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Abstract
This study investigated the effect of Guided Inquiry (GI) on pre-service teachers' academic achievement and retention in photosynthesis. A quasi-experimental research design was used with 65 pre-service teachers, where 32 were assigned to the experimental group (EG) and 33 to the control group (CG). Data were collected using a Learners' Achievement Test and analysed using descriptive statistics: means and standard deviations, and inferential statistics, including T-tests and analysis of covariance (ANCOVA). Both groups completed a pretest, and no significant difference was recorded in prior knowledge (p > .05). Post-tests measured pre-service teachers' achievement and retention after teaching both groups. A T-tests showed that learners taught using GI scored significantly higher than those taught using Traditional Teaching (TT) (p < .05). ANCOVA, controlling pretest scores, confirmed a significant effect of the teaching approach on post-test achievements in favour of GI (p < .05). Differences between male and female achievements were not statistically significant (p > .05). The findings suggest that GI enhances conceptual understanding and retention in science education.
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References
- D. D. Minner, A. J. Levy, and J. Century, “Inquiry-based science instruction—What is it and does it matter?” J. Res. Sci. Teach., vol. 47, no. 4, pp. 474–496, 2010, doi: 10.1002/tea.20347 DOI: https://doi.org/10.1002/tea.20347
- E. M. Furtak, T. Seidel, H. Iverson, and D. C. Briggs, “Experimental and quasi-experimental studies of inquiry-based science teaching: A meta-analysis,” Rev. Educ. Res., vol. 82, no. 3, pp. 300–329, 2012, doi: 10.3102/0034654312457206 DOI: https://doi.org/10.3102/0034654312457206
- A. Çimer, “What makes biology learning difficult and effective: Students’ views,” Educ. Res. Rev., vol. 7, no. 3, pp. 61–71, 2012. [Online]. Available: https://journal-backups.lon1.digitaloceanspaces.com/uploads/main/article/article1379665422_Cimer.pdf.
- R. E. Mayer, Applying the Science of Learning. Pearson, 2011. DOI: https://doi.org/10.1016/B978-0-12-387691-1.00003-X
- J. Liu et al., “Biodegradation of the fungicide picoxystrobin by Hyphomicrobium sp. H-9 and detoxification mechanism,” J. Agric. Food Chem., vol. 73, no. 15, pp. 9221–9233, 2025, doi: 10.1021/acs.jafc.5c00652 DOI: https://doi.org/10.1021/acs.jafc.5c00652
- A. W. Lazonder and R. Harmsen, “Meta-analysis of inquiry-based learning: Effects of guidance,” Rev. Educ. Res., vol. 86, no. 3, pp. 681–718, 2016, doi: 10.3102/0034654315627366. DOI: https://doi.org/10.3102/0034654315627366
- C. E. Hmelo-Silver, R. G. Duncan, and C. A. Chinn, “Scaffolding and achievement in problem-based and inquiry learning,” Educ. Psychol., vol. 42, no. 2, pp. 99–107, 2007, doi: 10.1080/00461520701263368 DOI: https://doi.org/10.1080/00461520701263368
- U. Ramnarain and M. Modiba, “Teachers’ perceptions of inquiry-based learning in rural science classrooms,” S. Afr. J. Educ., vol. 33, no. 3, pp. 1–10, 2013. 10.15700/saje.v33n3a807
- D. Mekonen and A. D. Kelkay, “Inquiry-based instructional strategies for effective conceptualization of photosynthesis,” Cogent Educ., vol. 10, no. 1, Art. no. 2172927, 2023. 10.1080/2331186X.2023.2172927 DOI: https://doi.org/10.1080/2331186X.2023.2172927
- W. Gayus, A. A. Mohammed, A. Shuaibu, and N. Naye, “Effects of analogy and guided inquiry instructional strategies on academic achievement,” Fed. Univ. Gusau Fac. Educ. J., vol. 3, no. 1, pp. 228–236, 2026. 10.64348/zije.2026363 DOI: https://doi.org/10.64348/zije.2026363
- K. Jančaříková and A. Jančařík, “How to teach photosynthesis? A review of academic research,” Sustainability, vol. 14, no. 20, Art. no. 13529, 2022. 10.3390/su142013529 DOI: https://doi.org/10.3390/su142013529
- M. Danil, A. D. Corebima, S. Mahanal, and Ibrohim, “The connection between students’ retention and critical thinking skills,” J. Pendidik. IPA Indones., vol. 12, no. 2, pp. 241–251, 2023. 10.15294/jpii.v12i2.39983 DOI: https://doi.org/10.15294/jpii.v12i2.39983
- M. Z. Kamarudin and M. S. A. M. Noor, “Teacher’s practice in digital inquiry-based science learning,” J. Sci. Learn. Innov., vol. 1, no. 1, pp. 33–60, 2024. 10.1163/29497736-bja00004 DOI: https://doi.org/10.1163/29497736-bja00004
- A. O. Owolade, M. O. Salami, A. O. Kareem, and P. O. Oladipupo, “Effectiveness of guided inquiry and open inquiry instructional strategies,” Anatol. J. Educ., vol. 7, no. 2, pp. 19–30, 2022. 10.29333/aje.2022.723a
- L. S. Vygotsky, Mind in Society: The Development of Higher Psychological Processes. Harvard Univ. Press, 1978.
