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Abstract
Abstract: Free radicals can cause cell damage such as premature aging. To protect, ward off and stabilize free radicals, antioxidant compounds can be used. Plants that can be used as a source of antioxidants are gotu kola (Centella asiatica (L.) Urban). The aim of this research is formulating a nanoemulsion of gotu kola leaves extract as an active ingredient for producing the antioxidant serum. The steps of this research include: (1) extraction by maceration and Microwave-Assisted Extraction (MAE); (2) extract characterization; (3) formulation of nanoemulsion using Self-Nanoemulsifying Drug Delivery System (SNEDDS) method; (4) nanoemulsion testing: stability test, antioxidant activity test, and irritation test, (5) nanoemulsion characterization which includes particle size, transmittance, pH, and viscosity. The results showed that: (1) yields of the maceration method and MAE were 14,60% and 17,54%; (2) antioxidant compounds in gotu kola leaves extract are squalene, kaempferol, asiaticoside; The IC50 of the maceration and MAE extract were 93,152 ppm and 80,365 ppm; (3) nanoemulsions were made in 3 formulas (0,1; 0,3; and 0,5 g) with fixed variables of capryol 90, tween 20, and PEG 400 (1,5; 2,5; and 1); (4) stability test showed that only F1 was stable; the IC50 value of nanoemulsion is 2604,967 ppm; and the F1 irritation test showed no erythema and edema; (5) The particle size of F1 is 166,7 nm with a transmittance value of 97.4%, a pH of 5,33, and a viscosity of 75,35 cP.
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References
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References
References
Rosaini, H., Makmur, I., Putri, R. D., & Sidoretno, W. M., Formulasi dan Pengujian Aktivitas Antioksidan dan Antibakteri Sediaan Masker Gel Peel Off Ekstrak Etanol Herba Seledri (Apium graveolens L .), Jurnal Farmasi Higea 11(2) (2019), 133–144.
Nurrosyidah, I. H., Aktivitas Antioksidan Ekstrak Etanol Herba Pegagan (Centella asiatica (L.) Urban) Dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil), Journal of Pharmaceutical Care Anwar Medika 3(1) (2020), 46–54.
Haliza, M. N., Aananti, W., & Santoso, J., Formulasi Sediaan Serum Spray Ekstrak Pegagan (Centella asiatica L.) Sebagai Anti Aging Alami, Ejournal Poltek Tegal (2020) 7(1), 1–6.
Cahya, A. P., and Fitri, N., Formulasi Dan Uji Antioksidan Serum Wajah Berbasis Minyak Jintan Hitam (Nigella Sativa L.) Menggunakan Metode Dpph. Asian Journal of Innovation and Entrepreneurship (2020) 05(03), 1–10.
Dini, Y., and Marleen, S. E. W., (2020). Aktivitas Antioksidan Daun Pegagan (Centella asiatica L.Urban) Dan Bunga Krisan (Crhysanthemum Sp) Pada Tiga Variasi Suhu Pengeringan, Pasundan Food Technology Journal, 6(3).
Sumiati, T., Effendy, F., & Riani, E. (2019). FORMULASI LOSION EKSTRAK HERBA PEGAGAN (Centella Asiatica (L.) Urban) DAN UJI MUTU SERTA STABILITASNYA. Jurnal Farmamedika (Pharmamedica Journal), 4(2), 62–69.
Kaul, S., Gulati, N., Verma, D., Mukherjee, S., and Nagaich, U., Role of Nanotechnology in Cosmeceuticals: A Review of Recent Advances, Journal of Pharmaceutics (2018) 1–19.
Setiawan, S.D., Ramadhani, C.C., Veronika, A., Ningrum, A.D.K., Nugroho, B.H., and Syukri, Y., 2018, Study of self nano-emulsifying drug delivery system (Snedds) loaded red fruit oil (pandanus conoideus lamk.) as an eliminated cancer cell mcf-7, International Journal of Drug Delivery Technology, 8 (4), 229–232.
Puspita, A. L., & Susilowati, S. (2021). Aktivitas Antioksidan Fraksi Daun Pegagan (Centella asiatica (L.) Urb.) dengan Metode FRAP Antioxidant Activity of Centella asiatica (L.) Urb. Leaves Fraction Using FRAP Method. Indonesian Journal on Medical Science, 8(2), 154–159.
Fitri, N., Halimatussa’diah, and Fitriastuti, D. (2019). Comparison between maceration and microwave extraction techniques of strawberry fruit (fragaria sp) and antioxidant activity test. IOP Conference Series: Materials Science and Engineering, 523(1).
Jatmiko, M. P., and Mursiti, (2021). Isolation, Identification, and Activity Test of Flavonoid Compounds in Jamblang Leaves (Syzygium cumini L.) Skeel as Antioxidants. Indonesia Journal of Chemical Science, 10(2), 129–138.
Zakaria, F., Ibrahim, W. N. W., Ismail, I. S., Ahmad, H., Manshoor, N., Ismail, N., Zainal, Z., & Shaari, K. (2019). LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of centella asiatica on zebrafish model. Pertanika Journal of Science and Technology, 27(2), 985–1003
Nugroho, B. H., and Sari, N. P. (2018). Formulation of Self Nano Emulsifiying Drug Delivery System (SNEDDS) Karamunting Leaf Extract (Rhodomyrtus tomentosa (Ait.) Hassk). Jurnal Ilmiah Farmasi, 14(1), 1–8.
Lestari, R. (2018). Optimasi Pembuatan Nanopartikel Ekstrak Limbah Kulit Kakao ( Theobroma cacao L . ) Sebagai Antibakteri Terhadap Klebsiella pneumonia, Skripsi, FMIPA UII.