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Abstract
Salak seeds have been developed as a beverage, but there was still a little amount of research that focused on salak seeds. This research was conducted to find out the chemical compounds and the antioxidant activity of ethanolic extract and fraction of salak fruits seeds ( Salacca zalacca (Gaertn.) Voss. ) which have been grown extensively in Sleman Yogyakarta. Extraction was conducted using maceration, followed by fractionation using vacuum liquid chromatography. The identification of the chemical compounds contained in the ethanolic extract and fraction was performed by thin layer chromatography method, while the antioxidant activity was performed by DPPH method. Comparison of antioxidant activity was seen using IC50 values. The results showed that ethanol extract and fraction contained phenol, flavonoid, and tannin. The largest antioxidant activity was found in F7 with an IC50 value of 110.16 μg / ml.
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References
Adikristya, A., 2017, Kopi Biji Salak: Mencoba Sensasi Berbeda, Otten Magazine, 9 Maret 2017 didapatkan secara online di https://majalah.ottencoffee.co.id/kopi-biji-salak-mencoba-sensasi-berbeda/.
Alam Md. N., Bristi, N. J., and Rafiquzzaman, Md., 2012, Review on in vivo and in vitro methods evaluation of antioxidant activity, Saudi Pharmaceutical Journal (2013) 21, 143–152.
BPS, 2013, Statistik Hortikultura Daerah Istimewa Yogyakarta, Badan Pusat Statistik Provinsi Daerah Istimewa Yogyakarta , Yogyakarta.
Doughari, JH., 2012, Phytochemicals: Extraction Methods, Basic Structures and Mode Of Action As Potential Chemotherapeutic Agents, Department of Microbiology, School of Pure and Appliied Sciences, Federal University of Technology, Nigeria, p.1-32.
Harborne, J.B., 1984, Metode Fitokimia Penuntun Cara Modern Menganalisis Tumbuhan, ITB, Bandung, p.49,53,105,259.
Hyun, D., Hernandez, J. O., Mattson, M. P., de Cabo, R., 2006, The Plasma Membrane Redox System in Aging, Ageing Research Reviews, 5 (2006) 209–220.
Kinnula, V. L. and Crapo, J. D., 2004, Superoxide Dismutases in Malignant Cells and Human Tumors. Free Rad. Biol. & Med., Vol. 36, No. 6, pp. 718 – 744.
Oroian, M. and Escriche, I., 2015, Antioxidant : Characterization, natural sources, extraction, and analysis, Food Res. Int. , 74 (2015): 10-36.
Purwanto, N., Rismawati, E., and Sadiyah, E. R., 2015, Uji Sitotoksik Ekstrak Biji Salak (Salacca zalacca (Gaert.) Voss) dengan Menggunakan Metode Brine Shrimp Lethality Test (BSLT). Prosiding Penelitian SPeSIA Unisba 2015.
Puryono, RI., Puspitasari, E., Ningsih, IY., 2015, Uji Aktivitas Antioksidan dari Berbagai Varietas Ekstrak Buah Salak ( Salacca zalacca ( Gaertn.) Voss Varieties using DPPH, Artikel Ilmiah Hasil Penelitian Mahasiswa; 4-5.
Rukmana, HR., 2008, Bertanam Buah-buahan di Pekarangan, Kanisius, Yogyakarta, p.52.
Singh, R.P., Sharad, S., Kapur, S., 2004, Free radicals and oxidative stress in neurodegenerative diseases: relevance of dietary antioxidants. J Indian Acad Clin Med, ;5(3):219.
Singh, U. and Jialal, I., 2006, Oxidative Stress and Atherosclerosis, Pathophysiology 13 (2006) 129–142
Smith, M. A., Rottkamp, C. A., Nunomura, A., Raina, A. K., Perry, G.,
Oxidative stress in Alzheimer’s disease. Biochim. Biophys. Acta., 1502, 139–144.
Suhartono, E. and Setiawan, B.,2006, Radikal bebas, antioksidan dan penyakit, 1st ed, Pustaka Buana, Banjarbaru, p.13.
Winarsi, H., 2011, Antioksidan Alami dan Radikal Bebas Potensi dan Aplikasinya Dalam Kesehatan, Kanisius, Yogyakarta, p.19,32,78-81.