- UNESCO, Inclusive Science Education and Gender Equality in STEM. UNESCO Publ., 2020.
- T. D. Cook, D. T. Campbell, and W. R. Shadish, Experimental and Quasi-Experimental Designs for Generalized Causal Inference. Houghton Mifflin, 2002.
- D. T. Campbell and J. C. Stanley, Experimental and Quasi-Experimental Designs for Research. Houghton Mifflin, 1963.
- A. Bryman, Social Research Methods, 5th ed. Oxford Univ. Press, 2016.
- J. W. Creswell and J. D. Creswell, Research Design: Qualitative, Quantitative, and Mixed Methods Approaches. Sage, 2018.
- J. C. Nunnally and I. H. Bernstein, Psychometric Theory, 3rd ed. McGraw-Hill, 1994.
- K. S. Taber, “The use of Cronbach’s alpha when developing and reporting research instruments,” Res. Sci. Educ., vol. 48, no. 6, pp. 1273–1296, 2018. 10.1007/s11165-016-9602-2 DOI: https://doi.org/10.1007/s11165-016-9602-2
- R. Geier et al., “Standardized test outcomes for students engaged in inquiry-based science curricula,” J. Res. Sci. Teach., vol. 45, no. 8, pp. 922–939, 2008. 10.1002/tea.20248 DOI: https://doi.org/10.1002/tea.20248
- M. Prince, “Does active learning work? A review of the research,” J. Eng. Educ., vol. 93, no. 3, pp. 223–231, 2004. 10.1002/j.2168-9830.2004.tb00809.x DOI: https://doi.org/10.1002/j.2168-9830.2004.tb00809.x
- S. Freeman et al., “Active learning increases student performance,” Proc. Natl. Acad. Sci. U.S.A., vol. 111, no. 23, pp. 8410–8415, 2014, doi: 10.1073/pnas.1319030111 DOI: https://doi.org/10.1073/pnas.1319030111
- E. J. Theobald et al., “Active learning narrows achievement gaps,” Proc. Natl. Acad. Sci. U.S.A., vol. 117, no. 12, pp. 6476–6483, 2020, doi: 10.1073/pnas.1916903117 DOI: https://doi.org/10.1073/pnas.1916903117
- C. C. Kuhlthau, The Library Research Process. Ablex Publ., 1985.
- A. Field, Discovering Statistics Using IBM SPSS Statistics, 5th ed. Sage, 2018.
