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January 12, 2016
Published
January 31, 2015
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Abstract

Tumor merupakan proses terjadinya pertumbuhan sel yang tidak beraturan dan tak terkontrol (abnormal). Tumor ini bisa bersifat kanker (malignant) atau non-kanker (jinak). Dengan dilakukan penelitan terhadap aktivitas sel lymphoma terhadap kombinasi ekstrak temu putih dan bawang putih menggunakan metode MTT Assay bertujuan untuk menguji keefektifan dari kombinasi ekstrak tersebut terhadap aktifitas sel lymphoma. Bawang putih (Allium sativum) memiliki senyawa organosulfur yang dapat menekanan proliferasi sel dengan menghalangi perkembangan siklus sel tanpa diikuti kerusakan sel disekitarnya. Sedangkan temu putih (Curcuma zedoaria) memiliki mekanisme anti kanker dengan menginduksi apoptosis dari sel kanker itu sendiri tanpa merusak sel lain disekitarnya. Kombinasi ekstrak tersebut diharapkan dapat member efek sinergi dalam penghambatan aktivitas tumor lymphoma. Serbuk simplisia dibuat dalam bentuk ekstrak dengan menggunakan metode maserasi dan menggunakan pelarut etanol 70%. Maserat yang telah diperoleh akan disaring dan diuapkan dengan penguap vakum atau penguap tekanan rendah hingga diperoleh ekstrak kental. Ekstrak kental kemudian diujikan pada sel lymphoma dengan seri dosis 50 : 50 ( 500 : 500 ; 250 : 250 ; 125 : 125 ; 62,5 : 62,5 ; 31,25 : 31,25 ; 15, 625 ; 15, 625 ppm) yang telah dibiakkan pada media komplit di dalam mikroplate dan diberi reagen MTT Assay 0,5%. Hal ini bertujuan untuk mengamati besarnya persen kematian sel (%) dan kristal formazan yang terbentuk. Data hasil pengamatan yang diperoleh, dianalisis dengan menggunakan Elisa reader pada panjang gelombang 550 nm.

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Author Biographies

Kharismatul Khasanah, Universitas Islam Indonesia

Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Diny Lidy, Universitas Islam Indonesia

Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam
How to Cite
Arifah, I. S., Khasanah, K., Lidy, D., & Pandapotan, H. (2015). KOMBINASI EKSTRAK TEMU PUTIH (CURCUMA ZEDOARIA) DAN BAWANG PUTIH (ALLIUM SATIVUM L.) TERHADAP AKTIVITAS SEL LIMFOMA DENGAN METODE MTT ASSAY. Khazanah: Jurnal Mahasiswa, 7(2). https://doi.org/10.20885/khazanah.vol7.iss2.art3
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