Antiangiogenic Effect Of The Chloroform Extract Of Tinospora crispa (L.) Miers Stem In The Chick Embryo Chorioallantoic Membrane (CAM) Induced By bFGF
Cancer is one of the most complex disease involving molecular process cause it is hard to be cured. There are many natural compounds which have been used empirically in the society in order to treat cancer. One of them is a kind of herbal medicine called â€˜Brotowaliâ€™ (Tinospora crispa (L.) Miers). The objective of this research wasÂ to know antiangiogenic effect of the chloroform extract ofÂ brotowali stem using CAM method induced by bFGF. In this research, the inhibition test is done by the CAM at 9 day chick embryo dividedÂ intoÂ seven groups of treatment. Group I isÂ as the paper disc controller, group II as the bFGF controller, group III asÂ bFGF +Â DMSO 0,8% solvent controller, group IV, V, VI and VII, as the group that conduct the angiogenesis inhibition test. The last four group were given 10 ng of bFGF each and the chloroform extract of brotowali stem with the doses of 15 Î¼g/ml, 60 Î¼g/ml, 240 Î¼g/ml and 960 Î¼g/ml. After having been incubated for 3 days (egg at 12 day), CAM were carefully observedÂ macroscopically and microscopically. The result showed thatÂ the chloroform extract of brotowali stem canÂ inhibit the angiogenesis in CAM induced by bFGF. It show that the angiogenesis inhibition for the dose of the chloroform extract ofÂ brotowali stem were successively riseÂ due to the dose increment, with angiogenic potencyÂ 31,87Â±9,01%,Â 43,12Â±8,01%, 53,44Â±2,70% andÂ 62,81Â±4,74%.
Keywords : cancer, angiogenesis, Tinospora Â crispa (L.) Miers, bFGF, CAM
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