- A. Aditomo and E. Klieme, “Forms of inquiry-based science instruction,” Int. J. Sci. Educ., vol. 42, no. 4, pp. 504–525, 2020, doi:` 10.1080/09500693.2020.1716093 DOI: https://doi.org/10.1080/09500693.2020.1716093
- P. H. Chikaluma, D. Opanga, and V. Nsengimana, “Contribution of inquiry-based learning to biology teaching,” Int. J. Sci. Math. Technol. Learn., vol. 29, no. 2, p. 29, 2022, doi: 10.18848/2327-7971/CGP/v29i02/29-52 DOI: https://doi.org/10.18848/2327-7971/CGP/v29i02/29-52
- M. Kersting et al., “Quality of inquiry-based teaching in science classrooms,” Int. J. Sci. Educ., vol. 45, no. 17, pp. 1463–1484, 2023, doi: 10.1080/09500693.2023.2213386 DOI: https://doi.org/10.1080/09500693.2023.2213386
- S. Areepattamannil, “Guided inquiry in school science: a mini review of orchestration, assessment, and AI,” Front. Educ., vol. 10, Art. no. 1534358, 2025, doi: 10.3389/feduc.2025.1534358. DOI: https://doi.org/10.3389/feduc.2025.1534358
- R. L. Gómez and A. M. Suárez, “Do inquiry-based teaching and school climate influence science achievement and critical thinking? Evidence from PISA 2015,” Int. J. STEM Educ., vol. 7, Art. no. 43, 2020, doi: 10.1186/s40594-020-00240-5 DOI: https://doi.org/10.1186/s40594-020-00240-5
- A. Skulmowski and K. M. Xu, “Understanding cognitive load in digital and online learning: A new perspective on extraneous cognitive load,” Educ. Psychol. Rev., vol. 34, no. 1, pp. 171–196, 2022, doi: 10.1007/s10648-021-09624-7 DOI: https://doi.org/10.1007/s10648-021-09624-7
- P. A. Kirschner, J. Sweller, and R. E. Clark, “Why minimal guidance during instruction does not work,” Educ. Psychol., vol. 41, no. 2, pp. 75–86, 2006, doi: 10.1207/s15326985ep4102_1 DOI: https://doi.org/10.1207/s15326985ep4102_1
- I. Adler and L. Sarsour, “Teacher’s guidance in inquiry-based environments,” Instr. Sci., vol. 52, no. 3, pp. 453–475, 2024. 10.1007/s11251-023-09649-1 DOI: https://doi.org/10.1007/s11251-023-09649-1
- J. Cohen, Statistical Power Analysis for the Behavioral Sciences, 2nd ed. Lawrence Erlbaum Assoc., 1988.
- S. A. Hussain, F. Ayub, N. Ahmed, and Ziauddin, “Cognitive load management through adaptive AI learning system,” Crit. Rev. Soc. Sci. Stud., vol. 3, no. 3, pp. 701–719, 2025, doi: 10.59075/kpfrdv65 DOI: https://doi.org/10.59075/kpfrdv65
- T. De Jong and A. W. Lazonder, “The guided discovery learning principle in multimedia learning,” in The Cambridge Handbook of Multimedia Learning, 2nd ed., R. E. Mayer, Ed. Cambridge, U.K.: Cambridge University Press, 2014, pp. 371–390, doi: 10.1017/CBO9781139547369.019. DOI: https://doi.org/10.1017/CBO9781139547369.019
- E. Silfver, “Gender performance in an out-of-school science context,” Cult. Stud. Sci. Educ., vol. 14, pp. 139–155, 2019, doi: 10.1007/s11422-017-9851-z DOI: https://doi.org/10.1007/s11422-017-9851-z
- K. Bussey and A. Bandura, “Social cognitive theory of gender development,” Psychol. Rev., vol. 106, no. 4, pp. 676–713, 1999, doi: 10.1037/0033-295X.106.4.676. DOI: https://doi.org/10.1037/0033-295X.106.4.676
- National Research Council, A Framework for K–12 Science Education. Washington, DC, USA: National Academies Press, 2012, pp. 1–400.
References
D. D. Minner, A. J. Levy, and J. Century, “Inquiry-based science instruction—What is it and does it matter?” J. Res. Sci. Teach., vol. 47, no. 4, pp. 474–496, 2010, doi: 10.1002/tea.20347 DOI: https://doi.org/10.1002/tea.20347
E. M. Furtak, T. Seidel, H. Iverson, and D. C. Briggs, “Experimental and quasi-experimental studies of inquiry-based science teaching: A meta-analysis,” Rev. Educ. Res., vol. 82, no. 3, pp. 300–329, 2012, doi: 10.3102/0034654312457206 DOI: https://doi.org/10.3102/0034654312457206
A. Çimer, “What makes biology learning difficult and effective: Students’ views,” Educ. Res. Rev., vol. 7, no. 3, pp. 61–71, 2012. [Online]. Available: https://journal-backups.lon1.digitaloceanspaces.com/uploads/main/article/article1379665422_Cimer.pdf.
