Main Article Content
Abstract
This study aims to standardize the specific and non-specific parameters of propolis extract originating from East Java, Indonesia, to fulfil the requirements as a herbal raw material. Standardization was carried out on propolis ethanol extract taken at three different harvesting times. Standardization was carried out on specific parameters including the content of dissolved compounds, chemical content of extracts and chromatogram patterns, while the non-specific parameters of the extract included water content, ash content residue, density, microbial contamination, and heavy metal contamination. Specific extract parameters showed that the water-soluble extract content was 2.1-3.5%; levels of ethanol-soluble extract 62.00-84.00%; total flavonoid levels of 0.015-0.072 mg ER / g; and total phenol content of 0.0039-0.0053 mg ER / g. Non-specific parameters indicate that the water content is 10.72-10.90%; drying losses 10.05-10.63%; total ash content of 0.08-0.65%, density of 0.88-0.89 g / mL; Pb levels from 6.55-9.01 mg / kg; Cd content of 0.50-1.22 mg / kg, Cu content of 0.82-1.13 mg / kg; the total plate number is 10 colonies / g and the yeast fungus number is 10 colonies / g. It can be concluded that propolis extract from the East Java region of Indonesia fulfills the requirements as a raw material for herbal products in Indonesia.
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References
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- Anonymous, general Standard parameters of Drug Plant Extract,Department of Health Republic of Indonesia, Jakarta, 2000
- S. K. Dinakaran,S. Chelle, H. Avasarala. Profiling and Determinaion of Phenolic Compounds in Poly Herbal Formulations and Their Comparative Evaluation, Journal of Traditional and Complementary Medicine 9 (4) (2019), 319-327
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References
J.M. Sforcin,V. Bankova, Propolis: Is Therea Potential for the Development of New Drugs? Journal of Ethnopharmacology 133 (2) (2011), 253-260
M.V. M das Neves, T. M. S da Silva, O. de E. Lima, E. V. L da Cunha, E. de J Oliveira, Isoflavone Formononetin from Red Propolis Acts as a Fungicide against Candida Sp. Braz., Journal of Microbiology 47 (1) (2016) , 159–166.
S. Almutairi, R. Edrada-Ebel, J. Fearnley, J. O. Igoli, W. Alotaibi, C. J. Clements, A. I Gray, D. G Watson, Isolation of Diterpenes and Flavonoids from a New Type of Propolis from Saudi Arabia, Phytochemistry Letter 10 (2014) , 160–163.
G. Valenzuela-Barra, C. Castro, C. Figueroa, A. Barriga, X. Silva, B. de las Heras, S. Hortelano, C. Delporte, Anti-Inflammatory Activity and Phenolic Profile of Propolis from Two Locations in Región Metropolitana de Santiago, Chile, Journal of Ethnopharmacology 168 (2015) , 37–44.
K. Wang, L. Hu, X.L.Jin, Q. X. Ma, M. C Marcucci, A. a. L Netto, A. C. H. F. Sawaya, S. Huang, W. K Ren, M. A. Conlon: et al., Polyphenol_Rich Propolis Extracts from China and Brazil Exert Anti-Inflammatory Effect by Modulating Ubiquitination of TRAF6 during the Activation of NF-kB, Journal of Functional Foods 19 (2015), 464-478
N. Paulino, S. R. L. Abreu, Y. Uto, D. Koyama, H. Nagasawa, H. Hori, V.M. Dirsch, A. M. Vollmar, A. Scremin, W. A. Bretz, Anti-Inflammatory Effects of a Bioavailable Compound, Artepillin C, in Brazilian Propolis, European Jounal Pharmacology 587 (1–3) (2008), 296–301.
D. A. Sampietro, M. M Sampietro Vattuone, M. A Vattuone. Immunomodulatory Activity of Apis Mellifera Propolis from the North of Argentina, LWT - Food Science and Technology 70 (2016), 9-15.
G. Fischer, G, F. R. Conceição, F. P. L. Leite, L. A. Dummer, G. D. Vargas, S. de O Hübner, O. A. Dellagostin, N. Paulino, A. S. Paulino, T. Vidor, Immunomodulation Produced by a Green Propolis
Extract on Humoral and Cellular Responses of Mice Immunized with SuHV-1, Vaccine, 25 (7) (2007), 1250–1256.
Z. Hasan. Antibacterial Activity of Propolis Trigona Spp. from Bukittinggi West Sumatera Against Salmonella Sp, Chemistry Progress 4 (2) (2011).
R. A. Laskar, N. Roy, N. A. Begum, Antioxidant Activity of Indian Propolis and Its Chemical Constituents, Food Chemistry 122 (1) (2010) , 233–237.
L. A. Elissa, N. M. Elsherbiny, A. O. Magmomah, Propolis Restored Adipenictin Level in Type 2 Diabetes through PPARy Activation, Egyption Journal of Basic And Applied Science 2 (4) (2015),318-326
S. M. Oses, A. Pascual-Mate, M. A. Fernandez-Muino, T.M. Lope-Diaz, M.T.Sancho, Bioactive Properties of Honey with Propolis Food Chemistry 196 (2016),1215-1223
I. A. Freires, S. M. de Alencar, P. L. A. Rosalen, Pharmacological Perspective on the use of Brazilian Red Propolis and Its Isolated Compound against Human Diseases, European Journal of Medicinal Chemistry 110(2016), 267-279
K. Graikou, M. Popova, O. Gortzi, V. Bnkova, I. Chinou, Characterization and Biological Evalution of Selected Mediterrranean Propolis Samples. Is it a New Type? LWT-Food Science and Technology 65 (20160,261-267
Anonymous, general Standard parameters of Drug Plant Extract,Department of Health Republic of Indonesia, Jakarta, 2000
S. K. Dinakaran,S. Chelle, H. Avasarala. Profiling and Determinaion of Phenolic Compounds in Poly Herbal Formulations and Their Comparative Evaluation, Journal of Traditional and Complementary Medicine 9 (4) (2019), 319-327
A. Nansak, V. Chabra, R. K. Rawal, S. Sharma,Chemometrics: A new Scenarion in Herbal Drug Standardization, Journal of Pharmaceutical Analysis 4 (4) (2014), 223-233
Z. Alfian, Analisis Kadar Logam Kadmium (Cd2+) Dari Kerang Yang Diperoleh Dari Daerah Belawan Secara Spektrofotometer Serapan Atom. Jurnal Sains dan Kimia 9 (2) (2005), 73-76
Anonim, Peraturan Kepala badan pengawas Obat dan Makanan Republik Indonesia Nomor 12 Tahun 2014 Tentang Label Gizi, BPOM Republik Indonesia, Jakarta,2016
Anonim, Batas Maksimum Cemaran Logam Berat Dalam Pangan, SNI 7389; 2009