of storage temperature on serumÂ blood glucose levels. The study was conducted on 30 people consisting of patients and hospitalÂ employees who are willing. Criteria for inclusion were men or women aged 20 - 40 years old and willingÂ to follow the research. Exclusion criteria were suffering from diabetes, severe kidney disease, or areÂ pregnant. Each subject and blood samples taken as soon as possible done on a blood sampleÂ centrifugation to obtain serum. Serum from each sample was divided into 3 tubes, which will beÂ examined: immediate and delayed 4 hours and each tube is stored at a temperature of 2-8 Â°C and 25-28
Â°C. The parameters examined were the blood chemistry of blood glucose. Examination of blood glucoseÂ using glucose oxidase method. Sampling is performed by the method of consecutive (consecutiveÂ sample). Statistical analysis by independent samples t-test. The examination of serum samples from 30Â patients and hospital employees who meet the criteria of inclusion and exclusion. Comparison of resultsÂ of blood glucose levels at examination time delay between 0 hours and 4 hours to get results that are notÂ significant (p>0.05). And blood glucose levels between groups of temperature 25-28 Â°C and 2-8 Â°C noÂ significant difference in outcome (p>0.05). The interval 0 hour storage time and 4 hours at a temperatureÂ of 25- 28 Â°C (room temperature) and 2-8 Â°C (refrigerator temperature) does not affect the results of blood
glucose levels in serum samples.