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Abstract
ABSTRACT
A tissue culture and plant generation for the ceplukan, Physalis angulata, L has been
developed. Callus were efficiently induced on Murashige and Skoog (MS) medium supplemented
with 0,1 ppm indole-3-acetic acid (IAA) and variation of 1, 2, 3, 4, and 5 ppm benzyladenine (BA).
The regenerable callus differentiated into shoots. Phytochemical containing of the shoots were
detected and compared with the origin plant using Thin Layer Chromatography (TLC). Flavonoid
was showed by bright blue spot under UV 366 nm and became yellow after smoked by NH3 with
HRf 43 and 69. Saponin was showed by yellow spot with HRf 18 and purple spot with HRf 45 after
sprayed by anisaldehyde-sulfuric acid. HRf of the shoots from culture and origin plant were the
same, showed that shoots contains the same phytochemical as the origin plant.
Keyword: shoots, tissue culture, restoration, flavonoid, saponin
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