Main Article Content
Abstract
Fucoidan is a Sulfated polysaccharide derived from brown seaweed, it has attracted considerable attention due to its various biological properties. Indonesia has riches diversity biological abundant sea. One of areas in the Special Region of Yogyakarta Province that have potential producing algae is Regency Gunung Kidul. The extracted fucoidan from brown algae using conventional method depends on dissolution wall cell polysaccharide in various conditions, such as hot water, acids /alkalis, and organic solvents. However, the approach this own shortcomings, namely time long extraction and results low yield. For overcome this problem, this study evaluates the effectiveness of combined ultrasound and enzyme extraction method in improving the yield and bioactive properties of fucoidan obtained from Sargassum Siliquosum. Cellulase enzyme concentrations (0.5%, 1%, and 2% v/v) were investigated and compared with the conventional extraction method. The result showed that the (UAE+EAE 2%) method provided the highest yield (3.622%), which is higher than the conventional method (3.506%), while the lower enzyme concentrations produced less yields. FTIR analysis ensured that the functional groups of fucoidan, including sulfate ester (S=O) and glycosidic (C-O-C) bonds. The effective extraction of fucoidan was confirmed by High Performance Liquid Chromatography (HPLC) analysis, and the DPPH assay's evaluation of antioxidant activity showed that the bioactive characteristics of fucoidan were maintained. Overall, in comparison to conventional extraction methods, the combined UAE+EAE approach shows great promise as a more effective and eco-friendlier fucoidan extraction technology. Further optimization and purification for the extracted fucoidan are recommended to improve bioactivity for future applications.
Article Details
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).