Main Article Content
Abstract
Houttuynia cordata (H. cordata) is a medicinal plant that contains various secondary metabolites, especially flavonoids that have potential biological activity. This study aims to optimize the mobile phase and evaluate the chromatographic profile of ethyl acetate fractions of H. cordata leaves and rhizomes using TLC. Separation was performed on a silica gel stationary phase with several mobile phase variations, namely n-hexane and ethyl acetate (3:7; 5:10). The results showed that the n-hexane and ethyl acetate (3:7) mobile phase provided the most optimal separation. The leaf fraction was identified to contain quercetin, quercitrin, and kaempferol, while the rhizome fraction was identified to contain quercetin and kaempferol. In conclusion, the TLC method with n-hexane and ethyl acetate (3:7) as the mobile phase is effective for separating and profiling semipolar compounds in the ethyl acetate fraction of H. cordata and has the potential to support further research.
Keywords: Houttuynia cordata, Thin layer chromatography, TLC mobile phase, Phytochemical
Article Details
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).