Article Sidebar

Submitted
August 7, 2017
Accepted
September 11, 2017
Published
September 13, 2017
Download
PDF
Statistic
Read Counter : 2955 Download : 1806

Main Article Content

Abstract

Salak seeds have been developed as a beverage, but there was still a little amount of research that focused on salak seeds. This research was conducted to find out the chemical compounds and the antioxidant activity of ethanolic extract and fraction of salak fruits seeds ( Salacca zalacca (Gaertn.) Voss. ) which have been grown extensively in Sleman Yogyakarta. Extraction was conducted using maceration, followed by fractionation using vacuum liquid chromatography. The identification of the chemical compounds contained in the ethanolic extract and fraction was performed by thin layer chromatography method, while the antioxidant activity was performed by DPPH method. Comparison of antioxidant activity was seen using IC50 values. The results showed that ethanol extract and fraction contained phenol, flavonoid, and tannin. The largest antioxidant activity was found in F7 with an IC50 value of 110.16 μg / ml.

Keywords

Antioxidant salak fruit seeds DPPH

Article Details

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Author Biography

Sista Werdyani, Department of Pharmacy, Universitas Islam Indonesia, Yogyakarta

Department of Pharmacy
How to Cite
Werdyani, S., Jumaryatno, P., & Khasanah, N. (2017). ANTIOXIDANT ACTIVITY OF ETHANOLIC EXTRACT AND FRACTION OF SALAK FRUIT SEEDS ( Salacca zalacca (Gaertn.) Voss. ) USING DPPH (2,2-diphenyl-1-picrylhydrazyl) METHOD. EKSAKTA: Journal of Sciences and Data Analysis, 17(2), 137–146. https://doi.org/10.20885/eksakta.vol17.iss2.art5
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

References

  1. Adikristya, A., 2017, Kopi Biji Salak: Mencoba Sensasi Berbeda, Otten Magazine, 9 Maret 2017 didapatkan secara online di https://majalah.ottencoffee.co.id/kopi-biji-salak-mencoba-sensasi-berbeda/.
  2. Alam Md. N., Bristi, N. J., and Rafiquzzaman, Md., 2012, Review on in vivo and in vitro methods evaluation of antioxidant activity, Saudi Pharmaceutical Journal (2013) 21, 143–152.
  3. BPS, 2013, Statistik Hortikultura Daerah Istimewa Yogyakarta, Badan Pusat Statistik Provinsi Daerah Istimewa Yogyakarta , Yogyakarta.
  4. Doughari, JH., 2012, Phytochemicals: Extraction Methods, Basic Structures and Mode Of Action As Potential Chemotherapeutic Agents, Department of Microbiology, School of Pure and Appliied Sciences, Federal University of Technology, Nigeria, p.1-32.
  5. Harborne, J.B., 1984, Metode Fitokimia Penuntun Cara Modern Menganalisis Tumbuhan, ITB, Bandung, p.49,53,105,259.
  6. Hyun, D., Hernandez, J. O., Mattson, M. P., de Cabo, R., 2006, The Plasma Membrane Redox System in Aging, Ageing Research Reviews, 5 (2006) 209–220.
  7. Kinnula, V. L. and Crapo, J. D., 2004, Superoxide Dismutases in Malignant Cells and Human Tumors. Free Rad. Biol. & Med., Vol. 36, No. 6, pp. 718 – 744.
  8. Oroian, M. and Escriche, I., 2015, Antioxidant : Characterization, natural sources, extraction, and analysis, Food Res. Int. , 74 (2015): 10-36.
  9. Purwanto, N., Rismawati, E., and Sadiyah, E. R., 2015, Uji Sitotoksik Ekstrak Biji Salak (Salacca zalacca (Gaert.) Voss) dengan Menggunakan Metode Brine Shrimp Lethality Test (BSLT). Prosiding Penelitian SPeSIA Unisba 2015.
  10. Puryono, RI., Puspitasari, E., Ningsih, IY., 2015, Uji Aktivitas Antioksidan dari Berbagai Varietas Ekstrak Buah Salak ( Salacca zalacca ( Gaertn.) Voss Varieties using DPPH, Artikel Ilmiah Hasil Penelitian Mahasiswa; 4-5.
  11. Rukmana, HR., 2008, Bertanam Buah-buahan di Pekarangan, Kanisius, Yogyakarta, p.52.
  12. Singh, R.P., Sharad, S., Kapur, S., 2004, Free radicals and oxidative stress in neurodegenerative diseases: relevance of dietary antioxidants. J Indian Acad Clin Med, ;5(3):219.
  13. Singh, U. and Jialal, I., 2006, Oxidative Stress and Atherosclerosis, Pathophysiology 13 (2006) 129–142
  14. Smith, M. A., Rottkamp, C. A., Nunomura, A., Raina, A. K., Perry, G.,
  15. Oxidative stress in Alzheimer’s disease. Biochim. Biophys. Acta., 1502, 139–144.
  16. Suhartono, E. and Setiawan, B.,2006, Radikal bebas, antioksidan dan penyakit, 1st ed, Pustaka Buana, Banjarbaru, p.13.
  17. Winarsi, H., 2011, Antioksidan Alami dan Radikal Bebas Potensi dan Aplikasinya Dalam Kesehatan, Kanisius, Yogyakarta, p.19,32,78-81.