Article Sidebar

cover JIF 18(1) 2022
Submitted
November 29, 2021
Accepted
July 28, 2022
Published
July 31, 2022
Download
PDF (Bahasa Indonesia)
Statistic
Read Counter : 580 Download : 773

Downloads

Download data is not yet available.

Main Article Content

Abstract

Background: Tuberculosis (TB) is a disease with a high mortality rate in the world, including in Indonesia. In addition, the problem of Mycobacterium tuberculosis resistance to antibiotics is getting higher. Mint leaves and garlic have been shown its the ability to kill M. tuberculosis. However, it has never been studied how the combined effect the M. tuberculosis growth.
Objective: The major objective of this study was to investigate the anti-mycobacterial activity of mint leaves-garlic combination to growth inhibition of clinical culture M. tuberculosis.
Method: Extraction of mint leaves and garlic was carried out by maceration using ethanol as a solvent. M. tuberculosis cultures were obtained from clinical samples of patients diagnosed with new tuberculosis (TBB) and MDR- tuberculosis (TBB). The growth inhibition activity test was carried out using the agar dilution method on Lowenstein Jensen (LJ) media.
Results: Mint and garlic extracts, and their combination can inhibit the growth of M. tuberculosis clinical culture from TBB and TBM patients with minimal inhibitory concentration (MIC) of 3 mg/mL.
Conclusion: Mint and garlic extracts, and their combination are effective in inhibiting the growth of M. tuberculosis clinical culture and can be used as an alternative treatment for tuberculosis disease.
Keywords: mint leaves (Mentha spicata), garlic (Allium sativum), Mycobacterium tuberculosis, multidrug-resistant (MDR)-Tuberculosis


Intisari
Latar belakang: Tuberkulosis (TBC) merupakan penyakit dengan tingkat kematian yang tinggi di dunia, termasuk Indonesia. Selain itu, masalah resistensi bakteri terhadap antibiotik semakin tinggi. Daun mint (Mentha spicata) dan bawang putih (Allium sativum) telah terbukti dapat membunuh bakteri M. tuberculosis. Namun belum pernah diteliti bagaimana efek kombinasi keduanya dalam membunuh atau menghambat pertumbuhan bakteri M. tuberculosis.
Tujuan: Mengetahui aktivitas kombinasi ekstrak daun mint dan bawang putih dalam menghambat pertumbuhan bakteri M. tuberculosis isolat klinik.
Metode: Ekstraksi daun mint dan bawang putih dilakukan secara maserasi menggunakan pelarut ethanol. Bakteri uji M. tuberculosis diperoleh dari sampel klinik pasien terdiagnosis tuberkulosis baru (TBB) dan tuberkulosis multidrug resistant (TBB). Uji aktivitas penghambatan pertumbuhan dilakukan menggunakan metode dilusi padat pada media Lowenstein Jensen (LJ).
Hasil: Ekstrak daun mint dan bawang putih serta kombinasi keduanya dapat menghambat pertumbuhan bakteri M. tuberculosis isolat klinik dari pasien TBB maupun TBM dengan konsentrasi terkecil 3 mg/mL.
Kesimpulan: Ekstrak daun mint dan bawang putih serta kombinasi keduanya efektif menghambat pertumbuhan bakteri M. tuberculosis isolat klinik dan berpotensi untuk digunakan sebagai alternatif pengobatan tuberkulosis.
Kata kunci: daun mint (Mentha spicata), bawang putih (Allium sativum), Mycobacterium tuberculosis, multidrug-resistant (MDR)-TB

Keywords

mint leaves Mentha spicata garlic Allium sativum Mycobacterium tuberculosis multidrug-resistant (MDR)-Tuberculosis

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC

References

  1. Batiha, G.E.S., Beshbishy, A.M., Wasef, L.G., Elewa, Y.H.A., Al-Sagan, A.A., El-Hack, M.E.A., dkk., 2020. Chemical constituents and pharmacological activities of garlic (Allium sativum L.): A review. Nutrients, 12: 1–21.
  2. Gigantesco, A. dan Giuliani, and M., 2011. The potential role of garlic (Allium sativum) against the multi-drug resistant tuberculosis pandemic: a review. Ann Ist Super Sanità, 47: 363–372.
  3. Hannan, A., Ullah, M.I., Usman, M., Hussain, S., Absar, M., dan Javed, K., 2011. Anti-mycobacterial activity of garlic (Allium sativum) against multi-drug resistant and non-multi-drug resistant Mycobacterium tuberculosis. Pakistan Journal of Pharmaceutical Sciences, 24: 81–85.
  4. Kristinawati, B., Muryadewi, A., dan Irianti, A.D., 2020. The role of family as a caregiver in caring for family members that are suffering from pulmonary tuberculosis. Jurnal Ners, 14: 362.
  5. Martins, N., Petropoulos, S., dan Ferreira, I.C.F.R., 2016. Chemical composition and bioactive compounds of garlic (Allium sativum L.) as affected by pre- and post-harvest conditions: a review. Food Chemistry, 211: 41–50.
  6. Muniyan, R. dan Jayaraman, G., 2016. Lauric acid and myristic acid from Allium sativum inhibit the growth of Mycobacterium tuberculosis H37Ra: in silico analysis reveals possible binding to protein kinase B. Pharmaceutical Biology, 54: 2814–2821.
  7. Nindrea, R.D., Sari, N.P., Harahap, W.A., Haryono, S.J., Kusnanto, H., Dwiprahasto, I., dkk., 2020. Survey data of multidrug-resistant tuberculosis, tuberculosis patients characteristics and stress resilience during COVID-19 pandemic in West Sumatera Province, Indonesia. Data in Brief, 32
  8. Park, C.H., Yeo, H.J., Baskar, T.B., Park, Y.E., Park, J.S., Lee, S.Y., dkk., 2019. In vitro antioxidant and antimicrobial properties of flower, leaf, and stem extracts of Korean mint. Antioxidants, 8: 1–12.
  9. Pusdatin-Kemenkes, 2018. Infodatin Tuberkulosis.
  10. Reuter, H., Koch, L., dan Lawson, L., 1996. Therapeutic effects and applications of garlic and its preparations, dalam: garlic. the science and therapeutic application of Allium sativum L. and related species. William and Wilkins, Baltimore.
  11. Sheeba, G., Gomathi, K.S., dan Citarasu, T., 2015. Anti-mycobacterial and phytochemical investigation of methanol extracts of few medicinal plants. Journal of Chemical and Pharmaceutical Sciences, 8: 480–486.
  12. Tamhid, H.A., Wahyuono, S., dan Hertiani, T., 2015. Aktivitas antibakteri senyawa isolat daun Mundu (Garcinia dulcis) terhadap bakteri patogen. Jurnal Ilmiah Farmasi, 11(2):30-37.