Article Sidebar

Submitted
March 28, 2024
Accepted
August 6, 2024
Published
October 29, 2024
Download
PDF
Statistic
Read Counter : 60 Download : 11

Main Article Content

Abstract

The  production of amylase produced by lactic acid bacteria (LAB) has better stability. The purpose of this study was to utilize and determine the characteristics of LAB isolates from porang skin waste which have the potential as probiotics and the activity of amylase enzymes. Isolation was done by fermentation method for 48 h with the addition of starter Pediococcus acidilactici that had been cultured. The results showed that the number of colonies of N1 isolates was 49 ×108 CFU/mL with regular small round colonies, having a convex elevation, the edges of the colonies were round, yellowish white, and Gram positive bacteria because they produced a purple color on Gram staining. Acid resistance test at pH 2.0; 3.0; 4.0; 5.0 and 6.0, the isolates N1 was able to survive at each pH because it showed turbidity in the growth medium. The antimicrobial activity test showed a strong zone of inhibition on S. aureus and S. pyogenes of 10,33 and 11,17 mm and a moderate zone of inhibition on K. pneumoniae, S. dysentriae, and P. aeruginosa, respectively, which was 6,33; 7,7 and 7,5 mm. The positive N1 isolate contained alkaloids as bioactive compounds from the results of phytochemical screening and the results of the FTIR analysis showed that specific groups of alkaloids were N-H, C-N, and C=O at wave numbers 3423.30 cm-1; 1235.45 cm-1; and 1634.81 cm-1.

Keywords

Lactic acid, Amylase, Probiotic, Porang Tubers

Article Details

License

Copyright (c) 2024 Habibi Hidayat, Nakita Heniar Septyana, Riang Adeko, Dinda Rizki Febriani, Amri Setyawati1

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
Hidayat, H., Nakita Heniar Septyana, Riang Adeko, Dinda Rizki Febriani, & Amri Setyawati1. (2024). Chemical Compound Identification of Lactic Acid Bacteria (LAB) From Porang Tubers Waste (A. muelleri blume) as Probiotic. EKSAKTA: Journal of Sciences and Data Analysis, 5(2), 154–161. https://doi.org/10.20885/EKSAKTA.vol5.iss2.art5
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