R. E. Mayer, Applying the Science of Learning. Pearson, 2011. DOI: https://doi.org/10.1016/B978-0-12-387691-1.00003-X
J. Liu et al., “Biodegradation of the fungicide picoxystrobin by Hyphomicrobium sp. H-9 and detoxification mechanism,” J. Agric. Food Chem., vol. 73, no. 15, pp. 9221–9233, 2025, doi: 10.1021/acs.jafc.5c00652 DOI: https://doi.org/10.1021/acs.jafc.5c00652
A. W. Lazonder and R. Harmsen, “Meta-analysis of inquiry-based learning: Effects of guidance,” Rev. Educ. Res., vol. 86, no. 3, pp. 681–718, 2016, doi: 10.3102/0034654315627366. DOI: https://doi.org/10.3102/0034654315627366
C. E. Hmelo-Silver, R. G. Duncan, and C. A. Chinn, “Scaffolding and achievement in problem-based and inquiry learning,” Educ. Psychol., vol. 42, no. 2, pp. 99–107, 2007, doi: 10.1080/00461520701263368 DOI: https://doi.org/10.1080/00461520701263368
U. Ramnarain and M. Modiba, “Teachers’ perceptions of inquiry-based learning in rural science classrooms,” S. Afr. J. Educ., vol. 33, no. 3, pp. 1–10, 2013. 10.15700/saje.v33n3a807
D. Mekonen and A. D. Kelkay, “Inquiry-based instructional strategies for effective conceptualization of photosynthesis,” Cogent Educ., vol. 10, no. 1, Art. no. 2172927, 2023. 10.1080/2331186X.2023.2172927 DOI: https://doi.org/10.1080/2331186X.2023.2172927
W. Gayus, A. A. Mohammed, A. Shuaibu, and N. Naye, “Effects of analogy and guided inquiry instructional strategies on academic achievement,” Fed. Univ. Gusau Fac. Educ. J., vol. 3, no. 1, pp. 228–236, 2026. 10.64348/zije.2026363 DOI: https://doi.org/10.64348/zije.2026363
K. Jančaříková and A. Jančařík, “How to teach photosynthesis? A review of academic research,” Sustainability, vol. 14, no. 20, Art. no. 13529, 2022. 10.3390/su142013529 DOI: https://doi.org/10.3390/su142013529
M. Danil, A. D. Corebima, S. Mahanal, and Ibrohim, “The connection between students’ retention and critical thinking skills,” J. Pendidik. IPA Indones., vol. 12, no. 2, pp. 241–251, 2023. 10.15294/jpii.v12i2.39983 DOI: https://doi.org/10.15294/jpii.v12i2.39983
M. Z. Kamarudin and M. S. A. M. Noor, “Teacher’s practice in digital inquiry-based science learning,” J. Sci. Learn. Innov., vol. 1, no. 1, pp. 33–60, 2024. 10.1163/29497736-bja00004 DOI: https://doi.org/10.1163/29497736-bja00004
A. O. Owolade, M. O. Salami, A. O. Kareem, and P. O. Oladipupo, “Effectiveness of guided inquiry and open inquiry instructional strategies,” Anatol. J. Educ., vol. 7, no. 2, pp. 19–30, 2022. 10.29333/aje.2022.723a
L. S. Vygotsky, Mind in Society: The Development of Higher Psychological Processes. Harvard Univ. Press, 1978.
UNESCO, Inclusive Science Education and Gender Equality in STEM. UNESCO Publ., 2020.
T. D. Cook, D. T. Campbell, and W. R. Shadish, Experimental and Quasi-Experimental Designs for Generalized Causal Inference. Houghton Mifflin, 2002.
D. T. Campbell and J. C. Stanley, Experimental and Quasi-Experimental Designs for Research. Houghton Mifflin, 1963.
A. Bryman, Social Research Methods, 5th ed. Oxford Univ. Press, 2016.
J. W. Creswell and J. D. Creswell, Research Design: Qualitative, Quantitative, and Mixed Methods Approaches. Sage, 2018.
J. C. Nunnally and I. H. Bernstein, Psychometric Theory, 3rd ed. McGraw-Hill, 1994.
K. S. Taber, “The use of Cronbach’s alpha when developing and reporting research instruments,” Res. Sci. Educ., vol. 48, no. 6, pp. 1273–1296, 2018. 10.1007/s11165-016-9602-2 DOI: https://doi.org/10.1007/s11165-016-9602-2
R. Geier et al., “Standardized test outcomes for students engaged in inquiry-based science curricula,” J. Res. Sci. Teach., vol. 45, no. 8, pp. 922–939, 2008. 10.1002/tea.20248 DOI: https://doi.org/10.1002/tea.20248
M. Prince, “Does active learning work? A review of the research,” J. Eng. Educ., vol. 93, no. 3, pp. 223–231, 2004. 10.1002/j.2168-9830.2004.tb00809.x DOI: https://doi.org/10.1002/j.2168-9830.2004.tb00809.x
S. Freeman et al., “Active learning increases student performance,” Proc. Natl. Acad. Sci. U.S.A., vol. 111, no. 23, pp. 8410–8415, 2014, doi: 10.1073/pnas.1319030111 DOI: https://doi.org/10.1073/pnas.1319030111
E. J. Theobald et al., “Active learning narrows achievement gaps,” Proc. Natl. Acad. Sci. U.S.A., vol. 117, no. 12, pp. 6476–6483, 2020, doi: 10.1073/pnas.1916903117 DOI: https://doi.org/10.1073/pnas.1916903117
