UJI KEPEKAAN BAKTERI Escherichia coli HASIL ISOLASI DARI URIN PASIEN RUMAH SAKIT Dr. Sardjito TERHADAP ANTIBIOTIK GOLONGAN ï¢-LAKTAM
Uncontrolled use of antibiotic caused the emergence of bacteria strain resistant toward ofÂ antibiotic. The sensitivity assay of E. coli toward ï¢-lactam antibiotic has been done. The sensitivityÂ assay was started with isolated bacteria from patients urine at Dr. Sardjito hospital followed byÂ count total of colony/number of germ which growth and identified the bacteria. The Kirby BauerÂ method was performed. Antibiotic impregnated disk (ampicillin 30 ïg, sulbactam/ampicillin 20 ïg,Â cefotaxim 30 ïg, ceftazidime 30 ïg, ceftriaxon 30 ïg, cefpirom 30 ïg, cefepim 30 ïg and imipenemÂ 10 ïg) were placed on agar plate previously streaked with suspension of E. coli (1.10Â 8Â CFU/ml).Â The plates were incubated for 18-24 hours at 37Â 0Â C. The diameters of the zone inhibition wereÂ measured, and compared to standar interpretive zone sizes. The result of the study showed that allÂ of E. coli resistant toward antibiotic ampicillin; 65% resistant toward antibiotic sulbactam/ampicillin;Â 50% still sensitive toward antibiotic third cefalosporin generation that is cefotaxim, ceftazidime,Â ceftriaxon; more than 50% sensitive toward fourth cefalosporin generation like cefepim, cefpiromÂ and all of pathogen bacteria E. coli sensitive toward antibiotic imipenem.
Key Words : E. coli, ï¢-lactam antibiotic, Sensitivity assay, Urine of patient hospital.
Jurnal Ilmiah Farmasi (JIF)
Department of Pharmacy, Faculty of Mathematics and Science,
Universitas Islam Indonesia.
JIF at http://journal.uii.ac.id/JIF/ is licensed under a Creative Commons Attribution 4.0 International License.