References

  1. H. Hidayat, E. Fadilah, Nurmadina, M. M. Musawwa, Bioconversion of anthocyanin to anthocyanidin from fermented fruit and vegetable waste by Leuconostoc mesenteroides as probiotik food material, IOP Conference Series: Earth and Environmental Science, 463 012100 (2020)
  2. Faridah, H. Durrotul, S. K. Sari, Utilization of Microorganism on the Development of Halal Food Based on Biotechnology, Journal of Halal Product and Research 2(1):33–43 (2019)
  3. Ginting, Ledy, Wijanarka, E. Kusdiyantini, Isolasi Bakteri Endofit Tanaman Pepaya (Carica papaya L.) dan Uji Aktivitas Enzim Amilase, Berkala Bioteknologi 3(2):1–7 (2020)
  4. Drajat, I. Surya, G. Cahya, E. Darma, R. Aryani, Karakterisasi dan Optimasi Tepung Porang (Amorphophallus muelleri) Sebagai Basis Sediaan Edible Film. Prosiding Farmasi 474–82 (6) (2020)
  5. Nindya, A. Afifah, Pemanfaatan Limbah Kulit Umbi Porang (Amorphophallus oncophillus) Sebagai Bahan Baku Pembuat Bioetanol.” Sekolah Tinggi Ilmu Farmasi (2013)
  6. H. Hidayat, Identifikasi Morfologi Dan Uji Aktivitas Antimikroba Terhadap Bakteri Escherichia coli Dari Fermentasi Buah Markisa (Passiflora Sp.), Jurnal Eksakta 15(1–2):76–85 (2015)
  7. Rahmiati, M. Mumpuni, Eksplorasi Bakteri Asam Laktat Kandidat Probiotik Dan Potensinya Dalam Menghambat Bakteri Patogen, Elkawnie 3(2):141–50 (2017)
  8. Wulandari, Destik, D. Purwaningsih. Identifikasi Dan Karakterisasi Bakteriamilolitik Pada Umbi Colocasia Esculental. Secara Morfologi, Biokimia dan Molekuler, Jurnal Bioteknologi & Biosains Indonesia 6(2):247–48 (2019)
  9. Novelni, Ringga, Ifmaily, A. Hanafiah, Identifikasi dan Uji Resistensi Bakteri Dari Swab (Usap) Tenggorokan Penyebab Pneumonia Pada Pasien Yang Di Rawat Inap Bangsal Paru Rsup Dr. M. Djamil Padang, Jurnal Akademi Farmasi Prayoga 5(2):50–62 (2016)
  10. Kiti, A. Ammalia, I. Jamilah, H. Rusmarilin, Aktivitas Antimikroba Isolat Bakteri Asam Laktat Yang Diisolasi Dari Pangan Pliek Terhadap Bakteri Staphylococcus aureus, Escherichia coli Dan Candida Albicans Secara in Vitro, Journal of Healthcare Technology and Medicine 4(1):118 (2019)
  11. S. Lestari, Enzim Dan Respirasi Anaerob, Kementrian Pendidikan dan Kebudayaan RI (2016)
  12. Y.K. Risna, S. Harimurti, Wihandoyo, Widodo, Kurva Pertumbuhan Isolat Bakteri Asam Laktat Dari Saluran Pencernaan Itik Lokal Asal Aceh, Jurnal Peternakan Indonesia 24(1):1–7 (2022)
  13. Usman, Azizah, S. Kusmajadi, Pengaruh Konsentrasi Bakteri Asam Laktat Lactobacillus plantarum Dan Lactobacillus casei Terhadap Mutu Mikrobiologi Dan Kimia Mayones Probiotik, Jurnal Ilmu Ternak 18(2):17– 23 (2018)
  14. R. Anton, Bakteri Asam Laktat dan Mandai Cempedak, Mulawarman University Press (2019)
  15. A. Garcia, K. Navarro, E. Sanhueza, S. Pineda, E. Pastene, M. Quezada, K. Henriquez, A. Karlyshev, J. Villena, C. Gonzales, Charactrization of Lactobacillus fermentum UCO-979C, a Probiotic Strain with Anti- Helicobacterpylori Activity, Electron Journal Biotechnology 25:75–85 (2017)
  16. H. Rahmiati, A. Simanjuntak, Kemampuan Bakteri Asam Laktat Dalam Menghambat Salmonella thypii, Jurnal Jeumpa 6(2):257–64 (2019)
  17. Maarisit, Ismariani, Esther, D. Angkouw, E. P. M. Remy, D. C. R. Natalie, H. Manoppo, E.L. Ginting, Isolation and Antibacterial Activity Test of Seagrass Epiphytic Symbiont Bacteria Thalassia Hemprichii from Bahowo Waters, North Sulawesi, Jurnal Ilmiah PLATAX 9(1):115 (2021)
  18. M. Balaouri , M. Sadiki, S. K. Ibnsouda, Methods for In Viro Evaluating Antimicrobial Activity: A Review.” Journal of Pharmaceutical Analysis 6(2):71–79 (2016)
  19. H. Hidayat, W. Haryadi, S. Matsjeh, T.J. Raharjo, Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit, Biodiversitas Journal of Biological Diversity 20 (8) (2019)
  20. Nurhayati, L. Siti, N. Yahdiyani, A. Hidayatulloh, Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt Dengan Metode Difusi Sumuran Dan Metode Difusi Cakram, Jurnal Teknologi Hasil Peternakan 1(2):41–46 (2020)
  21. Widiani, P. Indri, K. J. P Pinatih, Uji Daya Hambat Ekstrak Etanol Daun Kelor (Moringa oleifera) Terhadap Pertumbuhan Bakteri Methicillin Resistant Staphylococcus aureus (MRSA), Jurnal Medika Udayana 9(3):22–28 (2020)
  22. Purwandari, Vivi, J.K. Marpaung, Suharyanisa, Uji Aktivitas Antibakteri Dari Black Garlic Dengan Variasi Waktu Berbeda Terhadap Streptococcus mutans Penyebab Karies Gigi, Farmanesia 8(2):100–106 (2021)
  23. Rijayanti, R. Pratiwi, S. Luliana, H. F. Trianto, Uji Aktivitas Antibakteri Ekstrak Etanol Daun Mangga Bacang, 13–14 (2014)