C. C. Kuhlthau, The Library Research Process. Ablex Publ., 1985.
A. Field, Discovering Statistics Using IBM SPSS Statistics, 5th ed. Sage, 2018.
A. Aditomo and E. Klieme, “Forms of inquiry-based science instruction,” Int. J. Sci. Educ., vol. 42, no. 4, pp. 504–525, 2020, doi:` 10.1080/09500693.2020.1716093 DOI: https://doi.org/10.1080/09500693.2020.1716093
P. H. Chikaluma, D. Opanga, and V. Nsengimana, “Contribution of inquiry-based learning to biology teaching,” Int. J. Sci. Math. Technol. Learn., vol. 29, no. 2, p. 29, 2022, doi: 10.18848/2327-7971/CGP/v29i02/29-52 DOI: https://doi.org/10.18848/2327-7971/CGP/v29i02/29-52
M. Kersting et al., “Quality of inquiry-based teaching in science classrooms,” Int. J. Sci. Educ., vol. 45, no. 17, pp. 1463–1484, 2023, doi: 10.1080/09500693.2023.2213386 DOI: https://doi.org/10.1080/09500693.2023.2213386
S. Areepattamannil, “Guided inquiry in school science: a mini review of orchestration, assessment, and AI,” Front. Educ., vol. 10, Art. no. 1534358, 2025, doi: 10.3389/feduc.2025.1534358. DOI: https://doi.org/10.3389/feduc.2025.1534358
R. L. Gómez and A. M. Suárez, “Do inquiry-based teaching and school climate influence science achievement and critical thinking? Evidence from PISA 2015,” Int. J. STEM Educ., vol. 7, Art. no. 43, 2020, doi: 10.1186/s40594-020-00240-5 DOI: https://doi.org/10.1186/s40594-020-00240-5
A. Skulmowski and K. M. Xu, “Understanding cognitive load in digital and online learning: A new perspective on extraneous cognitive load,” Educ. Psychol. Rev., vol. 34, no. 1, pp. 171–196, 2022, doi: 10.1007/s10648-021-09624-7 DOI: https://doi.org/10.1007/s10648-021-09624-7
P. A. Kirschner, J. Sweller, and R. E. Clark, “Why minimal guidance during instruction does not work,” Educ. Psychol., vol. 41, no. 2, pp. 75–86, 2006, doi: 10.1207/s15326985ep4102_1 DOI: https://doi.org/10.1207/s15326985ep4102_1
I. Adler and L. Sarsour, “Teacher’s guidance in inquiry-based environments,” Instr. Sci., vol. 52, no. 3, pp. 453–475, 2024. 10.1007/s11251-023-09649-1 DOI: https://doi.org/10.1007/s11251-023-09649-1
J. Cohen, Statistical Power Analysis for the Behavioral Sciences, 2nd ed. Lawrence Erlbaum Assoc., 1988.
S. A. Hussain, F. Ayub, N. Ahmed, and Ziauddin, “Cognitive load management through adaptive AI learning system,” Crit. Rev. Soc. Sci. Stud., vol. 3, no. 3, pp. 701–719, 2025, doi: 10.59075/kpfrdv65 DOI: https://doi.org/10.59075/kpfrdv65
T. De Jong and A. W. Lazonder, “The guided discovery learning principle in multimedia learning,” in The Cambridge Handbook of Multimedia Learning, 2nd ed., R. E. Mayer, Ed. Cambridge, U.K.: Cambridge University Press, 2014, pp. 371–390, doi: 10.1017/CBO9781139547369.019. DOI: https://doi.org/10.1017/CBO9781139547369.019
E. Silfver, “Gender performance in an out-of-school science context,” Cult. Stud. Sci. Educ., vol. 14, pp. 139–155, 2019, doi: 10.1007/s11422-017-9851-z DOI: https://doi.org/10.1007/s11422-017-9851-z
K. Bussey and A. Bandura, “Social cognitive theory of gender development,” Psychol. Rev., vol. 106, no. 4, pp. 676–713, 1999, doi: 10.1037/0033-295X.106.4.676. DOI: https://doi.org/10.1037/0033-295X.106.4.676
National Research Council, A Framework for K–12 Science Education. Washington, DC, USA: National Academies Press, 2012, pp. 1–400.