UJI KEPEKAAN BAKTERI Escherichia coli HASIL ISOLASI DARI URIN PASIEN RUMAH SAKIT Dr. Sardjito TERHADAP ANTIBIOTIK GOLONGAN ï¢-LAKTAM

Sri harnanik, Sri Mulyaningsih, Asih Triastuti

Abstract

ABSTRACT
Uncontrolled use of antibiotic caused the emergence of bacteria strain resistant toward of antibiotic. The sensitivity assay of E. coli toward ï¢-lactam antibiotic has been done. The sensitivity assay was started with isolated bacteria from patients urine at Dr. Sardjito hospital followed by count total of colony/number of germ which growth and identified the bacteria. The Kirby Bauer method was performed. Antibiotic impregnated disk (ampicillin 30 ï­g, sulbactam/ampicillin 20 ï­g, cefotaxim 30 ï­g, ceftazidime 30 ï­g, ceftriaxon 30 ï­g, cefpirom 30 ï­g, cefepim 30 ï­g and imipenem 10 ï­g) were placed on agar plate previously streaked with suspension of E. coli (1.10 8 CFU/ml). The plates were incubated for 18-24 hours at 37 0 C. The diameters of the zone inhibition were measured, and compared to standar interpretive zone sizes. The result of the study showed that all of E. coli resistant toward antibiotic ampicillin; 65% resistant toward antibiotic sulbactam/ampicillin; 50% still sensitive toward antibiotic third cefalosporin generation that is cefotaxim, ceftazidime, ceftriaxon; more than 50% sensitive toward fourth cefalosporin generation like cefepim, cefpirom and all of pathogen bacteria E. coli sensitive toward antibiotic imipenem.

Key Words : E. coli, ï¢-lactam antibiotic, Sensitivity assay, Urine of patient hospital.

Keywords

E. coli, -lactam antibiotic, Sensitivity assay, Urine of patient hospital.

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Jurnal Ilmiah Farmasi (JIF)
Published by:
Department of Pharmacy, Faculty of Mathematics and Science,
Universitas Islam Indonesia.

ISSN 1693-8666

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